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Fertilization of the sea urchin egg is accompanied by the assembly of an extracellular glycoprotein coat, the fertilization membrane. Assembly of the fertilization membrane involves exocytosis of egg cortical granules, divalent cation-mediated association of exudate proteins with the egg glycocalyx (the vitelline layer), and cross-linking of the assembled structure by ovoperoxidase, a fertilization membrane component derived from the cortical granules. We have identified and isolated a new protein, which we call proteoliaisin, that appears to be responsible for inserting ovoperoxidase into the fertilization membrane. Proteoliaisin is a 250,000-Mr protein that binds ovoperoxidase in a Ca2+-dependent manner, with half-maximal binding at 50 microM Ca2+. Other divalent cations are less effective (Ba2+, Mn2+, and Sr2+) or ineffective (Mg2+ and Cd2+) in mediating the binding interaction. Binding is optimal over the physiological pH range of fertilization membrane assembly (pH 5.5-7.5). Both proteoliaisin and ovoperoxidase are found in isolated, uncross-linked fertilization membranes. We have identified several macromolecular aggregates that are released from uncross-linked fertilization membranes after dilution into divalent cation-free buffer. One of these is an ovoperoxidase-proteoliaisin complex that is further disrupted only upon the addition of EGTA. These results suggest that a Ca2+-stabilized complex of ovoperoxidase and proteoliaisin forms one structural subunit of the fertilization membrane.
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976, Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Bryan,
The isolation of a major structural element of the sea urchin fertilization membrane.
1970,
Pubmed
,
Echinobase
Bryan,
On the reconstitution of the crystalline components of the sea urchin fertilization membrane.
1970,
Pubmed
,
Echinobase
Carroll,
Isolation and biological activity of the proteases released by sea urchin eggs following fertilization.
1975,
Pubmed
,
Echinobase
Chandler,
The vitelline layer of the sea urchin egg and its modification during fertilization. A freeze-fracture study using quick-freezing and deep-etching.
1980,
Pubmed
,
Echinobase
Deits,
Purification and properties of ovoperoxidase, the enzyme responsible for hardening the fertilization membrane of the sea urchin egg.
1984,
Pubmed
,
Echinobase
Fodor,
Isolation of a protease from sea urchin eggs before and after fertilization.
1975,
Pubmed
,
Echinobase
Foerder,
Release of ovoperoxidase from sea urchin eggs hardens the fertilization membrane with tyrosine crosslinks.
1977,
Pubmed
,
Echinobase
Gundersen,
Hapten-mediated immunopurification of membrane proteins labeled with fluorescein derivatives.
1984,
Pubmed
,
Echinobase
Hall,
Hardening of the sea urchin fertilization envelope by peroxidase-catalyzed phenolic coupling of tyrosines.
1978,
Pubmed
,
Echinobase
Inoue,
Fine structure of the fertilization membranes of sea urchin embryos.
1971,
Pubmed
,
Echinobase
Johnson,
Intracellular pH and activation of sea urchin eggs after fertilisation.
1976,
Pubmed
,
Echinobase
Kane,
Hyalin release during normal sea urchin development and its replacement after removal at fertilization.
1973,
Pubmed
,
Echinobase
Kay,
Assembly of the fertilization membrane of the sea urchin: isolation of a divalent cation-dependent intermediate and its crosslinking in vitro.
1982,
Pubmed
,
Echinobase
Klebanoff,
Metabolic similarities between fertilization and phagocytosis. Conservation of a peroxidatic mechanism.
1979,
Pubmed
,
Echinobase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
MEHL,
Acid and base production at fertilization in the sea urchin.
1961,
Pubmed
,
Echinobase
Morrissey,
Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.
1981,
Pubmed
Shapiro,
Molecular approaches to the study of fertilization.
1981,
Pubmed
Talbot,
The purification and characterization of an exo-beta (1 going to 3)-glucanohydrolase from sea urchin eggs.
1982,
Pubmed
,
Echinobase
Veron,
Sequential biochemical and morphological events during assembly of the fertilization membrane of the sea urchin.
1977,
Pubmed
,
Echinobase
