ECB-ART-36150
FEBS Lett
1993 Aug 23;3291-2:210-4. doi: 10.1016/0014-5793(93)80223-h.
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Ionic bases of the membrane potential and intracellular pH changes induced by speract in swollen sea urchin sperm.
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Signal transduction initiated by the egg peptide, speract, in sea urchin sperm is not fully understood. Hypotonically swollen sperm are a suitable model to study peptide signal transduction. Ion substitution experiments now indicate (i) that the permeability to Na+, Ca2+, and Mg2+ contributes to the sperm resting membrane potential; (ii) the repolarization induced by nM concentrations of speract is Na+ dependent and mediated by an as yet unidentified channel; (iii) the depolarization triggered by nM concentrations of speract involves Ca2+ channels since it is Ca(2+)-dependent and blocked by Co2+ and Ni2+, two Ca2+ channel blockers; (iv) hyperpolarizing swollen sperm with valinomycin increases intracellular pH (pHi) in the same way as speract, thus the speract-induced hyperpolarization may be responsible for the pHi increase.
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Genes referenced: LOC100887844 LOC576642