Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Cell Tissue Res
1983 Jan 01;2342:309-18. doi: 10.1007/bf00213770.
Show Gene links
Show Anatomy links
Microinjection of Lucifer yellow CH into sea urchin eggs and embryos.
Pochapin MB
,
Sanger JM
,
Sanger JW
.
???displayArticle.abstract???
Eggs and embryos of Arbacia punctulata were microinjected with the fluorescent dye, Lucifer yellow CH, using a simple pressure injection system. When injected into eggs that were subsequently fertilized, the dye was distributed throughout all cells of the developing embryo. If one cell of a two-cell embryo was injected, dye did not diffuse into the uninjected blastomere. During subsequent development, all progeny of the injected cell contained dye resulting in an embryo that was half-fluorescent. Blue light irradiation of a two-cell embryo, one cell of which had been injected with Lucifer yellow, caused the injected blastomere to stop further divisions while the uninjected blastomere developed normally and was free of dye. These results indicate that the first two blastomeres of Arbacia embryos are not electrically coupled, nor up to the time of hatching, is there any coupling between cells in one half of the first cleavage plane and cells in the other half.
???displayArticle.pubmedLink???
6640624
???displayArticle.link???Cell Tissue Res ???displayArticle.grants???[+]
