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ECB-ART-42392
Dev Dyn 2012 Jun 01;2416:1111-24. doi: 10.1002/dvdy.23786.
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ATP-binding cassette (ABC) transporter expression and localization in sea urchin development.

Shipp LE , Hamdoun A .


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BACKGROUND: ATP-binding cassette (ABC) transporters are membrane proteins that regulate intracellular concentrations of myriad compounds and ions. There are >100 ABC transporter predictions in the Strongylocentrotus purpuratus genome, including 40 annotated ABCB, ABCC, and ABCG "multidrug efflux" transporters. Despite the importance of multidrug transporters for protection and signaling, their expression patterns have not been characterized in deuterostome embryos. RESULTS: Sea urchin embryos expressed 20 ABCB, ABCC, and ABCG transporter genes in the first 58 hr of development, from unfertilized egg to early prism. We quantified transcripts of ABCB1a, ABCB4a, ABCC1, ABCC5a, ABCC9a, and ABCG2b, and found that ABCB1a mRNA was 10-100 times more abundant than other transporter mRNAs. In situ hybridization showed ABCB1a was expressed ubiquitously in embryos, while ABCC5a was restricted to secondary mesenchyme cells and their precursors. Fluorescent protein fusions showed localization of ABCB1a on apical cell surfaces, and ABCC5a on basolateral surfaces. CONCLUSIONS: Embryos use many ABC transporters with predicted functions in cell signaling, lysosomal and mitochondrial homeostasis, potassium channel regulation, pigmentation, and xenobiotic efflux. Detailed characterization of ABCB1a and ABCC5a revealed that they have different temporal and spatial gene expression profiles and protein localization patterns that correlate to their predicted functions in protection and development, respectively.

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Genes referenced: abcb1 abcb4 abcb6 abcc2 abcc6 ABCC9a LOC100887844 LOC105438508 LOC115919910 LOC115924212 LOC115925116 LOC575116 LOC578413 LOC582918

References [+] :
Aller, Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding. 2009, Pubmed