ECB-ART-35153
Proc Natl Acad Sci U S A
1981 Jan 01;781:377-81. doi: 10.1073/pnas.78.1.377.
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Cooperation of kinetochores and pole in the establishment of monopolar mitotic apparatus.
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Monopolar mitotic apparatus can be produced in sea urchin eggs by a manoeuvre that distributes the four poles of the second mitosis into four separate blastomeres. The pole of the monopolar mitotic apparatus generates a half-spindle that is similar in structural details to the half-spindle of a normal bipolar mitotic apparatus, although the chromosomes are not as well aligned as in a normal metaphase plate. The chromosomes are oriented; one kinetochore faces the pole while its sister kinetochore faces away from the pole. The poleward kinetochore is connected to the pole by bundles of microtubules. No microtubules are seen on the sister kinetochore that faces away from the pole. Therefore, a single pole can direct most of the events in the establishment of a mitotic apparatus. Our interpretation examines the cooperation of kinetochores and poles in the formation of microtubules between them, stressing the half-spindle as the medium of cooperation and leaving open the question whether the kinetochores are origins or terminations of microtubules.
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Genes referenced: LOC100887844 pole
References [+] :
Andersen,
Specific visualization of the distribution of the calcium dependent regulatory protein of cyclic nucleotide phosphodiesterase (modulator protein) in tissue culture cells by immunofluorescence microscopy: mitosis and intercellular bridge.
1978, Pubmed