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Molecules
2020 Jun 08;2511:. doi: 10.3390/molecules25112663.
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Response Factorial Design Analysis on Papain-Generated Hydrolysates from Actinopyga lecanora for Determination of Antioxidant and Antityrosinase Activities.
Bahari AN
,
Saari N
,
Salim N
,
Ashari SE
.
Abstract
Actinopyga lecanora (A. lecanora) is classified among the edible species of sea cucumber, known to be rich in protein. Its hydrolysates were reported to contain relatively high antioxidant activity. Antioxidants are one of the essential properties in cosmeceutical products especially to alleviate skin aging. In the present study, pH, reaction temperature, reaction time and enzyme/substrate ratio (E/S) have been identified as the parameters in the papain enzymatic hydrolysis of A. lecanora. The degree of hydrolysis (DH) with antioxidant activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric-reducing antioxidant power (FRAP) assays were used as the responses in the optimization. Analysis of variance (ANOVA), normal plot of residuals and 3D contour plots were evaluated to study the effects and interactions between parameters. The best conditions selected from the optimization were at pH 5.00, 70 °C of reaction temperature, 9 h of hydrolysis time and 1.00% enzyme/substrate (E/S) ratio, with the hydrolysates having 51.90% of DH, 42.70% of DPPH activity and 109.90 Fe2+μg/mL of FRAP activity. The A. lecanora hydrolysates (ALH) showed a high amount of hydrophobic amino acids (286.40 mg/g sample) that might be responsible for antioxidant and antityrosinase activities. Scanning electron microscopy (SEM) image of ALH shows smooth structures with pores. Antityrosinase activity of ALH exhibited inhibition of 31.50% for L-tyrosine substrate and 25.40% for L-DOPA substrate. This condition suggests that the optimized ALH acquired has the potential to be used as a bioactive ingredient for cosmeceutical applications.
Figure 1. Normal plot of residual for (a) degree of hydrolysis (DH), (b) 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and (c) ferric-reducing antioxidant power (FRAP) activity.
Figure 2. Three-dimensional (3D) contour plots showing the influence of variable parameters on the degree of hydrolysis (DH) response. (a) pH vs. reaction temperature, (b) reaction time vs. E/S ratio and (c) pH vs. reaction time.
Figure 3. Cube plots showing the interaction of three significant effects on (a) the degree of hydrolysis (DH) response, (b) 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging response and (c) ferric-reducing antioxidant power (FRAP) response.
Figure 4. 3D contour plots showing the influence of variable parameters on the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging response. (a) pH vs. reaction time, (b) pH vs. reaction temperature and (c) reaction temperature vs. E/S ratio.
Figure 5. 3D contour plots showing the influence of variable parameters on the ferric-reducing antioxidant power (FRAP) response. (a) pH vs. E/S ratio, (b) reaction temperature vs. E/S ratio and (c) reaction time vs. E/S ratio.
Figure 6. Scanning electron microscopy (SEM) image of papain-generated hydrolysates from Actinopyga lecanora.
Figure 7. Tyrosinase inhibition activity for an optimized A. lecanora hydrolysates sample.
Auwal,
Response Surface Optimisation for the Production of Antioxidant Hydrolysates from Stone Fish Protein Using Bromelain.
2017, Pubmed
Auwal,
Response Surface Optimisation for the Production of Antioxidant Hydrolysates from Stone Fish Protein Using Bromelain.
2017,
Pubmed
Bordbar,
The improvement of the endogenous antioxidant property of stone fish (Actinopyga lecanora) tissue using enzymatic proteolysis.
2013,
Pubmed
,
Echinobase
Chen,
Discovery of highly potent tyrosinase inhibitor, T1, with significant anti-melanogenesis ability by zebrafish in vivo assay and computational molecular modeling.
2015,
Pubmed
Che Sulaiman,
Effects of temperature, time, and solvent ratio on the extraction of phenolic compounds and the anti-radical activity of Clinacanthus nutans Lindau leaves by response surface methodology.
2017,
Pubmed
Chi,
Antioxidant and functional properties of collagen hydrolysates from Spanish mackerel skin as influenced by average molecular weight.
2014,
Pubmed
Daliri,
Bioactive Peptides.
2017,
Pubmed
Ghanbari,
Actinopyga lecanora hydrolysates as natural antibacterial agents.
2012,
Pubmed
,
Echinobase
Ghanbari,
Angiotensin-I Converting Enzyme (ACE) Inhibitory and Anti-Oxidant Activities of Sea Cucumber (Actinopyga lecanora) Hydrolysates.
2015,
Pubmed
,
Echinobase
Guo,
Protein Hydrolysate from Pterygoplichthys disjunctivus, Armoured Catfish, with High Antioxidant Activity.
2019,
Pubmed
Kala,
Development and optimization of psychological stress model in mice using 2 level full factorial design.
2016,
Pubmed
León-López,
Hydrolysed Collagen from Sheepskins as a Source of Functional Peptides with Antioxidant Activity.
2019,
Pubmed
Muhammad Auwal,
Optimization of Bromelain-Aided Production of Angiotensin I-Converting Enzyme Inhibitory Hydrolysates from Stone Fish Using Response Surface Methodology.
2017,
Pubmed
,
Echinobase
Nakchum,
Preparation of squid skin collagen hydrolysate as an antihyaluronidase, antityrosinase, and antioxidant agent.
2016,
Pubmed
Nasri,
Protein Hydrolysates and Biopeptides: Production, Biological Activities, and Applications in Foods and Health Benefits. A Review.
2017,
Pubmed
Pongkai,
Effects of protein hydrolysate from chicken feather meal on tyrosinase activity and melanin formation in B16F10 murine melanoma cells.
2017,
Pubmed
Rajurkar,
Estimation of phytochemical content and antioxidant activity of some selected traditional Indian medicinal plants.
2011,
Pubmed
Sonklin,
Assessment of antioxidant properties of membrane ultrafiltration peptides from mungbean meal protein hydrolysates.
2018,
Pubmed
Wan Mohtar,
Preparation of bioactive peptides with high angiotensin converting enzyme inhibitory activity from winged bean [Psophocarpus tetragonolobus (L.) DC.] seed.
2014,
Pubmed
Zhang,
Cosmeceuticals and peptides.
2009,
Pubmed
Zhuang,
Optimization of antioxidant activity by response surface methodology in hydrolysates of jellyfish (Rhopilema esculentum) umbrella collagen.
2009,
Pubmed
Zolghadri,
A comprehensive review on tyrosinase inhibitors.
2019,
Pubmed