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???displayArticle.abstract??? Dysferlin is a calcium-binding transmembrane protein involved in membrane fusion and membrane repair. In humans, mutations in the dysferlin gene are associated with muscular dystrophy. In this study, we isolated plasma membrane-enriched fractions from full-grown immature oocytes of the sea star, and identified dysferlin by mass spectrometry analysis. The full-length dysferlin sequence is highly conserved between human and the sea star. We learned that in the sea star Patiria miniata, dysferlin RNA and protein are expressed from oogenesis to gastrulation. Interestingly, the protein is highly enriched in the plasma membrane of oocytes. Injection of a morpholino against dysferlin leads to a decrease of endocytosis in oocytes, and to a developmental arrest during gastrulation. These results suggest that dysferlin is critical for normal endocytosis during oogenesis and for embryogenesis in the sea star and that this animal may be a useful model for studying the relationship of dysferlin structure as it relates to its function.
Achanzar,
A nematode gene required for sperm vesicle fusion.
1997, Pubmed
Achanzar,
A nematode gene required for sperm vesicle fusion.
1997,
Pubmed
Audas,
Immobilization of proteins in the nucleolus by ribosomal intergenic spacer noncoding RNA.
2012,
Pubmed
Azakir,
Dysferlin interacts with tubulin and microtubules in mouse skeletal muscle.
2010,
Pubmed
Bansal,
Dysferlin and the plasma membrane repair in muscular dystrophy.
2004,
Pubmed
Bashir,
A gene related to Caenorhabditis elegans spermatogenesis factor fer-1 is mutated in limb-girdle muscular dystrophy type 2B.
1998,
Pubmed
Bernatchez,
Myoferlin is critical for endocytosis in endothelial cells.
2009,
Pubmed
Blom,
Sequence and structure-based prediction of eukaryotic protein phosphorylation sites.
1999,
Pubmed
Brooks,
The major yolk protein of sea urchins is endocytosed by a dynamin-dependent mechanism.
2004,
Pubmed
,
Echinobase
Cheng,
Regulated trafficking of the MSP/Eph receptor during oocyte meiotic maturation in C. elegans.
2008,
Pubmed
Cho,
Membrane binding and subcellular targeting of C2 domains.
2006,
Pubmed
Covian-Nares,
Membrane wounding triggers ATP release and dysferlin-mediated intercellular calcium signaling.
2010,
Pubmed
,
Echinobase
de Morrée,
Dysferlin regulates cell adhesion in human monocytes.
2013,
Pubmed
Di Fulvio,
Dysferlin interacts with histone deacetylase 6 and increases alpha-tubulin acetylation.
2011,
Pubmed
Doherty,
Normal myoblast fusion requires myoferlin.
2005,
Pubmed
Duckert,
Prediction of proprotein convertase cleavage sites.
2004,
Pubmed
El-Jouni,
Vesicular traffic at the cell membrane regulates oocyte meiotic arrest.
2007,
Pubmed
Eng,
An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database.
1994,
Pubmed
Foltz,
Echinoderm eggs and embryos: procurement and culture.
2004,
Pubmed
,
Echinobase
Fresques,
Selective accumulation of germ-line associated gene products in early development of the sea star and distinct differences from germ-line development in the sea urchin.
2014,
Pubmed
,
Echinobase
Gupta,
Prediction of glycosylation across the human proteome and the correlation to protein function.
2002,
Pubmed
Hinman,
Developmental gene regulatory network architecture across 500 million years of echinoderm evolution.
2003,
Pubmed
,
Echinobase
Hinman,
Expression of AmKrox, a starfish ortholog of a sea urchin transcription factor essential for endomesodermal specification.
2003,
Pubmed
,
Echinobase
Hinman,
Expression and function of a starfish Otx ortholog, AmOtx: a conserved role for Otx proteins in endoderm development that predates divergence of the eleutherozoa.
2003,
Pubmed
,
Echinobase
Illa,
Distal anterior compartment myopathy: a dysferlin mutation causing a new muscular dystrophy phenotype.
2001,
Pubmed
Kanatani,
Isolation and indentification on meiosis inducing substance in starfish Asterias amurensis.
1969,
Pubmed
,
Echinobase
Kawahara,
Characterization of zebrafish dysferlin by morpholino knockdown.
2011,
Pubmed
Kobayashi,
Dysferlin and animal models for dysferlinopathy.
2012,
Pubmed
Lek,
Ferlins: regulators of vesicle fusion for auditory neurotransmission, receptor trafficking and membrane repair.
2012,
Pubmed
Lek,
Phylogenetic analysis of ferlin genes reveals ancient eukaryotic origins.
2010,
Pubmed
Leung,
Proteomic identification of dysferlin-interacting protein complexes in human vascular endothelium.
2011,
Pubmed
Li,
Myoferlin depletion in breast cancer cells promotes mesenchymal to epithelial shape change and stalls invasion.
2012,
Pubmed
Liu,
Dysferlin, a novel skeletal muscle gene, is mutated in Miyoshi myopathy and limb girdle muscular dystrophy.
1998,
Pubmed
Lowther,
Endocytosis in the mouse oocyte and its contribution to cAMP signaling during meiotic arrest.
2011,
Pubmed
Marchler-Bauer,
CDD: a Conserved Domain Database for the functional annotation of proteins.
2011,
Pubmed
Minokawa,
Expression patterns of four different regulatory genes that function during sea urchin development.
2004,
Pubmed
,
Echinobase
Mitaku,
Amphiphilicity index of polar amino acids as an aid in the characterization of amino acid preference at membrane-water interfaces.
2002,
Pubmed
Monigatti,
The Sulfinator: predicting tyrosine sulfation sites in protein sequences.
2002,
Pubmed
Ohsako,
The Drosophila misfire gene has an essential role in sperm activation during fertilization.
2003,
Pubmed
Opresko,
Receptor-mediated endocytosis in Xenopus oocytes. I. Characterization of the vitellogenin receptor system.
1987,
Pubmed
Pangršič,
Otoferlin: a multi-C2 domain protein essential for hearing.
2012,
Pubmed
Patel,
Solution structure of the inner DysF domain of myoferlin and implications for limb girdle muscular dystrophy type 2b.
2008,
Pubmed
Robinson,
While dysferlin and myoferlin are coexpressed in the human placenta, only dysferlin expression is responsive to trophoblast fusion in model systems.
2009,
Pubmed
Sharma,
A new role for the muscle repair protein dysferlin in endothelial cell adhesion and angiogenesis.
2010,
Pubmed
Smith,
Complex regulation and multiple developmental functions of misfire, the Drosophila melanogaster ferlin gene.
2007,
Pubmed
Sodergren,
The genome of the sea urchin Strongylocentrotus purpuratus.
2006,
Pubmed
,
Echinobase
Staub,
Insights into the evolution of the nucleolus by an analysis of its protein domain repertoire.
2004,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Tschochner,
Pre-ribosomes on the road from the nucleolus to the cytoplasm.
2003,
Pubmed
Washington,
FER-1 regulates Ca2+ -mediated membrane fusion during C. elegans spermatogenesis.
2006,
Pubmed
Wessel,
Use of sea stars to study basic reproductive processes.
2010,
Pubmed
,
Echinobase
Xu,
Dysferlin forms a dimer mediated by the C2 domains and the transmembrane domain in vitro and in living cells.
2011,
Pubmed
Yasunaga,
A mutation in OTOF, encoding otoferlin, a FER-1-like protein, causes DFNB9, a nonsyndromic form of deafness.
1999,
Pubmed
