Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Dev Dyn
2012 Jun 01;2416:1111-24. doi: 10.1002/dvdy.23786.
Show Gene links
Show Anatomy links
ATP-binding cassette (ABC) transporter expression and localization in sea urchin development.
Shipp LE
,
Hamdoun A
.
???displayArticle.abstract???
BACKGROUND: ATP-binding cassette (ABC) transporters are membrane proteins that regulate intracellular concentrations of myriad compounds and ions. There are >100 ABC transporter predictions in the Strongylocentrotus purpuratus genome, including 40 annotated ABCB, ABCC, and ABCG "multidrug efflux" transporters. Despite the importance of multidrug transporters for protection and signaling, their expression patterns have not been characterized in deuterostome embryos.
RESULTS: Sea urchin embryos expressed 20 ABCB, ABCC, and ABCG transporter genes in the first 58 hr of development, from unfertilized egg to early prism. We quantified transcripts of ABCB1a, ABCB4a, ABCC1, ABCC5a, ABCC9a, and ABCG2b, and found that ABCB1a mRNA was 10-100 times more abundant than other transporter mRNAs. In situ hybridization showed ABCB1a was expressed ubiquitously in embryos, while ABCC5a was restricted to secondary mesenchyme cells and their precursors. Fluorescent protein fusions showed localization of ABCB1a on apical cell surfaces, and ABCC5a on basolateral surfaces.
CONCLUSIONS: Embryos use many ABC transporters with predicted functions in cell signaling, lysosomal and mitochondrial homeostasis, potassium channel regulation, pigmentation, and xenobiotic efflux. Detailed characterization of ABCB1a and ABCC5a revealed that they have different temporal and spatial gene expression profiles and protein localization patterns that correlate to their predicted functions in protection and development, respectively.
Aller,
Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding.
2009, Pubmed
Aller,
Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding.
2009,
Pubmed
Bangert,
The lysosomal polypeptide transporter TAPL: more than a housekeeping factor?
2011,
Pubmed
Borst,
Mammalian ABC transporters in health and disease.
2002,
Pubmed
Bosnjak,
Multidrug efflux transporters limit accumulation of inorganic, but not organic, mercury in sea urchin embryos.
2009,
Pubmed
,
Echinobase
Brandhorst,
Isolating DNA, RNA, polysomes, and protein.
2004,
Pubmed
,
Echinobase
Bryan,
ABCC8 and ABCC9: ABC transporters that regulate K+ channels.
2007,
Pubmed
Bunting,
ABC transporters as phenotypic markers and functional regulators of stem cells.
2002,
Pubmed
Cameron,
SpBase: the sea urchin genome database and web site.
2009,
Pubmed
,
Echinobase
Chen,
Abcb10 physically interacts with mitoferrin-1 (Slc25a37) to enhance its stability and function in the erythroid mitochondria.
2009,
Pubmed
Chen,
Multidrug resistance proteins (MRPs/ABCCs) in cancer chemotherapy and genetic diseases.
2011,
Pubmed
Coffman,
Oral-aboral axis specification in the sea urchin embryo III. Role of mitochondrial redox signaling via H2O2.
2009,
Pubmed
,
Echinobase
Dean,
The human ATP-binding cassette (ABC) transporter superfamily.
2001,
Pubmed
Elbling,
P-glycoprotein regulates chemosensitivity in early developmental stages of the mouse.
1993,
Pubmed
Evans,
A new role for a classical gene: white transports cyclic GMP.
2008,
Pubmed
Ewart,
Mutational analysis of the traffic ATPase (ABC) transporters involved in uptake of eye pigment precursors in Drosophila melanogaster. Implications for structure-function relationships.
1994,
Pubmed
Fjose,
Developmental expression of the white locus of Drosophila melanogaster.
1984,
Pubmed
Fletcher,
ABC transporters in cancer: more than just drug efflux pumps.
2010,
Pubmed
Foltz,
Echinoderm eggs and embryos: procurement and culture.
2004,
Pubmed
,
Echinobase
Goldstone,
The chemical defensome: environmental sensing and response genes in the Strongylocentrotus purpuratus genome.
2006,
Pubmed
,
Echinobase
Hamdoun,
Activation of multidrug efflux transporter activity at fertilization in sea urchin embryos (Strongylocentrotus purpuratus).
2004,
Pubmed
,
Echinobase
He,
Structural and functional properties of human multidrug resistance protein 1 (MRP1/ABCC1).
2011,
Pubmed
Herget,
Intracellular peptide transporters in human--compartmentalization of the "peptidome".
2007,
Pubmed
Higgins,
ABC transporters: from microorganisms to man.
1992,
Pubmed
Itoh,
Membrane-bound transporter controls the circadian transcription of clock genes in Drosophila.
2011,
Pubmed
Jedlitschky,
The multidrug resistance protein 5 functions as an ATP-dependent export pump for cyclic nucleotides.
2000,
Pubmed
Juliano,
Germ line determinants are not localized early in sea urchin development, but do accumulate in the small micromere lineage.
2006,
Pubmed
,
Echinobase
Krishnamurthy,
Role of ABCG2/BCRP in biology and medicine.
2006,
Pubmed
Lepage,
Expression of exogenous mRNAs to study gene function in the sea urchin embryo.
2004,
Pubmed
,
Echinobase
Leslie,
Toxicological relevance of the multidrug resistance protein 1, MRP1 (ABCC1) and related transporters.
2001,
Pubmed
Leslie,
Multidrug resistance proteins: role of P-glycoprotein, MRP1, MRP2, and BCRP (ABCG2) in tissue defense.
2005,
Pubmed
Mackenzie,
Sub-cellular localisation of the white/scarlet ABC transporter to pigment granule membranes within the compound eye of Drosophila melanogaster.
2000,
Pubmed
Nam,
Cis-regulatory control of the nodal gene, initiator of the sea urchin oral ectoderm gene network.
2007,
Pubmed
,
Echinobase
Nasonkin,
A novel sulfonylurea receptor family member expressed in the embryonic Drosophila dorsal vessel and tracheal system.
1999,
Pubmed
Nemer,
Developmental regulation, induction, and embryonic tissue specificity of sea urchin metallothionein gene expression.
1984,
Pubmed
,
Echinobase
Nemer,
Polyubiquitin RNA characteristics and conditional induction in sea urchin embryos.
1991,
Pubmed
,
Echinobase
Peter,
Genomic control of patterning.
2009,
Pubmed
,
Echinobase
Peter,
The endoderm gene regulatory network in sea urchin embryos up to mid-blastula stage.
2010,
Pubmed
,
Echinobase
Petrini,
Expression of multidrug resistance (mdr) gene(s) in primary lymphoid organs of chicken immune system during embryonic development.
1995,
Pubmed
Ransick,
New early zygotic regulators expressed in endomesoderm of sea urchin embryos discovered by differential array hybridization.
2002,
Pubmed
,
Echinobase
Ransick,
Detection of mRNA by in situ hybridization and RT-PCR.
2004,
Pubmed
,
Echinobase
Revilla-i-Domingo,
A missing link in the sea urchin embryo gene regulatory network: hesC and the double-negative specification of micromeres.
2007,
Pubmed
,
Echinobase
Ricardo,
An ABC transporter controls export of a Drosophila germ cell attractant.
2009,
Pubmed
Samanta,
The transcriptome of the sea urchin embryo.
2006,
Pubmed
,
Echinobase
Saxena,
Transcription factors that mediate epithelial-mesenchymal transition lead to multidrug resistance by upregulating ABC transporters.
2011,
Pubmed
Schmittgen,
Analyzing real-time PCR data by the comparative C(T) method.
2008,
Pubmed
Smeets,
A low but functionally significant MDR1 expression protects primitive haemopoietic progenitor cells from anthracycline toxicity.
1997,
Pubmed
Stieger,
The bile salt export pump.
2007,
Pubmed
Susanto,
Porphyrin homeostasis maintained by ABCG2 regulates self-renewal of embryonic stem cells.
2008,
Pubmed
Toomey,
Multixenobiotic Resistance in Urechis caupo Embryos: Protection From Environmental Toxins.
1993,
Pubmed
,
Echinobase
Wu,
Ingression of primary mesenchyme cells of the sea urchin embryo: a precisely timed epithelial mesenchymal transition.
2007,
Pubmed
,
Echinobase
Wu,
Isolation and characterization of Drosophila multidrug resistance gene homologs.
1991,
Pubmed
Yabe,
Multidrug resistance-associated protein MRP-1 regulates dauer diapause by its export activity in Caenorhabditis elegans.
2005,
Pubmed
Zhao,
TAP and TAP-like--brothers in arms?
2006,
Pubmed
Zhou,
The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype.
2001,
Pubmed
Zutz,
Mitochondrial ABC proteins in health and disease.
2009,
Pubmed
de Wolf,
cGMP transport by vesicles from human and mouse erythrocytes.
2007,
Pubmed
