Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Lipids Health Dis
2010 Jul 08;9:68. doi: 10.1186/1476-511X-9-68.
Show Gene links
Show Anatomy links
Soybean and fish oil mixture increases IL-10, protects against DNA damage and decreases colonic inflammation in rats with dextran sulfate sodium (DSS) colitis.
Barros KV
,
Xavier RA
,
Abreu GG
,
Martinez CA
,
Ribeiro ML
,
Gambero A
,
Carvalho PO
,
Nascimento CM
,
Silveira VL
.
Abstract
It was investigated whether dietary polyunsaturated fatty acids (PUFA) could influence colonic injury, tissue DNA damage, cytokines and myeloperoxidase activity (MPO) and plasma corticosterone in DSS-induced colitis rats. Male weaning Wistar rats were fed for 47 days with an AIN-93 diet with control (C), fish (F) or a mixture of fish and soybean oil (SF). The colitis was induced from day 36 until day 42 by 3% DSS in drinking water. On day 48, blood samples were collected for corticosterone determination. The distal colon was excised for histological analysis and to quantify the cytokine (IL-4, IL-10 and INF-gamma), MPO and DNA damage. The disease activity index (DAI) was recorded daily during colitis induction. The DAI, MPO, histological analyses showed decreases only in the SF group compared with the C group. IL-10 was increased and DNA damage was reduced in the groups F and SF, and an inverse correlation between these variables was found. There were no differences in corticosterone, IFN-gamma and IL-4 levels. Soybean and fish oil mixture may be effective in improving colonic injury and DNA damage, and it could be an important complementary therapy in UC to reduce the use of anti-inflammatory drugs and prevent colorectal cancer.
Figure 2. Histology (hematoxylin-eosin, magnification, × 200) of colonic samples taken from Wistar rats receiving 3% DSS for 7 days and water for 5 days. (A) Control group, fed control diet, showed ulceration of epithelial superficies (black arrow), intense inflammatory cellular infiltration (white arrow) and destruction of colonic architecture; (B) Fish group, fed fish diet, showed basal lamina edema and moderate cellular infiltrate (arrow); (C) Soybean/Fish group, fed soybean/fish diet showed light cellular infiltrate (arrow). The soybean/fish diet was more efficient than the fish diet in attenuating morphologic damage and preserving colonic architecture.
Figure 3. Correlation between DNA damage levels (tail moment/100 cells isolated from colon) and tissue IL-10 concentration (ρg/mL) in all DSS-induced colitis rats.
Bancroft,
Dietary fish oil reduces oxidative DNA damage in rat colonocytes.
2003, Pubmed
Bancroft,
Dietary fish oil reduces oxidative DNA damage in rat colonocytes.
2003,
Pubmed
Belluzzi,
Polyunsaturated fatty acids and inflammatory bowel disease.
2000,
Pubmed
Belluzzi,
N-3 fatty acids for the treatment of inflammatory bowel diseases.
2002,
Pubmed
Calder,
Immunomodulation by omega-3 fatty acids.
2007,
Pubmed
Calder,
Polyunsaturated fatty acids, inflammatory processes and inflammatory bowel diseases.
2008,
Pubmed
Camuesco,
Dietary olive oil supplemented with fish oil, rich in EPA and DHA (n-3) polyunsaturated fatty acids, attenuates colonic inflammation in rats with DSS-induced colitis.
2005,
Pubmed
Chapkin,
Immunomodulatory effects of (n-3) fatty acids: putative link to inflammation and colon cancer.
2007,
Pubmed
Cooper,
Clinicopathologic study of dextran sulfate sodium experimental murine colitis.
1993,
Pubmed
Courtney,
Eicosapentaenoic acid (EPA) reduces crypt cell proliferation and increases apoptosis in normal colonic mucosa in subjects with a history of colorectal adenomas.
2007,
Pubmed
Dieleman,
Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines.
1998,
Pubmed
FOLCH,
A simple method for the isolation and purification of total lipides from animal tissues.
1957,
Pubmed
Figler,
Association of n-3 and n-6 long-chain polyunsaturated fatty acids in plasma lipid classes with inflammatory bowel diseases.
2007,
Pubmed
GUILLEMIN,
Fluorometric measurement of rat plasma and adrenal corticosterone concentration; a note on technical details.
1959,
Pubmed
Gambero,
Mesenteric adipose tissue alterations resulting from experimental reactivated colitis.
2007,
Pubmed
Gil,
Polyunsaturated fatty acids and inflammatory diseases.
2002,
Pubmed
Groux,
A CD4+ T-cell subset inhibits antigen-specific T-cell responses and prevents colitis.
1997,
Pubmed
Hegazi,
Dietary fatty acids modulate chronic colitis, colitis-associated colon neoplasia and COX-2 expression in IL-10 knockout mice.
2006,
Pubmed
Hudert,
Transgenic mice rich in endogenous omega-3 fatty acids are protected from colitis.
2006,
Pubmed
Kuroki,
Serum n3 polyunsaturated fatty acids are depleted in Crohn's disease.
1997,
Pubmed
Lindsay,
IL-10 gene therapy is therapeutic for dextran sodium sulfate-induced murine colitis.
2004,
Pubmed
Ma,
High-fat diet modulates non-CD1d-restricted natural killer T cells and regulatory T cells in mouse colon and exacerbates experimental colitis.
2008,
Pubmed
McGuckin,
Intestinal barrier dysfunction in inflammatory bowel diseases.
2009,
Pubmed
Nieto,
Dietary monounsaturated n-3 and n-6 long-chain polyunsaturated fatty acids affect cellular antioxidant defense system in rats with experimental ulcerative colitis induced by trinitrobenzene sulfonic acid.
1998,
Pubmed
Razack,
Nutrition in inflammatory bowel disease.
2007,
Pubmed
Reed,
NF-kappaB activation precedes increases in mRNA encoding neurokinin-1 receptor, proinflammatory cytokines, and adhesion molecules in dextran sulfate sodium-induced colitis in rats.
2005,
Pubmed
Reeves,
Components of the AIN-93 diets as improvements in the AIN-76A diet.
1997,
Pubmed
Ribeiro,
Analysis of oxidative DNA damage in patients with colorectal cancer.
2008,
Pubmed
Roediger,
The starved colon--diminished mucosal nutrition, diminished absorption, and colitis.
1990,
Pubmed
Roessner,
Oxidative stress in ulcerative colitis-associated carcinogenesis.
2008,
Pubmed
Sanchez-Munoz,
Role of cytokines in inflammatory bowel disease.
2008,
Pubmed
Silveira,
Participation of the adrenal gland in the anti-inflammatory effect of polyunsaturated diets.
1995,
Pubmed
Simopoulos,
Omega-3 fatty acids in inflammation and autoimmune diseases.
2002,
Pubmed
Simopoulos,
Importance of the ratio of omega-6/omega-3 essential fatty acids: evolutionary aspects.
2003,
Pubmed
Singh,
A simple technique for quantitation of low levels of DNA damage in individual cells.
1988,
Pubmed
Tardieu,
Dextran sulfate enhances the level of an oxidative DNA damage biomarker, 8-oxo-7,8-dihydro-2'-deoxyguanosine, in rat colonic mucosa.
1998,
Pubmed
Trebble,
High-dose fish oil and antioxidants in Crohn's disease and the response of bone turnover: a randomised controlled trial.
2005,
Pubmed
Wohlers,
Effect of fish or soybean oil-rich diets on bradykinin, kallikrein, nitric oxide, leptin, corticosterone and macrophages in carrageenan stimulated rats.
2005,
Pubmed
