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Echinobase
ECB-ART-51843
Dev Growth Differ 1980 Jan 01;223:429-436. doi: 10.1111/j.1440-169X.1980.00429.x.
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INHIBITION OF RESPIRATION IN SEA URCHIN SPERMATOZOA FOLLOWING INTERACTION WITH FIXED UNFERTILIZED EGGS: II. CAPACITY OF THE GLUTARALDEHYDE FIXED UNFERTILIZED EGGS FOR THE INHIBITION OF THE SPERM RESPIRATION.

Yasumasu I , Fujiwara A , Hino A .


Abstract
Glutaradehyde-fixed unfertilized eggs of the sea urchin, Pseudocentrotus depressus, which bound spermatozoa, were capable of causing an inhibition of respiration in 2.3 ± 0.27 × 104 spermatozoa per egg. After stirring a suspension containing fixed eggs (2 × 103 -2 × 104 per ml) and a larger number of spematozoa than 2.5 × 104 per egg for 15 min at 20°c, the fixed eggs isolated from the suspension failed to cause any inhibition of respiration of fresh spermatozoa, and were incapable of binding fresh eggs. Fixed eggs, treated with a smaller number of spermatozoa than 2 × 104 per egg, had a capacity to bind spermatozoa and inhibited sperm respiration. Unless the fixed eggs lost the sperm-binding capacity, they exerted an inhibitory effect on the respiration of spermatozoa.

PubMed ID: 37281047
Article link: Dev Growth Differ