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ECB-ART-42788
Oecologia 1989 Mar 01;801:82-6. doi: 10.1007/BF00789935.
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Analysis of feeding preference experiments.

Peterson CH , Renaud PE .


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Published studies of consumer feeding preferences using foods that experience autogenic change in mass, numbers, area, etc., on the time scale of a feeding trial fail to employ appropriate statistical analyses to incorporate controls for those food changes occurring in the absence of the consumer. The studies that run controls typically use them to calculate a constant "correction factor", which is subtracted prior to formal data analysis. This procedure constitutes a non-rigorous suppression of variance that overstates the statistical significance of observed differences. The appropriate statistical analysis for preference tests with two foods is usually a simple t-test performed on the between-food differences in loss of mass (or numbers, area, etc.) comparing the results of experimentals with consumers to controls without consumers. Application of this recommended test procedure to an actual data set illustrates how low replication in controls, which is typical of most studies of feeding preference, inhibits detection of an apparently large influence of previous mechanical damage (simulated grazing) in reducing the attractiveness of a brown alga to a sea urchin.

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Genes referenced: LOC100887844 LOC115925415 LOC583082

References [+] :
Geiselman, Polyphenols in brown algaeFucus vesiculosus andAscophyllum nodosum: Chemical defenses against the marine herbivorous snail,Littorina littorea. 1981, Pubmed