ECB-ART-41429Development 2010 Jan 01;1371:83-91. doi: 10.1242/dev.044149.
Show Gene links Show Anatomy links
Dynamics of Delta/Notch signaling on endomesoderm segregation in the sea urchin embryo.
Endomesoderm is the common progenitor of endoderm and mesoderm early in the development of many animals. In the sea urchin embryo, the Delta/Notch pathway is necessary for the diversification of this tissue, as are two early transcription factors, Gcm and FoxA, which are expressed in mesoderm and endoderm, respectively. Here, we provide a detailed lineage analysis of the cleavages leading to endomesoderm segregation, and examine the expression patterns and the regulatory relationships of three known regulators of this cell fate dichotomy in the context of the lineages. We observed that endomesoderm segregation first occurs at hatched blastula stage. Prior to this stage, Gcm and FoxA are co-expressed in the same cells, whereas at hatching these genes are detected in two distinct cell populations. Gcm remains expressed in the most vegetal endomesoderm descendant cells, while FoxA is downregulated in those cells and activated in the above neighboring cells. Initially, Delta is expressed exclusively in the micromeres, where it is necessary for the most vegetal endomesoderm cell descendants to express Gcm and become mesoderm. Our experiments show a requirement for a continuous Delta input for more than two cleavages (or about 2.5 hours) before Gcm expression continues in those cells independently of further Delta input. Thus, this study provides new insights into the timing mechanisms and the molecular dynamics of endomesoderm segregation during sea urchin embryogenesis and into the mode of action of the Delta/Notch pathway in mediating mesoderm fate.
PubMed ID: 20023163
PMC ID: PMC2796929
Article link: Development
Genes referenced: foxa1 gcml LOC100887844 LOC115919910 LOC115921237
References [+] :
Bally-Cuif, Coregulation of anterior and posterior mesendodermal development by a hairy-related transcriptional repressor. 2000, Pubmed