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Dev Genes Evol
2006 May 01;2165:265-76. doi: 10.1007/s00427-005-0051-6.
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Ion flow regulates left-right asymmetry in sea urchin development.
Hibino T
,
Ishii Y
,
Levin M
,
Nishino A
.
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The degree of conservation among phyla of early mechanisms that pattern the left-right (LR) axis is poorly understood. Larvae of sea urchins exhibit consistently oriented LR asymmetry. The main part of the adult rudiment is formed from the left coelomic sac of larvae, the left hydrocoel. Although this left preference is conserved among all echinoderm larvae, its mechanism is largely not understood. Using two marker genes, HpNot and HpFoxFQ-like, which are asymmetrically expressed during larval development of the sea urchin Hemicentrotus pulcherrimus, we examined in this study the possibility that the recently discovered ion flux mechanism controls asymmetry in sea urchins as it does in several vertebrate species. Several ion-transporter inhibitors were screened for the ability to alter the expression of the asymmetric marker genes. Blockers of the H(+)/K(+)-ATPase (omeprazole, lansoprazole and SCH28080), as well as a calcium ionophore (A23187), significantly altered the normal sidedness of asymmetric gene expression. Exposure to omeprazole disrupted the consistent asymmetry of adult rudiment formation in larvae. Immuno-detection revealed that H(+)/K(+)-ATPase-like antigens in sea urchin embryos were present through blastula stage and exhibited a striking asymmetry being present in a single blastomere in 32-cell embryos. These results suggest that, as in vertebrates, endogenous spatially-regulated early transport of H(+) and/or K(+), and also of Ca(2+), functions in the establishment of LR asymmetry in sea urchin development.
Aihara,
Left-right positioning of the adult rudiment in sea urchin larvae is directed by the right side.
2001, Pubmed,
Echinobase
Aihara,
Left-right positioning of the adult rudiment in sea urchin larvae is directed by the right side.
2001,
Pubmed
,
Echinobase
Akasaka,
Oral-aboral ectoderm differentiation of sea urchin embryos is disrupted in response to calcium ionophore.
1997,
Pubmed
,
Echinobase
Arenas-Mena,
Spatial expression of Hox cluster genes in the ontogeny of a sea urchin.
2000,
Pubmed
,
Echinobase
Asano,
The cavity structure for docking the K(+)-competitive inhibitors in the gastric proton pump.
2004,
Pubmed
Boorman,
The evolution of left-right asymmetry in chordates.
2002,
Pubmed
Coffman,
Oral-aboral axis specification in the sea urchin embryo II. Mitochondrial distribution and redox state contribute to establishing polarity in Strongylocentrotus purpuratus.
2004,
Pubmed
,
Echinobase
Coffman,
Oral-aboral axis specification in the sea urchin embryo. I. Axis entrainment by respiratory asymmetry.
2001,
Pubmed
,
Echinobase
Cooke,
The evolutionary origins and significance of vertebrate left-right organisation.
2004,
Pubmed
Davidson,
Glycyrrhetinic acid derivatives: a novel class of inhibitors of gap-junctional intercellular communication. Structure-activity relationships.
1988,
Pubmed
DuVall,
Peroxynitrite inhibits amiloride-sensitive Na+ currents in Xenopus oocytes expressing alpha beta gamma-rENaC.
1998,
Pubmed
Duboc,
Nodal and BMP2/4 signaling organizes the oral-aboral axis of the sea urchin embryo.
2004,
Pubmed
,
Echinobase
Duboc,
Left-right asymmetry in the sea urchin embryo is regulated by nodal signaling on the right side.
2005,
Pubmed
,
Echinobase
Gambino,
Spatial distribution of collagen type I mRNA in Paracentrotus lividus eggs and embryos.
1997,
Pubmed
,
Echinobase
Hamada,
Establishment of vertebrate left-right asymmetry.
2002,
Pubmed
Hamada,
Signals from primary mesenchyme cells regulate endoderm differentiation in the sea urchin embryo.
2003,
Pubmed
,
Echinobase
Harris,
Amiloride and the Na(+)/H(+) exchanger protein: mechanism and significance of inhibition of the Na(+)/H(+) exchanger (review).
1999,
Pubmed
Jiang,
Antileishmanial activity of the antiulcer agent omeprazole.
2002,
Pubmed
Kramer-Zucker,
Cilia-driven fluid flow in the zebrafish pronephros, brain and Kupffer's vesicle is required for normal organogenesis.
2005,
Pubmed
Landry,
Epithelial chloride channel. Development of inhibitory ligands.
1987,
Pubmed
Levin,
Two molecular models of initial left-right asymmetry generation.
1997,
Pubmed
Levin,
Motor protein control of ion flux is an early step in embryonic left-right asymmetry.
2003,
Pubmed
Levin,
Asymmetries in H+/K+-ATPase and cell membrane potentials comprise a very early step in left-right patterning.
2002,
Pubmed
Levin,
The embryonic origins of left-right asymmetry.
2004,
Pubmed
Levin,
A novel immunohistochemical method for evaluation of antibody specificity and detection of labile targets in biological tissue.
2004,
Pubmed
Levin,
Left-right asymmetry in embryonic development: a comprehensive review.
2005,
Pubmed
Logan,
Nuclear beta-catenin is required to specify vegetal cell fates in the sea urchin embryo.
1999,
Pubmed
,
Echinobase
McGrath,
Two populations of node monocilia initiate left-right asymmetry in the mouse.
2003,
Pubmed
Mercola,
Left-right asymmetry: nodal points.
2003,
Pubmed
Mitsunaga,
Distributions of H+,K+-ATPase and Cl-,HCO3(-)-ATPase in micromere-derived cells of sea urchin embryos.
1987,
Pubmed
,
Echinobase
Mukherjee,
Leishmania plasma membrane Mg2+-ATPase is a H+/K+-antiporter involved in glucose symport. Studies with sealed ghosts and vesicles of opposite polarity.
2001,
Pubmed
Munson,
Effects of mutations in M4 of the gastric H+,K+-ATPase on inhibition kinetics of SCH28080.
2000,
Pubmed
Møller,
Structural organization, ion transport, and energy transduction of P-type ATPases.
1996,
Pubmed
Nathan,
Selective block of calcium current by lanthanum in single bullfrog atrial cells.
1988,
Pubmed
Nonaka,
Determination of left-right patterning of the mouse embryo by artificial nodal flow.
2002,
Pubmed
Nonaka,
Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein.
1998,
Pubmed
Okada,
Mechanism of nodal flow: a conserved symmetry breaking event in left-right axis determination.
2005,
Pubmed
Peterson,
Bilaterian origins: significance of new experimental observations.
2000,
Pubmed
,
Echinobase
Ransick,
New early zygotic regulators expressed in endomesoderm of sea urchin embryos discovered by differential array hybridization.
2002,
Pubmed
,
Echinobase
Raya,
Notch activity acts as a sensor for extracellular calcium during vertebrate left-right determination.
2004,
Pubmed
Ryan,
Pitx2 determines left-right asymmetry of internal organs in vertebrates.
1998,
Pubmed
Sachs,
The pharmacology of the gastric acid pump: the H+,K+ ATPase.
1995,
Pubmed
Sanders,
The effects of lansoprazole, a new H+,K(+)-ATPase inhibitor, on gastric pH and serum gastrin.
1992,
Pubmed
Showman,
Removal of the fertilization membrane of sea urchin embryos employing aminotriazole.
1979,
Pubmed
,
Echinobase
Tanaka,
FGF-induced vesicular release of Sonic hedgehog and retinoic acid in leftward nodal flow is critical for left-right determination.
2005,
Pubmed
Toyoizumi,
Adrenergic neurotransmitters and calcium ionophore-induced situs inversus viscerum in Xenopus laevis embryos.
1997,
Pubmed
Vakil,
Review article: esomeprazole, 40 mg once daily, compared with lansoprazole, 30 mg once daily, in healing and symptom resolution of erosive oesophagitis.
2003,
Pubmed
Vlahou,
Maternal mRNA encoding the orphan steroid receptor SpCOUP-TF is localized in sea urchin eggs.
1996,
Pubmed
,
Echinobase
Weitzel,
Differential stability of beta-catenin along the animal-vegetal axis of the sea urchin embryo mediated by dishevelled.
2004,
Pubmed
,
Echinobase