Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Proc Natl Acad Sci U S A
1984 Feb 01;814:1057-61. doi: 10.1073/pnas.81.4.1057.
Show Gene links
Show Anatomy links
3'' editing of mRNAs: sequence requirements and involvement of a 60-nucleotide RNA in maturation of histone mRNA precursors.
Birchmeier C
,
Schümperli D
,
Sconzo G
,
Birnstiel ML
.
???displayArticle.abstract???
In vitro-synthesized transcripts of the sea urchin histone H2A gene with 3'' extensions are efficiently and rapidly processed to H2A mRNA with faithful 3'' ends in Xenopus laevis oocyte nuclei. Processing requires the presence of a histone-specific dyad symmetry element and of H2A-proximal spacer sequences in the precursor RNA. In DNA injection experiments with a processing-deficient H2A mutant, the transcription products appear to terminate heterogeneously in the first 100-200 base pairs of the post-H2A spacer. Processing of synthetic H3 RNA precursors requires the prior injection of a 60-nucleotide RNA from sea urchin embryos that seems to be a component of a small nuclear ribonucleoprotein.
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977, Pubmed
Berk,
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
1977,
Pubmed
Birchmeier,
Generation of authentic 3' termini of an H2A mRNA in vivo is dependent on a short inverted DNA repeat and on spacer sequences.
1982,
Pubmed
,
Echinobase
Birchmeier,
The terminal RNA stem-loop structure and 80 bp of spacer DNA are required for the formation of 3' termini of sea urchin H2A mRNA.
1983,
Pubmed
,
Echinobase
Contreras,
Simple, efficient in vitro synthesis of capped RNA useful for direct expression of cloned eukaryotic genes.
1982,
Pubmed
Darnell,
Variety in the level of gene control in eukaryotic cells.
1982,
Pubmed
Fitzgerald,
The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
1981,
Pubmed
Galli,
Biochemical complementation with RNA in the Xenopus oocyte: a small RNA is required for the generation of 3' histone mRNA termini.
1983,
Pubmed
,
Echinobase
Green,
Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei.
1983,
Pubmed
Hentschel,
The organization and expression of histone gene families.
1981,
Pubmed
Hentschel,
Transcriptional fidelity of histone genes injected into Xenopus oocyte nuclei.
1980,
Pubmed
,
Echinobase
Kedes,
Histone genes and histone messengers.
1979,
Pubmed
Lerner,
Are snRNPs involved in splicing?
1980,
Pubmed
Mauron,
Monocistronic transcription is the physiological mechanism of sea urchin embryonic histone gene expression.
1981,
Pubmed
,
Echinobase
Montell,
Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3' consensus sequence AAUAAA.
1983,
Pubmed
Mount,
The U1 small nuclear RNA-protein complex selectively binds a 5' splice site in vitro.
1983,
Pubmed
Probst,
Expression of sea urchin histone genes in the oocyte of Xenopus laevis.
1979,
Pubmed
,
Echinobase
Queen,
Differential translation efficiency explains discoordinate expression of the galactose operon.
1981,
Pubmed
Remaut,
Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda.
1981,
Pubmed
Spinelli,
Transcription of sea-urchin mesenchyme blastula histone genes after heat shock.
1982,
Pubmed
,
Echinobase
Stunnenberg,
Bioassay for components regulating eukaryotic gene expression: a chromosomal factor involved in the generation of histone mRNA 3' termini.
1982,
Pubmed
,
Echinobase
Weaver,
Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates.
1979,
Pubmed