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Echinobase
ECB-ART-41628
J Cell Sci 2010 Jun 01;123Pt 11:1884-93. doi: 10.1242/jcs.066084.
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Membrane wounding triggers ATP release and dysferlin-mediated intercellular calcium signaling.

Covian-Nares JF , Koushik SV , Puhl HL , Vogel SS .


Abstract
Dysferlin is a Ca(2+)-binding protein found in many different cell types. It is required for membrane wound repair in muscle, but it is not known whether it has the same function in other cells. Here we report the activation of an intercellular signaling pathway in sea urchin embryos by membrane wounding that evokes Ca(2+) spikes in neighboring cells. This pathway was mimicked by ATP application, and inhibited by apyrase, cadmium, and omega-agatoxin-IVA. Microinjection of dysferlin antisense phosphorodiamidate morpholino oligonucleotides blocked this pathway, whereas control morpholinos did not. Co-injection of mRNA encoding human dysferlin with the inhibitory morpholino rescued signaling activity. We conclude that in sea urchin embryos dysferlin mediates Ca(2+)-triggered intercellular signaling in response to membrane wounding.

PubMed ID: 20442251
PMC ID: PMC2873225
Article link: J Cell Sci
Grant support: [+]

Genes referenced: LOC100887844 LOC115919910 otof
Morpholinos: LOC589501 MO2 LOC589501 MO3

References [+] :
Anselmi, ATP release through connexin hemichannels and gap junction transfer of second messengers propagate Ca2+ signals across the inner ear. 2008, Pubmed