Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
J Cell Biol
1983 Nov 01;975 Pt 1:1612-21. doi: 10.1083/jcb.97.5.1612.
Show Gene links
Show Anatomy links
An actin-depolymerizing protein (depactin) from starfish oocytes: properties and interaction with actin.
Mabuchi I
.
???displayArticle.abstract???
Physico-chemical properties and interaction with actin of an actin-depolymerizing protein from mature starfish oocytes were studied. This protein, which is called depactin, exists in a monomeric form under physiological conditions. Its molecular weight is approximately 20,000 for the native protein and approximately 17,000 for denatured protein. The Glu + Asp/Lys + Arg molar ratio of this protein is 1.55. The apparent pl of the denatured depactin is approximately 6. The extent of actin polymerization is reduced by the presence of depactin; however, the rate of polymerization seems to be accelerated as measured spectrophotometrically at 238nm. This effect is interpreted to indicate that depactin cut the newly formed filaments into small fragments, thereby increasing the number of the filament ends to which monomers are added. The apparent critical concentration of actin for polymerization, as determined by viscometry or flow birefringence measurement, is increased by the presence of depactin in a concentration-dependent manner. Raising the pH of the solution does not reverse the action of depactin. The molar ratio of actin and depactin, which interact with each other, is estimated to be 1:1 by means of a cross-linking experiment using a water-soluble carbodiimide. Depactin binds to a DNase I-Sepharose column via actin and is selectively eluted with 0.6 M KCl or 0.6 M Kl. The association constant between actin and depactin is estimated, using the column, to be 2-3 X 10(6) M-1. The content of depactin in the high-speed supernatant of the oocyte extract is determined to be 1%; this can act upon approximately 63% of the actin in the supernatant.
Bamburg,
Partial purification and characterization of an actin depolymerizing factor from brain.
1980, Pubmed
Bamburg,
Partial purification and characterization of an actin depolymerizing factor from brain.
1980,
Pubmed
Begg,
pH regulates the polymerization of actin in the sea urchin egg cortex.
1979,
Pubmed
,
Echinobase
Bernstein,
Tropomyosin binding to F-actin protects the F-actin from disassembly by brain actin-depolymerizing factor (ADF).
1982,
Pubmed
Blikstad,
Selective assay of monomeric and filamentous actin in cell extracts, using inhibition of deoxyribonuclease I.
1978,
Pubmed
Blikstad,
Isolation and characterization of profilactin and profilin from calf thymus and brain.
1980,
Pubmed
Borejdo,
Polymerization of G-actin by hydrodynamic shear stresses.
1981,
Pubmed
Bray,
Unpolymerized actin in fibroblasts and brain.
1976,
Pubmed
Bretscher,
Villin is a major protein of the microvillus cytoskeleton which binds both G and F actin in a calcium-dependent manner.
1980,
Pubmed
Brown,
Mechanism of action of cytochalasin: evidence that it binds to actin filament ends.
1981,
Pubmed
Burgess,
Polarized bundles of actin filaments within microvilli of fertilized sea urchin eggs.
1977,
Pubmed
,
Echinobase
Carlsson,
Actin polymerizability is influenced by profilin, a low molecular weight protein in non-muscle cells.
1977,
Pubmed
Craig,
Regulation of actin polymerization by villin, a 95,000 dalton cytoskeletal component of intestinal brush borders.
1980,
Pubmed
Glenney,
Calcium control of the intestinal microvillus cytoskeleton: its implications for the regulation of microfilament organizations.
1980,
Pubmed
Gordon,
Comparative biochemistry of non-muscle actins.
1977,
Pubmed
Greene,
The binding of heavy meromyosin to F-actin.
1980,
Pubmed
Harris,
An actin depolymerizing protein from pig plasma.
1981,
Pubmed
Hasegawa,
Fragmin: a calcium ion sensitive regulatory factor on the formation of actin filaments.
1980,
Pubmed
Hatano,
Some properties of Physarum actinin. A regulatory protein of actin polymerization.
1979,
Pubmed
,
Echinobase
Higashi,
Conformational changes associated with polymerization and nucleotide binding in actin molecules.
1965,
Pubmed
Hitchcock,
Depolymerization of F-actin by deoxyribonuclease I.
1976,
Pubmed
Isenberg,
An actin-binding protein from Acanthamoeba regulates actin filament polymerization and interactions.
1980,
Pubmed
Kanatani,
Induction of spawning and oocyte maturation by L-methyl-adenine in starfishes.
1969,
Pubmed
,
Echinobase
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lin,
Proteins and complexes that affect actin-filament assembly and interactions.
1982,
Pubmed
Lin,
Cytochalasins inhibit nuclei-induced actin polymerization by blocking filament elongation.
1980,
Pubmed
Lindberg,
DNase-I inhibitor protein. Isolation with DNase-agarose.
1974,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Mabuchi,
Effects of muscle proteins on the interaction between actin and an actin-depolymerizing protein from starfish oocytes.
1982,
Pubmed
,
Echinobase
Mabuchi,
Purification from starfish eggs of a protein that depolymerizes actin.
1981,
Pubmed
,
Echinobase
Mabuchi,
Purification and properties of soluble actin from sea urchin eggs.
1980,
Pubmed
,
Echinobase
MacLean-Fletcher,
Mechanism of action of cytochalasin B on actin.
1980,
Pubmed
MacLean-Fletcher,
Viscometric analysis of the gelation of Acanthamoeba extracts and purification of two gelation factors.
1980,
Pubmed
Mannherz,
A specific 1:1 G-actin:DNAase i complex formed by the action of DNAase I on F-actin.
1975,
Pubmed
Maruyama,
beta-actinin, a regulatory protein of muscle. Purification, characterization and function.
1977,
Pubmed
Maruyama,
Sonic vibration induces the nucleation of actin in the absence of magnesium ions and cytochalasins inhibit the elongation of the nuclei.
1981,
Pubmed
Merriam,
Actin in Xenopus oocytes. II. Intracellular distribution and polymerizability.
1978,
Pubmed
Nagata,
Changes in contractile proteins during differentiation of myeloid leukemia cells. I. Polymerization of actin.
1980,
Pubmed
Nishida,
A new protein factor that modulates both microtubule assembly and actin polymerization.
1981,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed
Ozaki,
Isolation and characterization of Physarum profilin.
1983,
Pubmed
Pardee,
Purification of muscle actin.
1982,
Pubmed
Pollard,
Actin structure, polymerization, and gelation.
1982,
Pubmed
Rees,
Studies on the isolation and molecular properties of homogeneous globular actin. Evidence for a single polypeptide chain structure.
1967,
Pubmed
Reichstein,
Acanthamoeba profilin. A protein of low molecular weight from Acanpthamoeba castellanii that inhibits actin nucleation.
1979,
Pubmed
Rich,
Detection of conformational changes in actin by proteolytic digestion: evidence for a new monomeric species.
1976,
Pubmed
Schroeder,
Microfilament-mediated surface change in starfish oocytes in response to 1-methyladenine: implications for identifying the pathway and receptor sites for maturation-inducing hormones.
1981,
Pubmed
,
Echinobase
Southwick,
Isolation of an inhibitor of actin polymerization from human polymorphonuclear leukocytes.
1981,
Pubmed
Spudich,
The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.
1971,
Pubmed
Spudich,
Control of assembly of Dictyostelium myosin and actin filaments.
1982,
Pubmed
Spudich,
Actin in triton-treated cortical preparations of unfertilized and fertilized sea urchin eggs.
1979,
Pubmed
,
Echinobase
Spudich,
Supramolecular forms of actin from amoebae of Dictyostelium discoideum.
1975,
Pubmed
Sugino,
Effect of fragmin on actin polymerization: evidence for enhancement of nucleation and capping of the barbed end.
1982,
Pubmed
Tilney,
The polymerization of actin: its role in the generation of the acrosomal process of certain echinoderm sperm.
1973,
Pubmed
,
Echinobase
Tobacman,
The regulation of actin polymerization and the inhibition of monomeric actin ATPase activity by Acanthamoeba profilin.
1982,
Pubmed
Tseng,
Mechanism of action of Acanthamoeba profilin: demonstration of actin species specificity and regulation by micromolar concentrations of MgCl2.
1982,
Pubmed
Van Baelen,
Vitamin D-binding protein (Gc-globulin) binds actin.
1980,
Pubmed
