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Terasaki M
,
Campagnola P
,
Rolls MM
,
Stein PA
,
Ellenberg J
,
Hinkle B
,
Slepchenko B
.
???displayArticle.abstract??? Nuclear envelope breakdown was investigated during meiotic maturation of starfish oocytes. Fluorescent 70-kDa dextran entry, as monitored by confocal microscopy, consists of two phases, a slow uniform increase and then a massive wave. From quantitative analysis of the first phase of dextran entry, and from imaging of green fluorescent protein chimeras, we conclude that nuclear pore disassembly begins several minutes before nuclear envelope breakdown. The best fit for the second phase of entry is with a spreading disruption of the membrane permeability barrier determined by three-dimensional computer simulations of diffusion. We propose a new model for the mechanism of nuclear envelope breakdown in which disassembly of the nuclear pores leads to a fenestration of the nuclear envelope double membrane.
Collas,
Sequential PKC- and Cdc2-mediated phosphorylation events elicit zebrafish nuclear envelope disassembly.
1999,
Pubmed
Ellenberg,
Nuclear membrane dynamics and reassembly in living cells: targeting of an inner nuclear membrane protein in interphase and mitosis.
1997,
Pubmed
Favreau,
Cell cycle-dependent phosphorylation of nucleoporins and nuclear pore membrane protein Gp210.
1996,
Pubmed
Gallant,
Cyclin B2 undergoes cell cycle-dependent nuclear translocation and, when expressed as a non-destructible mutant, causes mitotic arrest in HeLa cells.
1992,
Pubmed
Gerace,
The nuclear envelope lamina is reversibly depolymerized during mitosis.
1980,
Pubmed
HIRAMOTO,
Microinjection of the live spermatozoa into sea urchin eggs.
1962,
Pubmed
,
Echinobase
Heald,
Mutations of phosphorylation sites in lamin A that prevent nuclear lamina disassembly in mitosis.
1990,
Pubmed
Kanatani,
Isolation and indentification on meiosis inducing substance in starfish Asterias amurensis.
1969,
Pubmed
,
Echinobase
Lee,
C. elegans nuclear envelope proteins emerin, MAN1, lamin, and nucleoporins reveal unique timing of nuclear envelope breakdown during mitosis.
2000,
Pubmed
Luby-Phelps,
Probing the structure of cytoplasm.
1986,
Pubmed
Macaulay,
Differential mitotic phosphorylation of proteins of the nuclear pore complex.
1995,
Pubmed
Newport,
Disassembly of the nucleus in mitotic extracts: membrane vesicularization, lamin disassembly, and chromosome condensation are independent processes.
1987,
Pubmed
Ookata,
Relocation and distinct subcellular localization of p34cdc2-cyclin B complex at meiosis reinitiation in starfish oocytes.
1992,
Pubmed
,
Echinobase
Otto,
Microtubule arrays in the cortex and near the germinal vesicle of immature starfish oocytes.
1984,
Pubmed
,
Echinobase
Peter,
In vitro disassembly of the nuclear lamina and M phase-specific phosphorylation of lamins by cdc2 kinase.
1990,
Pubmed
Peters,
Nuclear envelope permeability measured by fluorescence microphotolysis of single liver cell nuclei.
1983,
Pubmed
Peters,
Nucleo-cytoplasmic flux and intracellular mobility in single hepatocytes measured by fluorescence microphotolysis.
1984,
Pubmed
Pérez-Mongiovi,
A propagated wave of MPF activation accompanies surface contraction waves at first mitosis in Xenopus.
1998,
Pubmed
Rolls,
A visual screen of a GFP-fusion library identifies a new type of nuclear envelope membrane protein.
1999,
Pubmed
Rout,
The yeast nuclear pore complex: composition, architecture, and transport mechanism.
2000,
Pubmed
Schaff,
A general computational framework for modeling cellular structure and function.
1997,
Pubmed
Stafstrom,
Dynamics of the nuclear envelope and of nuclear pore complexes during mitosis in the Drosophila embryo.
1984,
Pubmed
Terasaki,
Dynamics of the endoplasmic reticulum and golgi apparatus during early sea urchin development.
2000,
Pubmed
,
Echinobase
Terasaki,
Structural change of the endoplasmic reticulum during fertilization: evidence for loss of membrane continuity using the green fluorescent protein.
1996,
Pubmed
,
Echinobase
Terasaki,
Redistribution of cytoplasmic components during germinal vesicle breakdown in starfish oocytes.
1994,
Pubmed
,
Echinobase
Ward,
Identification of cell cycle-regulated phosphorylation sites on nuclear lamin C.
1990,
Pubmed
Yang,
Integral membrane proteins of the nuclear envelope are dispersed throughout the endoplasmic reticulum during mitosis.
1997,
Pubmed
Zaal,
Golgi membranes are absorbed into and reemerge from the ER during mitosis.
1999,
Pubmed
