ECB-ART-52206
Dev Growth Differ
1972 Nov 01;143:247-261. doi: 10.1111/j.1440-169X.1972.00247.x.
Show Gene links
Show Anatomy links
CORTEX PROTEIN OF SEA URCHIN EGGS I. ITS PURIFICATION AND OTHER PROTEIN COMPONENTS OF THE CORTEX.
???displayArticle.abstract???
Isolated cortical hull of the sea urchin egg consisted of a gel layer having 3-4 μ in thickness which could be dispersed with 0.6 m KCl. After removing a protein fraction soluble in 10 mm Tris-HCl buffer (pH 7.0-7.2) containing 1 mm ATP or EDTA and 1 mm GSH, so called KCl-soluble protein of the cortices was obtained. After purifying the "cortex protein", it was homogeneous so far as checked by ultracentrifugation and electrophoresis on a polyacrylamide gel. The cortex protein had a thiol-disulfide exchange activity to Ca-insoluble protein in the ATP-extract of the cortices catalyzed by a transhydrogenase. Neither ovoactin nor actomyosin-like protein was detected in the ATP-extract or the 0.6 m KCl-extract of the cortices respectively. Hyalin was not detected in our KCl-soluble protein fractions of isolated cortices.
???displayArticle.pubmedLink??? 37280854
???displayArticle.link??? Dev Growth Differ