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Echinobase
ECB-ART-32066
Arch Environ Contam Toxicol 1989 Sep 01;185:748-55. doi: 10.1007/bf01225012.
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Comparative sensitivity of sea urchin sperm bioassays to metals and pesticides.

Dinnel PA , Link JM , Stober QJ , Letourneau MW , Roberts WE .


Abstract
A simple sperm/fertilization bioassay, primarily using sea urchin gametes, has been developed and used by a variety of laboratories. This assay was recently refined into a standard test and is now being used by the U.S. Environmental Protection Agency and others for toxicity testing in marine waters. One factor that has lagged behind the development of this assay is the comparison of its sensitivity to various common toxicants as compared to other bioassay systems and life stages of other marine organisms. The objective of this study was to compare the sensitivity of a standardized sea urchin sperm/fertilization assay to the responses of embryo, larval, and adult marine organisms to metals (Ag, Cd, Cu, Pb, Zn) and pesticides (DDT, Dieldrin, Endrin, Endosulfan) added to natural seawater. The results, although highly variable, generally showed that sperm/fertilization and embryo assays were quite sensitive to the metals tested, but that the larval and adult assays were more sensitive to the pesticides. These comparative data, together with other studies of complex effluents, show that the standardized sperm/fertilization bioassay is an especially quick and useful tool for biomonitoring of marine waters.

PubMed ID: 2802677
Article link: Arch Environ Contam Toxicol


Genes referenced: LOC100887844

References [+] :
BARRON, Regulatory mechanisms of cellular respiration; the role of soluble sulfhydryl groups as shown by the effect of sulfhydryl reagents on the respiration of sea urchin sperm. 1948, Pubmed, Echinobase