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J Cell Biol
1985 Mar 01;1003:794-9. doi: 10.1083/jcb.100.3.794.
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Interaction of the sperm adhesive protein, bindin, with phospholipid vesicles. I. Specific association of bindin with gel-phase phospholipid vesicles.
Glabe CG
.
???displayArticle.abstract??? Bindin is a 30,000-mol-wt protein of sea urchin sperm that is responsible for the specific adhesion of the sperm acrosomal process to the vitelline layer covering the egg plasma membrane during fertilization. Sulfated glycoconjugates are believed to be the egg surface receptors for bindin, but the mechanism by which bindin associates with the sperm acrosomal membrane is unknown. Here I report that bindin specifically associates with phospholipid vesicles in vitro. Interaction of the bindin polypeptide with liposomes was found to cause an increase in the density of the liposomes and induce the aggregation of the vesicles. A novel property of this association of bindin with membranes was that it required phospholipids in a gel phase. The interaction of bindin with liposomes was greatly reduced at temperatures above the phase transition temperature. The interaction of bindin with gel-phase vesicles appeared to be reversible, since the aggregated vesicles dissaggregated as the temperature was raised above the phase transition temperature. Association of bindin with the bilayer did not alter the accessibility of the polypeptide to cleavage by trypsin, which suggests that most of the polypeptide chain remains exposed at the surface of the membrane.
Bearer,
Modifications of anionic-lipid domains preceding membrane fusion in guinea pig sperm.
1982, Pubmed
Bearer,
Modifications of anionic-lipid domains preceding membrane fusion in guinea pig sperm.
1982,
Pubmed
Caron,
Interaction of microtubule proteins with phospholipid vesicles.
1979,
Pubmed
Collins,
A reevaluation of the fertilizin hypothesis of sperm agglutination and the description of a novel form of sperm adhesion.
1976,
Pubmed
,
Echinobase
Fraker,
Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril.
1978,
Pubmed
Glabe,
Interaction of the sperm adhesive protein, bindin, with phospholipid vesicles. II. Bindin induces the fusion of mixed-phase vesicles that contain phosphatidylcholine and phosphatidylserine in vitro.
1985,
Pubmed
,
Echinobase
Glabe,
Species specific agglutination of eggs by bindin isolated from sea urchin sperm.
1977,
Pubmed
,
Echinobase
Glabe,
Species-specific sperm adhesion in sea urchins. A quantitative investigation of bindin-mediated egg agglutination.
1979,
Pubmed
,
Echinobase
Glabe,
Isolation of a high molecular weight glycoconjugate derived from the surface of S purpuratus eggs that is implicated in sperm adhesion.
1981,
Pubmed
,
Echinobase
Glabe,
Carbohydrate specificity of sea urchin sperm bindin: a cell surface lectin mediating sperm-egg adhesion.
1982,
Pubmed
,
Echinobase
Klausner,
Selectivity of fluorescent lipid analogues for lipid domains.
1980,
Pubmed
Klausner,
Interaction of tubulin with phospholipid vesicles. I. Association with vesicles at the phase transition.
1981,
Pubmed
Kumar,
Interaction of tubulin with phospholipid vesicles. II. Physical changes of the protein.
1981,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Morrisett,
Lipid-protein interactions in the plasma lipoproteins.
1977,
Pubmed
Moy,
Immunoperoxidase localization of bindin during the adhesion of sperm to sea urchin eggs.
1979,
Pubmed
,
Echinobase
Müller-Eberhard,
Complement.
1975,
Pubmed
Rossignol,
Sperm-egg binding: identification of a species-specific sperm receptor from eggs of Stronglyocentrotus purpuratus.
1981,
Pubmed
,
Echinobase
Shimshick,
Lateral phase separation in phospholipid membranes.
1973,
Pubmed
Vacquier,
Isolation of bindin: the protein responsible for adhesion of sperm to sea urchin eggs.
1977,
Pubmed
,
Echinobase
Vacquier,
Purification of sea urchin sperm bindin by DEAE-cellulose chromatography.
1983,
Pubmed
,
Echinobase
White,
Membrane fusion proteins of enveloped animal viruses.
1983,
Pubmed
Wickner,
Role of hydrophobic forces in membrane protein asymmetry.
1977,
Pubmed
Wolf,
Changes in the organization of the sea urchin egg plasma membrane upon fertilization: indications from the lateral diffusion rates of lipid-soluble fluorescent dyes.
1981,
Pubmed
,
Echinobase
van der Bosch,
Fusion of dipalmitoylphosphatidylcholine vesicle membranes induced by concanavalin A.
1975,
Pubmed
