Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mol Biol Cell
2003 Feb 01;142:774-85. doi: 10.1091/mbc.02-06-0089.
Show Gene links
Show Anatomy links
Identification of a novel leucine-rich repeat protein as a component of flagellar radial spoke in the Ascidian Ciona intestinalis.
Padma P
,
Satouh Y
,
Wakabayashi K
,
Hozumi A
,
Ushimaru Y
,
Kamiya R
,
Inaba K
.
???displayArticle.abstract???
Axonemes are highly organized microtubule-based structures conserved in many eukaryotes. In an attempt to study axonemes by a proteomics approach, we selectively cloned cDNAs of axonemal proteins by immunoscreening the testis cDNA library from the ascidian Ciona intestinalis by using an antiserum against whole axonemes. We report here a 37-kDa protein of which cDNA occurred most frequently among total positive clones. This protein, named LRR37, belongs to the class of SDS22+ leucine-rich repeat (LRR) family. LRR37 is different from the LRR outer arm dynein light chain reported in Chlamydomonas and sea urchin flagella, and thus represents a novel axonemalLRR protein. Immunoelectron microscopy by using a polyclonal antibody against LRR37 showed that it is localized on the tip of the radial spoke, most likely on the spoke head. The LRR37 protein in fact seems to form a complex together with radial spoke protein 3 in a KI extract of the axonemes. These results suggest that LRR37 is a component of the radial spoke head and is involved in the interaction with other radial spoke components or proteins in the central pair projection.
Ansari-Lari,
Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6.
1998, Pubmed
Ansari-Lari,
Comparative sequence analysis of a gene-rich cluster at human chromosome 12p13 and its syntenic region in mouse chromosome 6.
1998,
Pubmed
Benashski,
Light chain 1 from the Chlamydomonas outer dynein arm is a leucine-rich repeat protein associated with the motor domain of the gamma heavy chain.
1999,
Pubmed
Blouin,
Primary ciliary dyskinesia: a genome-wide linkage analysis reveals extensive locus heterogeneity.
2000,
Pubmed
Buchanan,
Structural and functional diversity in the leucine-rich repeat family of proteins.
1996,
Pubmed
Chaudhry,
Multiple protein kinase activities required for activation of sperm flagellar motility.
1995,
Pubmed
Cowan,
Disorders of ciliary motility.
2001,
Pubmed
Curry,
Flagellar radial spoke: a model molecular genetic system for studying organelle assembly.
1993,
Pubmed
Diener,
Assembly of flagellar radial spoke proteins in Chlamydomonas: identification of the axoneme binding domain of radial spoke protein 3.
1993,
Pubmed
Earnshaw,
Molecular cloning of cDNA for CENP-B, the major human centromere autoantigen.
1987,
Pubmed
Gaillard,
Flagellar radial spoke protein 3 is an A-kinase anchoring protein (AKAP).
2001,
Pubmed
Gibbons,
Cilia and flagella of eukaryotes.
1981,
Pubmed
Gingras,
Molecular cloning and characterization of a radial spoke head protein of sea urchin sperm axonemes: involvement of the protein in the regulation of sperm motility.
1998,
Pubmed
,
Echinobase
Goodenough,
Substructure of inner dynein arms, radial spokes, and the central pair/projection complex of cilia and flagella.
1985,
Pubmed
,
Echinobase
Hamasaki,
In vitro phosphorylation of Paramecium axonemes and permeabilized cells.
1989,
Pubmed
Hasegawa,
Stimulation of in vitro motility of Chlamydomonas axonemes by inhibition of cAMP-dependent phosphorylation.
1987,
Pubmed
Howard,
Regulation of Chlamydomonas flagellar dynein by an axonemal protein kinase.
1994,
Pubmed
Huang,
Radial spokes of Chlamydomonas flagella: genetic analysis of assembly and function.
1981,
Pubmed
Huang,
Suppressor mutations in Chlamydomonas reveal a regulatory mechanism for Flagellar function.
1982,
Pubmed
Inaba,
Tctex2-related outer arm dynein light chain is phosphorylated at activation of sperm motility.
1999,
Pubmed
,
Echinobase
Inaba,
Purification of proteasomes from salmonid fish sperm and their localization along sperm flagella.
1993,
Pubmed
Inaba,
Proteasomes regulate the motility of salmonid fish sperm through modulation of cAMP-dependent phosphorylation of an outer arm dynein light chain.
1998,
Pubmed
Inaba,
EST analysis of gene expression in testis of the ascidian Ciona intestinalis.
2002,
Pubmed
Kajava,
Structural diversity of leucine-rich repeat proteins.
1998,
Pubmed
Kobe,
The leucine-rich repeat: a versatile binding motif.
1994,
Pubmed
Kobe,
A structural basis of the interactions between leucine-rich repeats and protein ligands.
1995,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lapeyre,
Nucleolin, the major nucleolar protein of growing eukaryotic cells: an unusual protein structure revealed by the nucleotide sequence.
1987,
Pubmed
Nonaka,
Randomization of left-right asymmetry due to loss of nodal cilia generating leftward flow of extraembryonic fluid in mice lacking KIF3B motor protein.
1998,
Pubmed
Ohkura,
S. pombe gene sds22+ essential for a midmitotic transition encodes a leucine-rich repeat protein that positively modulates protein phosphatase-1.
1991,
Pubmed
Padma,
Molecular cloning and characterization of a thioredoxin/nucleoside diphosphate kinase related dynein intermediate chain from the ascidian, Ciona intestinalis.
2001,
Pubmed
,
Echinobase
Pazour,
Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene tg737, are required for assembly of cilia and flagella.
2000,
Pubmed
Piperno,
Radial spokes of Chlamydomonas flagella: polypeptide composition and phosphorylation of stalk components.
1981,
Pubmed
Porter,
The 9 + 2 axoneme anchors multiple inner arm dyneins and a network of kinases and phosphatases that control motility.
2000,
Pubmed
San Agustin,
Role of cAMP in the reactivation of demembranated ram spermatozoa.
1994,
Pubmed
Schulman,
Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex.
2000,
Pubmed
Smith,
Regulation of flagellar dynein by the axonemal central apparatus.
2002,
Pubmed
Smith,
Regulation of dynein-driven microtubule sliding by the radial spokes in flagella.
1992,
Pubmed
Warner,
The structural basis of ciliary bend formation. Radial spoke positional changes accompanying microtubule sliding.
1974,
Pubmed
Witman,
Chlamydomonas flagellar mutants lacking radial spokes and central tubules. Structure, composition, and function of specific axonemal components.
1978,
Pubmed
Xue,
Identification of a novel testis-specific leucine-rich protein in humans and mice.
2000,
Pubmed
Yang,
Protein phosphatases PP1 and PP2A are located in distinct positions in the Chlamydomonas flagellar axoneme.
2000,
Pubmed
Yang,
Localization of calmodulin and dynein light chain LC8 in flagellar radial spokes.
2001,
Pubmed
