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Chromosoma
1978 Dec 06;693:291-306. doi: 10.1007/bf00332133.
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Histone genes in macronuclear DNA of the ciliate Stylonychia mytilus.
Elsevier SM
,
Lipps HJ
,
Steinbrück G
.
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DNA in the macronucleus of Stylonychia mytilus exists as discrete gene-sized fragments which are derived from micronuclear DNA through a series of well-defined developmental events. It has been proposed that each of the DNA fragments might represent a gene and its controlling elements. We have investigated this possibility using genes which code for the five histone proteins. Macronuclear DNA fragments were fractionated according to size by agarose gel electrophoresis, the fragments transferred to nitrocellulose filters using the technique of Southern, and the filter-bound DNA hybridized with labeled cloned histone genes of the sea urchin, Psammechinus miliaris. Results indicate, first, that sequences homologous to the five individual histone gene probes are present in discrete macronuclear fragments which appear as bands in the gel hybridization assay. Secondly, for each of the five individual histone gene probes the homologous DNA fragments are several in number, ranging in size in from 7.6 Kb (Kilo base pairs) to 0.73 Kb. For example, the largest of six detected fragments hybridizing to the H3 gene probe contains approximately 10 times the amount of DNA required to code for a Stylonychia H3 histone. The smallest detected fragment hybridizing to the H3 probe contains enought DNA to code for approximately two copies of the histone. Finally, in general, no two histone approximately two copies of the histone. Finally, in general, no two histone gene probes hybridized to the same macronuclear DNA fragment. This result indicates that genes coding for the five histones in Stylonychia are not located together on the same macronuclear DNA fragments and implies that the five functionally related genes would not be transcribed together as a polycistronic unit.
Ammermann,
The development of the macronucleus in the ciliated protozoan Stylonychia mytilus.
1974, Pubmed
Ammermann,
The development of the macronucleus in the ciliated protozoan Stylonychia mytilus.
1974,
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Ammermann,
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Arceci,
The programmed switch in lysine-rich histone synthesis at gastrulation.
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Birnstiel,
Isolation and some properties of the genes coding for histone proteins.
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Birnstiel,
DNA sequences coding for the H2B histone of Psammechinus miliaris.
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Clarkson,
Integration of eukaryotic genes for 5S RNA and histone proteins into a phage lambda receptor.
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Cohen,
Stage-specific switches in histone synthesis during embryogenesis of the sea urchin.
1975,
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Cohn,
Histone genes of the sea urchin (S. purpuratus) cloned in E coli: order, polarity, and strandedness of the five histone-coding and spacer regions.
1976,
Pubmed
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Elgin,
Chromosomal proteins and chromatin structure.
1975,
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Gilbert,
Why genes in pieces?
1978,
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Gross,
Molecular analysis of the histone gene cluster of Psammechinus miliaris: I. Fractionation and identification of five individual histone mRNAs.
1976,
Pubmed
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Gross,
Molecular analysis of the histone gene cluster of Psammechinus miliaris: III. Polarity and asymmetry of the histone-coding sequences.
1976,
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Kedes,
Reiteration and clustering of DNA sequences complementary to histone messenger RNA.
1971,
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1975,
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Kloetzel,
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Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
1975,
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Lauth,
DNA of ciliated protozoa: DNA sequence diminution during macronuclear development of Oxytricha.
1976,
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Lawn,
Gene-sized DNA molecules of the Oxytricha macronucleus have the same terminal sequence.
1977,
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Lipps,
Free genes for rRNAs in the macronuclear genome of the ciliate Stylonychia mytilus.
1978,
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Melli,
Presence of histone mRNA sequences in high molecular weight RNA of HeLa cells.
1977,
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Murray,
Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli.
1975,
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Pardue,
Localization of sequences coding for histone messenger RNA in the chromosomes of Drosophila melanogaster.
1977,
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Portmann,
Partial denaturation mapping of cloned histone DNA from the sea urchin Psammechinus miliaris.
1976,
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Rigby,
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
1977,
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Schaffner,
Molecular analysis of the histone gene cluster of psammechinus miliaris: II. The arrangement of the five histone-coding and spacer sequences.
1976,
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Schaffner,
Genes and spacers of cloned sea urchin histone DNA analyzed by sequencing.
1978,
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Smith,
A simple method for DNA restriction site mapping.
1976,
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Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
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Spear,
Polytene chromosomes of Oxytricha: biochemical and morphological changes during macronuclear development in a ciliated protozoan.
1976,
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Weinberg,
Histone gene arrangement in the sea urchin, Strongylocentrotus purpuratus.
1975,
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Wesley,
Inverted repetitious sequences in the macronuclear DNA of hypotrichous ciliates.
1975,
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Yao,
A single integrated gene for ribosomal RNA in a eucaryote, Tetrahymena pyriformis.
1977,
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