ECB-ART-43599
Cold Spring Harb Protoc
2014 Sep 02;20149:988-92. doi: 10.1101/pdb.prot076901.
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Preparation and use of sea urchin egg homogenates for studying NAADP-mediated Ca²⁺ release.
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NAADP and other Ca(2+)-mobilizing messengers are membrane impermeant and thus must be added directly to cell-free or broken-cell preparations to effect Ca(2+) release. The sea urchin egg homogenate, where the biological activity of NAADP was first reported, remains the gold standard cell-free system for studying NAADP-mediated Ca(2+) release. Here we describe how to prepare sea urchin egg homogenate and use it to measure NAADP-mediated Ca(2+) release.
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Genes referenced: LOC100887844 LOC115919910