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Oxid Med Cell Longev
2016 Jan 01;2016:9251064. doi: 10.1155/2016/9251064.
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Morphological and Biochemical Effects on the Skeletal Muscle of Ovariectomized Old Female Rats Submitted to the Intake of Diets with Vegetable or Animal Protein and Resistance Training.
Figueiredo Braggion G
,
Ornelas E
,
Carmona Sattin Cury J
,
Edviges Alves Lima N
,
Aquino RC
,
Affonso Fonseca FL
,
Maifrino LB
.
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Introduction. Sarcopenia is a process characterized by reduction in protein mass and muscle strength with increasing age, especially in the postmenopausal period, resulting in functional limitations and with great impact on the physical autonomy of the elderly. Objective. To evaluate the effects of diets with vegetable proteins (VP) or animal proteins (AP) associated with resistance training (RT) on the structural and biochemical parameters of the medial gastrocnemius muscle in Wistar rats with sarcopenia. Methods. An experimental model with ovariectomized rats was used to induce sarcopenia and resistance training. The histochemical technique was used for the typing of muscle fibers, the cross-sectional area of myocytes, and volume densities of myocytes and interstitium; the technique of Picrosirius stain was used to highlight the collagen fibers. Results. The VP diet was not able to minimize the effects of sarcopenia in the medial gastrocnemius of sedentary animals and when associated with RT, it promoted maintenance of the CSA, attenuating the atrophy of type IIB fibers in the medial gastrocnemius. The AP diet in sedentary animals protected the type I fibers. When combined with RT, the AP promoted muscle remodeling, with reduction in volume density of type I and IIA fibers, and increase of IIB fibers, together with an increase in collagen volume density. Conclusion. The data suggest a tendency to better results of hypertrophy in animal groups that consumed the AP diet, even the sedentary animals, although more evident in those trained.
Figure 1. Average variation of food consumption (in grams of chow/animal/day), during the 12 weeks of intervention. Groups CVS, VOS, AOS, CAS, VOT, and AOT.
Figure 2. Cross-sectional images of the medial gastrocnemius muscle stained with the NADH-tr technique. CVS: sedentary, control, vegetable protein, VOS: sedentary, ovariectomized, vegetable protein, VOT: trained, ovariectomized, vegetable protein, CAS: sedentary, control, animal protein, AOS: sedentary, ovariectomized, animal protein, and AOT: trained, ovariectomized, animal protein. Arrows indicate the different types of fiber. Red: type I fibers, yellow: type IIA fibers, and green: type IIB fibers. Black arrows indicate interstitium.
Figure 3. Cross-sectional images of the medial gastrocnemius muscle stained with the Picrosirius technique, showing the collagen fibers according to groups. CVS: sedentary, control, vegetable protein, VOS: sedentary, ovariectomized, vegetable protein, VOT: trained, ovariectomized, vegetable protein, CAS: sedentary, control, animal protein, AOS: sedentary, ovariectomized, animal protein, and AOT: trained, ovariectomized, animal protein. Arrows indicate collagen fibers stained with the Picrosirius method.
American Dietetic Association,
Position of the American Dietetic Association and Dietitians of Canada: Vegetarian diets.
2003, Pubmed
American Dietetic Association,
Position of the American Dietetic Association and Dietitians of Canada: Vegetarian diets.
2003,
Pubmed
Arnold,
Skeletal diseases caused by mutations that affect collagen structure and function.
2013,
Pubmed
Asikainen,
Exercise for health for early postmenopausal women: a systematic review of randomised controlled trials.
2004,
Pubmed
Barberi,
Age-dependent alteration in muscle regeneration: the critical role of tissue niche.
2013,
Pubmed
Barnard,
Histochemical, biochemical, and contractile properties of red, white, and intermediate fibers.
1971,
Pubmed
Baumgartner,
Epidemiology of sarcopenia among the elderly in New Mexico.
1998,
Pubmed
Borg,
Morphology of connective tissue in skeletal muscle.
1980,
Pubmed
Brooke,
Muscle fiber types: how many and what kind?
1970,
Pubmed
Brooks,
Contractile properties of skeletal muscles from young, adult and aged mice.
1988,
Pubmed
Brooks,
Forces and powers of slow and fast skeletal muscles in mice during repeated contractions.
1991,
Pubmed
Børsheim,
Effect of amino acid supplementation on muscle mass, strength and physical function in elderly.
2008,
Pubmed
Campbell,
Synergistic use of higher-protein diets or nutritional supplements with resistance training to counter sarcopenia.
2007,
Pubmed
Carosio,
Impact of ageing on muscle cell regeneration.
2011,
Pubmed
Catalano,
Increased susceptibility to insulin resistance associated with abdominal obesity in aging rats.
2005,
Pubmed
Chalé,
Efficacy of whey protein supplementation on resistance exercise-induced changes in lean mass, muscle strength, and physical function in mobility-limited older adults.
2013,
Pubmed
Chevalier,
Whole-body protein anabolic response is resistant to the action of insulin in obese women.
2005,
Pubmed
Cruz-Jentoft,
Sarcopenia: European consensus on definition and diagnosis: Report of the European Working Group on Sarcopenia in Older People.
2010,
Pubmed
Cuthbertson,
Anabolic signaling deficits underlie amino acid resistance of wasting, aging muscle.
2005,
Pubmed
De Vries,
Physiological effects of an exercise training regimen upon men aged 52 to 88.
1970,
Pubmed
Dirks,
Apoptosis in skeletal muscle with aging.
2002,
Pubmed
Ferrando,
EAA supplementation to increase nitrogen intake improves muscle function during bed rest in the elderly.
2010,
Pubmed
Fujita,
Nutrient signalling in the regulation of human muscle protein synthesis.
2007,
Pubmed
Fujita,
Aerobic exercise overcomes the age-related insulin resistance of muscle protein metabolism by improving endothelial function and Akt/mammalian target of rapamycin signaling.
2007,
Pubmed
Graf,
Effects of whey protein supplements on metabolism: evidence from human intervention studies.
2011,
Pubmed
Guillet,
Impaired anabolic response of muscle protein synthesis is associated with S6K1 dysregulation in elderly humans.
2004,
Pubmed
Hamrick,
Age-related loss of muscle mass and bone strength in mice is associated with a decline in physical activity and serum leptin.
2006,
Pubmed
Hartman,
Consumption of fat-free fluid milk after resistance exercise promotes greater lean mass accretion than does consumption of soy or carbohydrate in young, novice, male weightlifters.
2007,
Pubmed
Janssen,
Low relative skeletal muscle mass (sarcopenia) in older persons is associated with functional impairment and physical disability.
2002,
Pubmed
Junqueira,
Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections.
1979,
Pubmed
Kim,
β-hydroxy-β-methylbutyrate did not enhance high intensity resistance training-induced improvements in myofiber dimensions and myogenic capacity in aged female rats.
2012,
Pubmed
Lee,
Viral expression of insulin-like growth factor-I enhances muscle hypertrophy in resistance-trained rats.
2004,
Pubmed
Lipman,
Pathologic characterization of brown Norway, brown Norway x Fischer 344, and Fischer 344 x brown Norway rats with relation to age.
1996,
Pubmed
Lushaj,
Sarcopenia accelerates at advanced ages in Fisher 344xBrown Norway rats.
2008,
Pubmed
Maltais,
Changes in muscle mass and strength after menopause.
2009,
Pubmed
Mandarim-de-Lacerda,
Stereological tools in biomedical research.
2003,
Pubmed
Marzetti,
Mitochondrial death effectors: relevance to sarcopenia and disuse muscle atrophy.
2010,
Pubmed
Moreira,
[Skeletal muscle histomorphometric study of rats in anestrus].
2005,
Pubmed
Paddon-Jones,
Dietary protein recommendations and the prevention of sarcopenia.
2009,
Pubmed
Tang,
Ingestion of whey hydrolysate, casein, or soy protein isolate: effects on mixed muscle protein synthesis at rest and following resistance exercise in young men.
2009,
Pubmed
Visser,
Muscle mass, muscle strength, and muscle fat infiltration as predictors of incident mobility limitations in well-functioning older persons.
2005,
Pubmed
Volpi,
The response of muscle protein anabolism to combined hyperaminoacidemia and glucose-induced hyperinsulinemia is impaired in the elderly.
2000,
Pubmed
Westerterp-Plantenga,
Dietary protein, weight loss, and weight maintenance.
2009,
Pubmed
Wolfe,
Regulation of muscle protein by amino acids.
2002,
Pubmed
Zuliani,
Nutritional parameters, body composition, and progression of disability in older disabled residents living in nursing homes.
2001,
Pubmed