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Proc Natl Acad Sci U S A
1996 Jun 25;9313:6808-13. doi: 10.1073/pnas.93.13.6808.
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Molecular cloning of the first metazoan beta-1,3 glucanase from eggs of the sea urchin Strongylocentrotus purpuratus.
Bachman ES
,
McClay DR
.
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We report the molecular cloning of the first beta-1,3 glucanase from animal tissue. Three peptide sequences were obtained from beta-1,3 glucanase that had been purified from eggs of the sea urchin Strongylocentrotus purpuratus and the gene was cloned by PCR using oligonucleotides deduced from the peptide sequences. The full-length cDNA shows a predicted enzyme structure of 499 aa with a hydrophobic signal sequence. A 3.2-kb message is present in eggs, during early embryogenesis, and in adult gut tissue. A polyclonal antibody to the native 68-kDa enzyme recognizes a single band during early embryogenesis that reappears in the adult gut, and recognizes a 57-kDa fusion protein made from a full-length cDNA clone for beta-1,3 glucanase. The identity of this molecule as beta-1,3 glucanase is confirmed by sequence homology, by the presence of all three peptide sequences in the deduced amino acid sequence, and by the recognition of the bacterial fusion protein by the antibody directed against the native enzyme. Data base searches show significant homology at the amino acid level to beta-1,3 glucanases from two species of bacteria and a clotting factor from the horseshoe crab. The homology with the bacteria is centered in a 304-aa region in which there are seven scattered regions of high homology between the four divergent species. These four species were also found to have two homologous regions in common with more distantly related plant, fungal, and bacterial proteins. A global phylogeny based on these regions strongly suggests that the glucanases are a very ancient family of genes. In particular, there is an especially deep split within genes taken from the bacterial genus Bacillus.
Altschul,
Basic local alignment search tool.
1990, Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Epel,
Beta-1,3-glucanase of sea urchin eggs: release from particles at fertilization.
1969,
Pubmed
,
Echinobase
Foltz,
Sea urchin egg receptor for sperm: sequence similarity of binding domain and hsp70.
1993,
Pubmed
,
Echinobase
Henrissat,
A classification of glycosyl hydrolases based on amino acid sequence similarities.
1991,
Pubmed
Higgins,
CLUSTAL V: improved software for multiple sequence alignment.
1992,
Pubmed
Keitel,
Molecular and active-site structure of a Bacillus 1,3-1,4-beta-glucanase.
1993,
Pubmed
Kollár,
Architecture of the yeast cell wall. The linkage between chitin and beta(1-->3)-glucan.
1995,
Pubmed
Kozak,
Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
1986,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Mauch,
Antifungal Hydrolases in Pea Tissue : II. Inhibition of Fungal Growth by Combinations of Chitinase and beta-1,3-Glucanase.
1988,
Pubmed
Molina,
Purification and some properties of Candida albicans exo-1,3-beta-glucanase.
1989,
Pubmed
Planas,
Essential catalytic role of Glu134 in endo-beta-1,3-1,4-D-glucan 4-glucanohydrolase from B. licheniformis as determined by site-directed mutagenesis.
1992,
Pubmed
Rosenthal,
Sequence analysis of translationally controlled maternal mRNAs from Urechis caupo.
1993,
Pubmed
Sakellaris,
Genes from Cellvibrio mixtus encoding beta-1,3 endoglucanase.
1990,
Pubmed
Simmons,
Structure of a rice beta-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors.
1992,
Pubmed
Spilliaert,
Cloning and sequencing of a Rhodothermus marinus gene, bglA, coding for a thermostable beta-glucanase and its expression in Escherichia coli.
1994,
Pubmed
Strathmann,
Transposon-facilitated DNA sequencing.
1991,
Pubmed
Talbot,
The purification and characterization of an exo-beta (1 going to 3)-glucanohydrolase from sea urchin eggs.
1982,
Pubmed
,
Echinobase
Turkiewicz,
An endo-(1----3)-beta-glucanase and a collagenolytic serine proteinase from Euphausia superba Dana (Antarctic krill).
1991,
Pubmed
Vacquier,
The appearance of beta-1,3-glucanase in hatching embryos of the sea urchin, Echinometra vanbrunti.
1975,
Pubmed
,
Echinobase
Ward,
Differential Regulation of beta-1,3-Glucanase Messenger RNAs in Response to Pathogen Infection.
1991,
Pubmed
Watanabe,
Three N-terminal domains of beta-1,3-glucanase A1 are involved in binding to insoluble beta-1,3-glucan.
1992,
Pubmed
Wessel,
A cortical granule-specific enzyme, B-1,3-glucanase, in sea urchin eggs.
1987,
Pubmed
,
Echinobase
