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Mol Gen Genet
1996 Jun 24;2514:397-406. doi: 10.1007/BF02172367.
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A gene for maackiain detoxification from a dispensable chromosome of Nectria haematococca.
Covert SF
,
Enkerli J
,
Miao VP
,
VanEtten HD
.
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In Nectria haematococca the MAK1 gene product converts a chick-pea (Cicer arietinum) phytoalexin, maackiain, into a less toxic compound. The presence of MAK1 in this fungal pathogen is also correlated with high virulence on chick-pea. Previous genetic analysis suggested that MAK1 is located on a meiotically unstable, dispensable chromosome. The unstable nature of this chromosome facilitated MAK1 cloning by allowing us to identify a subset of genomic cosmid clones likely to contain MAK1. Truncated forms of the chromosome, generated during meiosis, were isolated from strains either able (Mak+) or unable (Mak-) to metabolize maackiain and used to probe a chromosome-specific cosmid library. Only clones that hybridized exclusively to the chromosome from the Mak+ strain were then screened for their ability to transform a Mak- isolate to the Mak+ phenotype. A 2.7 kb HindIII-PstI fragment was subcloned from a cosmid conferring MAK1 activity, and its nucleotide sequence determined. Because MAK1 transcription is not induced strongly by maackiain, a reverse transcriptase-polymerase chain reaction was required to detect MAK1 transcription in a Mak+ strain, and to isolate MAK1 cDNA fragments. Comparison of the genomic and cDNA sequences of MAK1 revealed the presence of three introns and an open reading frame encoding a protein 460 amino acids in length. Two diagnostic domains in its deduced amino acid sequence suggest MAK1 encodes a flavin-containing mono-oxygenase. MAK1 is the first gene encoding maackiain detoxification to be cloned, and is the second functional gene cloned from this dispensable chromosome. Southern analysis of genomic DNA from ascospore isolates containing MAK2, MAK3, and MAK4 indicated that MAK1 is not homologous to other known maackianin-detoxifying genes.
Brody,
Electrophoretic karyotype of Aspergillus nidulans.
1989, Pubmed
Brody,
Electrophoretic karyotype of Aspergillus nidulans.
1989,
Pubmed
Clarke,
A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.
1976,
Pubmed
Eggink,
Rubredoxin reductase of Pseudomonas oleovorans. Structural relationship to other flavoprotein oxidoreductases based on one NAD and two FAD fingerprints.
1990,
Pubmed
Farman,
Transformation frequencies are enhanced and vector DNA is targeted during retransformation of Leptosphaeria maculans, a fungal plant pathogen.
1992,
Pubmed
Frohman,
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
1988,
Pubmed
Gaffney,
Requirement of salicylic Acid for the induction of systemic acquired resistance.
1993,
Pubmed
Kinscherf,
Molecular analysis of the karyotype of Ustilago maydis.
1988,
Pubmed
Kozak,
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
1984,
Pubmed
Maloney,
A gene from the fungal plant pathogen Nectria haematococca that encodes the phytoalexin-detoxifying enzyme pisatin demethylase defines a new cytochrome P450 family.
1994,
Pubmed
,
Echinobase
Matthews,
Role of oxygenases in pisatin biosynthesis and in the fungal degradation of maackiain.
1987,
Pubmed
,
Echinobase
Miao,
A fungal gene for antibiotic resistance on a dispensable ("B") chromosome.
1991,
Pubmed
,
Echinobase
Miao,
Three Genes for Metabolism of the Phytoalexin Maackiain in the Plant Pathogen Nectria haematococca: Meiotic Instability and Relationship to a New Gene for Pisatin Demethylase.
1992,
Pubmed
,
Echinobase
Miao,
Identification and chromosomal locations of a family of cytochrome P-450 genes for pisatin detoxification in the fungus Nectria haematococca.
1991,
Pubmed
,
Echinobase
Miao,
Genetic Analysis of the Role of Phytoalexin Detoxification in Virulence of the Fungus Nectria haematococca on Chickpea (Cicer arietinum).
1992,
Pubmed
,
Echinobase
Orbach,
Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker.
1986,
Pubmed
Orbach,
A cosmid with a HyR marker for fungal library construction and screening.
1994,
Pubmed
Reimmann,
Cloning and characterization of the PDA6-1 gene encoding a fungal cytochrome P-450 which detoxifies the phytoalexin pisatin from garden pea.
1994,
Pubmed
,
Echinobase
Schwartz,
Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.
1984,
Pubmed
Schäfer,
One enzyme makes a fungal pathogen, but not a saprophyte, virulent on a new host plant.
1989,
Pubmed
,
Echinobase
Stahl,
Cutinase is not required for fungal pathogenicity on pea.
1992,
Pubmed
,
Echinobase
Straney,
Characterization of the PDA1 promoter of Nectria haematococca and identification of a region that binds a pisatin-responsive DNA binding factor.
1994,
Pubmed
,
Echinobase
Turgeon,
Development of a fungal transformation system based on selection of sequences with promoter activity.
1987,
Pubmed
VanEtten,
Location of pathogenicity genes on dispensable chromosomes in Nectria haematococca MPVI.
1994,
Pubmed
,
Echinobase
Vanetten,
Phytoalexin detoxification: importance for pathogenicity and practical implications.
1989,
Pubmed
Weltring,
Isolation of a phytoalexin-detoxification gene from the plant pathogenic fungus Nectria haematococca by detecting its expression in Aspergillus nidulans.
1988,
Pubmed
,
Echinobase
Wierenga,
Prediction of the occurrence of the ADP-binding beta alpha beta-fold in proteins, using an amino acid sequence fingerprint.
1986,
Pubmed
You,
Nucleotide sequence analysis of the Pseudomonas putida PpG7 salicylate hydroxylase gene (nahG) and its 3'-flanking region.
1991,
Pubmed
