Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Dev Biol
2010 Jan 15;3372:220-32. doi: 10.1016/j.ydbio.2009.10.030.
Show Gene links
Show Anatomy links
Nanos functions to maintain the fate of the small micromere lineage in the sea urchin embryo.
Juliano CE
,
Yajima M
,
Wessel GM
.
???displayArticle.abstract???
The translational regulator nanos is required for the survival and maintenance of primordial germ cells during embryogenesis. Three nanos homologs are present in the genome of the sea urchin Strongylocentrotus purpuratus, all of which are expressed with different timing in the small micromere lineage. This lineage is set-aside during embryogenesis and contributes to constructing the adult rudiment. Small micromeres lacking Sp-nanos1 and Sp-nanos2 undergo an extra division and are not incorporated into the coelomic pouches. Further, these cells do not accumulate Vasa protein even though they retain vasa mRNA. Larvae that develop from Sp-nanos1 and 2 knockdown embryos initially appear normal, but do not develop adult rudiments; although they are capable of eating, over time they fail to grow and eventually die. We conclude that the acquisition and maintenance of multipotency in the small micromere lineage requires nanos, which may function in part by repressing the cell cycle and regulating other multipotency factors such as vasa. This work, in combination with other recent results in Ilyanassa and Platynereis dumerilii, suggests the presence of a conserved molecular program underlying both primordial germ cell and multipotent cell specification and maintenance.
Altschul,
Basic local alignment search tool.
1990, Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Asaoka-Taguchi,
Maternal Pumilio acts together with Nanos in germline development in Drosophila embryos.
1999,
Pubmed
Cameron,
Macromere cell fates during sea urchin development.
1991,
Pubmed
,
Echinobase
Cheers,
Rapid microinjection of fertilized eggs.
2004,
Pubmed
,
Echinobase
Deshpande,
Novel functions of nanos in downregulating mitosis and transcription during the development of the Drosophila germline.
1999,
Pubmed
Draper,
nanos1 is required to maintain oocyte production in adult zebrafish.
2007,
Pubmed
Ettensohn,
Gene regulatory networks and developmental plasticity in the early sea urchin embryo: alternative deployment of the skeletogenic gene regulatory network.
2007,
Pubmed
,
Echinobase
Ettensohn,
Cell lineage conversion in the sea urchin embryo.
1988,
Pubmed
,
Echinobase
Gao,
Transfer of a large gene regulatory apparatus to a new developmental address in echinoid evolution.
2008,
Pubmed
,
Echinobase
Guo,
A Bruno-like gene is required for stem cell maintenance in planarians.
2006,
Pubmed
Hayashi,
Nanos suppresses somatic cell fate in Drosophila germ line.
2004,
Pubmed
Juliano,
Germ line determinants are not localized early in sea urchin development, but do accumulate in the small micromere lineage.
2006,
Pubmed
,
Echinobase
Kadyrova,
Translational control of maternal Cyclin B mRNA by Nanos in the Drosophila germline.
2007,
Pubmed
Kobayashi,
Essential role of the posterior morphogen nanos for germline development in Drosophila.
1996,
Pubmed
Köprunner,
A zebrafish nanos-related gene is essential for the development of primordial germ cells.
2001,
Pubmed
Lolicato,
Potential role of Nanos3 in maintaining the undifferentiated spermatogonia population.
2008,
Pubmed
Minokawa,
Expression patterns of four different regulatory genes that function during sea urchin development.
2004,
Pubmed
,
Echinobase
Mochizuki,
Expression and evolutionary conservation of nanos-related genes in Hydra.
2000,
Pubmed
Mochizuki,
Universal occurrence of the vasa-related genes among metazoans and their germline expression in Hydra.
2001,
Pubmed
Nagahara,
Transduction of full-length TAT fusion proteins into mammalian cells: TAT-p27Kip1 induces cell migration.
1998,
Pubmed
Nakahata,
Involvement of Xenopus Pumilio in the translational regulation that is specific to cyclin B1 mRNA during oocyte maturation.
2003,
Pubmed
Nakahata,
Biochemical identification of Xenopus Pumilio as a sequence-specific cyclin B1 mRNA-binding protein that physically interacts with a Nanos homolog, Xcat-2, and a cytoplasmic polyadenylation element-binding protein.
2001,
Pubmed
Palakodeti,
The PIWI proteins SMEDWI-2 and SMEDWI-3 are required for stem cell function and piRNA expression in planarians.
2008,
Pubmed
Pehrson,
The fate of the small micromeres in sea urchin development.
1986,
Pubmed
,
Echinobase
Peterson,
Set-aside cells in maximal indirect development: evolutionary and developmental significance.
1997,
Pubmed
Pfister,
Flatworm stem cells and the germ line: developmental and evolutionary implications of macvasa expression in Macrostomum lignano.
2008,
Pubmed
Rabinowitz,
Nanos is required in somatic blast cell lineages in the posterior of a mollusk embryo.
2008,
Pubmed
Raff,
Origins of the other metazoan body plans: the evolution of larval forms.
2008,
Pubmed
Ransick,
Postembryonic segregation of the germ line in sea urchins in relation to indirect development.
1996,
Pubmed
,
Echinobase
Rebscher,
Vasa unveils a common origin of germ cells and of somatic stem cells from the posterior growth zone in the polychaete Platynereis dumerilii.
2007,
Pubmed
Reddien,
SMEDWI-2 is a PIWI-like protein that regulates planarian stem cells.
2005,
Pubmed
Render,
Cell fate maps in the Ilyanassa obsoleta embryo beyond the third division.
1997,
Pubmed
Salvetti,
DjPum, a homologue of Drosophila Pumilio, is essential to planarian stem cell maintenance.
2005,
Pubmed
Sato,
Maternal Nanos represses hid/skl-dependent apoptosis to maintain the germ line in Drosophila embryos.
2007,
Pubmed
Schaner,
A conserved chromatin architecture marks and maintains the restricted germ cell lineage in worms and flies.
2003,
Pubmed
Seki,
Cellular dynamics associated with the genome-wide epigenetic reprogramming in migrating primordial germ cells in mice.
2007,
Pubmed
Seydoux,
Pathway to totipotency: lessons from germ cells.
2006,
Pubmed
Shibata,
Expression of vasa(vas)-related genes in germline cells and totipotent somatic stem cells of planarians.
1999,
Pubmed
Sly,
Who came first--larvae or adults? origins of bilaterian metazoan larvae.
2003,
Pubmed
Su,
The cell cycle program in germ cells of the Drosophila embryo.
1998,
Pubmed
Subramaniam,
nos-1 and nos-2, two genes related to Drosophila nanos, regulate primordial germ cell development and survival in Caenorhabditis elegans.
1999,
Pubmed
Suzuki,
Nanos3 maintains the germ cell lineage in the mouse by suppressing both Bax-dependent and -independent apoptotic pathways.
2008,
Pubmed
Swartz,
Localization of Vasa mRNA during early cleavage of the snail Ilyanassa.
2008,
Pubmed
Tanaka,
Study of the Lineage and Cell Cycle of Small Micromeres in Embryos of the Sea Urchin, Hemicentrotus pulcherrimus: (small micromeres/cell cycle/cell lineage/unequal cleavage/sea urchin).
1990,
Pubmed
,
Echinobase
Tsuda,
Conserved role of nanos proteins in germ cell development.
2003,
Pubmed
Voronina,
Vasa protein expression is restricted to the small micromeres of the sea urchin, but is inducible in other lineages early in development.
2008,
Pubmed
,
Echinobase
Wessel,
Direct molecular interaction of a conserved yolk granule protein in sea urchins.
2000,
Pubmed
,
Echinobase
Wong,
Major components of a sea urchin block to polyspermy are structurally and functionally conserved.
2004,
Pubmed
,
Echinobase
Yajima,
Evolutionary modification of mesenchyme cells in sand dollars in the transition from indirect to direct development.
2007,
Pubmed
,
Echinobase
