Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Regulation of intracellular pH by p90Rsk-dependent activation of an Na(+)/H(+) exchanger in starfish oocytes.
Harada K
,
Fukuda E
,
Hirohashi N
,
Chiba K
.
???displayArticle.abstract???
Starfish oocytes arrest at metaphase of the first meiotic division (MI arrest) in the ovary and resume meiosis after spawning into seawater. MI arrest is maintained by lower intracellular pH (pH(i)) and release from arrest by cellular alkalization. To elucidate pH(i) regulation in oocytes, we cloned the starfish (Asterina pectinifera) Na(+)/H(+) exchanger 3 (ApNHE3) expressed in the plasma membrane of oocytes. The cytoplasmic domain of ApNHE3 contains p90 ribosomal S6 kinase (p90Rsk) phosphorylation sites, and injection of a constitutively active p90Rsk and the upstream regulator Mos to immature oocytes, stimulated an increase in pH(i). This increase was blocked by 5-(N-ethyl-N-isopropyl)-amiloride, a NHE inhibitor, and SL0101, a specific Rsk inhibitor. The MAPK kinase (MEK) inhibitor U0126 blocked the Mos-induced, but not the p90Rsk-induced, pH(i) increase, suggesting that the Mos-MEK-MAPK-p90Rsk pathway promotes ApNHE3 activation. In a cell-free extract, the Mos-MEK-MAPK-p90Rsk pathway phosphorylates ApNHE3 at Ser-590, -606, and -673. When p90Rsk-dependent ApNHE3 phosphorylation was blocked by a dominant-negative C-terminal fragment, or neutralizing antibody, the p90Rsk-induced pH(i) increase was suppressed in immature oocytes. However, ApNHE3 is up-regulated via the upstream phosphatidylinositol 3-kinase pathway before MAPK activation and the active state is maintained until spawning, suggesting that the p90Rsk-dependent ApNHE3 phosphorylation is unlikely to be the primary regulatory mechanism involved in MI arrest exit. After meiosis is completed, unfertilized eggs maintain their elevated pH(i) ( approximately 7.4) until the onset of apoptosis. We suggest that the p90Rsk/ApNHE3-dependent elevation of pH(i) increases fertilization success by delaying apoptosis initiation.
Ammar,
Crystal structure of CHP2 complexed with NHE1-cytosolic region and an implication for pH regulation.
2006, Pubmed
Ammar,
Crystal structure of CHP2 complexed with NHE1-cytosolic region and an implication for pH regulation.
2006,
Pubmed
Brett,
Evolutionary origins of eukaryotic sodium/proton exchangers.
2005,
Pubmed
Cha,
The NHE3 juxtamembrane cytoplasmic domain directly binds ezrin: dual role in NHE3 trafficking and mobility in the brush border.
2006,
Pubmed
Chalumeau,
NHE3 activity and trafficking depend on the state of actin organization in proximal tubule.
2001,
Pubmed
Chiba,
Induction of starfish oocyte maturation by the beta gamma subunit of starfish G protein and possible existence of the subsequent effector in cytoplasm.
1993,
Pubmed
,
Echinobase
Chiba,
Induction of germinal vesicle breakdown in a cell-free preparation from starfish oocytes.
1999,
Pubmed
,
Echinobase
Deguchi,
Repetitive intracellular Ca2+ increases at fertilization and the role of Ca2+ in meiosis reinitiation from the first metaphase in oocytes of marine bivalves.
1994,
Pubmed
,
Echinobase
Deguchi,
Serotonin-induced meiosis reinitiation from the first prophase and from the first metaphase in oocytes of the marine bivalve Hiatella flaccida: respective changes in intracellular Ca2+ and pH.
1995,
Pubmed
Epel,
An analysis of the partial metabolic derepression of sea urchin eggs by ammonia: the existence of independent pathways.
1974,
Pubmed
,
Echinobase
FitzHarris,
Granulosa cells regulate oocyte intracellular pH against acidosis in preantral follicles by multiple mechanisms.
2007,
Pubmed
Frödin,
A phosphoserine-regulated docking site in the protein kinase RSK2 that recruits and activates PDK1.
2000,
Pubmed
Hanocq-Quertier,
Phosphorylation of ribosomal proteins during maturation of Xenopus laevis oocytes.
1981,
Pubmed
Harada,
Metaphase I arrest of starfish oocytes induced via the MAP kinase pathway is released by an increase of intracellular pH.
2003,
Pubmed
,
Echinobase
Kanatani,
Isolation and indentification on meiosis inducing substance in starfish Asterias amurensis.
1969,
Pubmed
,
Echinobase
Kang,
Raf-1 kinase, a potential regulator of intracellular pH in Xenopus oocytes.
1998,
Pubmed
Khaled,
Trophic factor withdrawal: p38 mitogen-activated protein kinase activates NHE1, which induces intracellular alkalinization.
2001,
Pubmed
Kishimoto,
Cell cycle arrest and release in starfish oocytes and eggs.
1998,
Pubmed
,
Echinobase
Kobayashi,
Activation of serum- and glucocorticoid-regulated protein kinase by agonists that activate phosphatidylinositide 3-kinase is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and PDK2.
1999,
Pubmed
Kozikowski,
Novel PI analogues selectively block activation of the pro-survival serine/threonine kinase Akt.
2003,
Pubmed
Kruppa,
The phosphorylation of ribosomal protein S6 from progesterone-stimulated Xenopus laevis oocytes. Kinetic studies and phosphopeptide analysis.
1983,
Pubmed
Kurashima,
The apical Na(+)/H(+) exchanger isoform NHE3 is regulated by the actin cytoskeleton.
1999,
Pubmed
Liu,
Caspase-8-mediated intracellular acidification precedes mitochondrial dysfunction in somatostatin-induced apoptosis.
2000,
Pubmed
Luo,
Physiology and pathophysiology of Na(+)/H(+) exchange isoform 1 in the central nervous system.
2007,
Pubmed
Marino,
Interaction of cell cycle kinases, microtubules, and chromatin in ascidian oocytes during meiosis.
2000,
Pubmed
Mazia,
DNA synthesis turned on in unfertilized sea urchin eggs by treatment with NH4OH.
1974,
Pubmed
,
Echinobase
Moor,
Activation of Na+/H+ exchanger-directed protein kinases in the ischemic and ischemic-reperfused rat myocardium.
2001,
Pubmed
Mori,
p90Rsk is required for G1 phase arrest in unfertilized starfish eggs.
2006,
Pubmed
,
Echinobase
Morrill,
Role of calcium in regulating intracellular pH following the stepwise release of the metabolic blocks at first-meiotic prophase and second-meiotic metaphase in amphibian oocytes.
1984,
Pubmed
Murtazina,
Na+/H+ exchanger NHE3 activity and trafficking are lipid Raft-dependent.
2006,
Pubmed
Nofer,
Low density lipoproteins inhibit the Na+/H+ antiport in human platelets via activation of p38MAP kinase.
2006,
Pubmed
Oita,
Degradation of polyubiquitinated cyclin B is blocked by the MAPK pathway at the metaphase I arrest in starfish oocytes.
2004,
Pubmed
,
Echinobase
Okumura,
Akt inhibits Myt1 in the signalling pathway that leads to meiotic G2/M-phase transition.
2002,
Pubmed
,
Echinobase
Orlowski,
Heterologous expression and functional properties of amiloride high affinity (NHE-1) and low affinity (NHE-3) isoforms of the rat Na/H exchanger.
1993,
Pubmed
Orlowski,
Delineation of transmembrane domains of the Na+/H+ exchanger that confer sensitivity to pharmacological antagonists.
1996,
Pubmed
Perdiguero,
Xp38gamma/SAPK3 promotes meiotic G(2)/M transition in Xenopus oocytes and activates Cdc25C.
2003,
Pubmed
Rangel-Mata,
Rho, Rho-kinase, and the actin cytoskeleton regulate the Na+ -H+ exchanger in sea urchin eggs.
2007,
Pubmed
,
Echinobase
Rezai,
Protooncogene product, c-mos kinase, is involved in upregulating Na+/H+ antiporter in Xenopus oocytes.
1994,
Pubmed
Sakaue,
Quantitative measurement of caspase-3 activity in a living starfish egg.
2006,
Pubmed
,
Echinobase
Sasaki,
Induction of apoptosis in starfish eggs requires spontaneous inactivation of MAPK (extracellular signal-regulated kinase) followed by activation of p38MAPK.
2004,
Pubmed
,
Echinobase
Sasaki,
Fertilization blocks apoptosis of starfish eggs by inactivation of the MAP kinase pathway.
2001,
Pubmed
,
Echinobase
Smith,
Identification of the first specific inhibitor of p90 ribosomal S6 kinase (RSK) reveals an unexpected role for RSK in cancer cell proliferation.
2005,
Pubmed
Stith,
Increased intracellular pH is not necessary for ribosomal protein s6 phosphorylation, increased protein synthesis, or germinal vesicle breakdown in Xenopus oocytes.
1985,
Pubmed
Tachibana,
c-Mos forces the mitotic cell cycle to undergo meiosis II to produce haploid gametes.
2000,
Pubmed
,
Echinobase
Takahashi,
p90(RSK) is a serum-stimulated Na+/H+ exchanger isoform-1 kinase. Regulatory phosphorylation of serine 703 of Na+/H+ exchanger isoform-1.
1999,
Pubmed
Thangaraju,
Regulation of acidification and apoptosis by SHP-1 and Bcl-2.
1999,
Pubmed
Thangaraju,
Interdependent regulation of intracellular acidification and SHP-1 in apoptosis.
1999,
Pubmed
Usui,
Involvement of mitogen-activating protein kinase and intracellular pH in the duration of the metaphase I (MI) pause of starfish oocytes after spawning.
2008,
Pubmed
,
Echinobase
Wang,
Activation of NHE3 by dexamethasone requires phosphorylation of NHE3 at Ser663 by SGK1.
2005,
Pubmed
Wiederkehr,
Acute regulation of Na/H exchanger NHE3 activity by protein kinase C: role of NHE3 phosphorylation.
1999,
Pubmed
Yamamoto,
A metaphase pause: hormone-induced maturation progresses through a long pause at the first meiotic metaphase in oocytes of the starfish, Pisaster ochraceus.
1997,
Pubmed
,
Echinobase
Yamamoto,
Involvement of Mos-MEK-MAPK pathway in cytostatic factor (CSF) arrest in eggs of the parthenogenetic insect, Athalia rosae.
2008,
Pubmed
Yu,
Functional properties of the rat Na/H exchanger NHE-2 isoform expressed in Na/H exchanger-deficient Chinese hamster ovary cells.
1993,
Pubmed
Yun,
Glucocorticoid activation of Na(+)/H(+) exchanger isoform 3 revisited. The roles of SGK1 and NHERF2.
2002,
Pubmed
Yun,
cAMP-mediated inhibition of the epithelial brush border Na+/H+ exchanger, NHE3, requires an associated regulatory protein.
1997,
Pubmed
Yun,
NHE3 kinase A regulatory protein E3KARP binds the epithelial brush border Na+/H+ exchanger NHE3 and the cytoskeletal protein ezrin.
1998,
Pubmed
Yun,
Concerted roles of SGK1 and the Na+/H+ exchanger regulatory factor 2 (NHERF2) in regulation of NHE3.
2003,
Pubmed
Zhou,
HCO3(-)/Cl(-) exchange inactivation and reactivation during mouse oocyte meiosis correlates with MEK/MAPK-regulated Ae2 plasma membrane localization.
2009,
Pubmed
