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J Microbiol Methods 2015 Jan 01;108:83-91. doi: 10.1016/j.mimet.2014.11.011.
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Characterisation of non-toxigenic Clostridium spp. strains, to use as surrogates for non-proteolytic Clostridium botulinum in chilled food challenge testing.

Parker MD , Barrett PI , Shepherd J , Price LJ , Bull SD .

Under many of the conditions studied, a two-strain cocktail of non-toxigenic Clostridium spp. was found to be suitable as a surrogate for non-proteolytic Clostridium botulinum, and has the potential for use in chilled food challenge tests measuring growth. Non-toxigenic surrogates could also be used in thermal process screening studies.

PubMed ID: 25433276
Article link: J Microbiol Methods