Dev Biol 1987 Jun 01;1212:576-9. doi: 10.1016/0012-1606(87)90193-x.

Correct cell-type-specific expression of a fusion gene injected into sea urchin eggs.

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A fusion gene construct containing the bacterial chloramphenicol acetyltransferase (CAT) gene under the control of CyIIIa actin gene regulatory sequences was injected into unfertilized eggs of the urchin Strongylocentrotus purpuratus, and early pluteus stage embryos that developed from these eggs were fixed and sectioned for analysis by in situ hybridization. A [3H]RNA antisense probe for CAT mRNA was hybridized to 5-micron embryo sections. Autoradiographic signal denoting the presence of CAT mRNA was detected only over aboral ectoderm cells, in which the CyIIIa gene is normally expressed, and not over any recognizable regions of gut or oral ectoderm included in the same sections.

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Genes referenced: CyIIIa LOC100887844 LOC100888042 LOC115917902 LOC115919910 LOC590297