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Molecules
2016 May 12;215:. doi: 10.3390/molecules21050625.
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In Vivo Anti-Cancer Mechanism of Low-Molecular-Weight Fucosylated Chondroitin Sulfate (LFCS) from Sea Cucumber Cucumaria frondosa.
Liu X
,
Liu Y
,
Hao J
,
Zhao X
,
Lang Y
,
Fan F
,
Cai C
,
Li G
,
Zhang L
,
Yu G
.
Abstract
The low-molecular-weight fucosylated chondroitin sulfate (LFCS) was prepared from native fucosylated chondroitin sulfate (FCS), which was extracted and isolated from sea cucumber Cucumaria frondosa, and the anti-cancer mechanism of LFCS on mouse Lewis lung carcinoma (LLC) was investigated. The results showed that LFCS remarkably inhibited LLC growth and metastasis in a dose-dependent manner. LFCS induced cell cycle arrest by increasing p53/p21 expression and apoptosis through activation of caspase-3 activity in LLC cells. Meanwhile, LFCS suppressed the expression of vascular endothelial growth factor (VEGF), increased the expression of tissue inhibitor of metalloproteinase-1 (TIMP-1) and downregulated the matrix metalloproteinases (MMPs) level. Furthermore, LFCS significantly suppressed the activation of ERK1/2/p38 MAPK/NF-κB pathway, which played a prime role in expression of MMPs. All of these data indicate LFCS may be used as anti-cancer drug candidates and deserve further study.
Figure 5. LFCS increased protein expression of p53, p21, and caspase-3 in LLC cells after 72 h treatment of LFCS detected by Western blot. Expression of the β-actin was used as internal control. Lane 1: vehicle-treated cells; Lane 2: 50 μg/mL LFCS-treated cells; Lane 3: 400 μg/mL LFCS-treated cells. Data were presented as mean ± SD and derived from one representative experiment performed in triplicate. * p < 0.05, ** p < 0.01, significant difference compared with control group.
Figure 6. LFCS suppressed metastasis and angiogenesis by regulating protein expression of MMP-9, TIMP-1, VEGF, p38, ERK1/2, and NF-κB in LLC cells after 72 h treatment of LFCS detected by Western blot. (A) LFCS increased TIMP-1, and decreased MMP-9 and VEGF expression; (B) LFCS suppressed NF-κB, p38 and ERK1/2 expression. Expression of the β-actin was used as internal control. Lane 1: vehicle-treated cells; Lane 2: 50 μg/mL LFCS-treated cells; Lane 3: 400 μg/mL LFCS-treated cells. Data were presented as mean ± SD and derived from one representative experiment performed in triplicate. * p < 0.05, ** p < 0.01, significant difference compared with control group.
Al Saleh,
Signalling pathways involved in endocrine resistance in breast cancer and associations with epithelial to mesenchymal transition (Review).
2011, Pubmed
Al Saleh,
Signalling pathways involved in endocrine resistance in breast cancer and associations with epithelial to mesenchymal transition (Review).
2011,
Pubmed
Babykutty,
Nimbolide retards tumor cell migration, invasion, and angiogenesis by downregulating MMP-2/9 expression via inhibiting ERK1/2 and reducing DNA-binding activity of NF-κB in colon cancer cells.
2012,
Pubmed
Borsig,
Selectin blocking activity of a fucosylated chondroitin sulfate glycosaminoglycan from sea cucumber. Effect on tumor metastasis and neutrophil recruitment.
2007,
Pubmed
,
Echinobase
Chaffer,
A perspective on cancer cell metastasis.
2011,
Pubmed
Cohen,
Caspases: the executioners of apoptosis.
1997,
Pubmed
D'Amore,
Vascular endothelial cell growth factor-a: not just for endothelial cells anymore.
2007,
Pubmed
El-Seedi,
The traditional medical uses and cytotoxic activities of sixty-one Egyptian plants: discovery of an active cardiac glycoside from Urginea maritima.
2013,
Pubmed
Ferlay,
Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012.
2015,
Pubmed
Gómez-Piña,
Role of MMPs in orchestrating inflammatory response in human monocytes via a TREM-1-PI3K-NF-κB pathway.
2012,
Pubmed
Han,
Role of p21 in apoptosis and senescence of human colon cancer cells treated with camptothecin.
2002,
Pubmed
Hanahan,
The hallmarks of cancer.
2000,
Pubmed
Hanahan,
Hallmarks of cancer: the next generation.
2011,
Pubmed
Huang,
Anti-inflammatory activities of inotilone from Phellinus linteus through the inhibition of MMP-9, NF-κB, and MAPK activation in vitro and in vivo.
2012,
Pubmed
Huang,
Prophylactic administration of fucoidan represses cancer metastasis by inhibiting vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs) in Lewis tumor-bearing mice.
2015,
Pubmed
Kitazato,
DHG, a new depolymerized holothurian glycosaminoglycan, exerts an antithrombotic effect with less bleeding than unfractionated or low molecular weight heparin, in rats.
1996,
Pubmed
Ko,
Piceatannol suppresses breast cancer cell invasion through the inhibition of MMP-9: involvement of PI3K/AKT and NF-κB pathways.
2012,
Pubmed
Larsen,
Targeted therapies for lung cancer: clinical experience and novel agents.
2011,
Pubmed
Li,
Ellagic acid induced p53/p21 expression, G1 arrest and apoptosis in human bladder cancer T24 cells.
2005,
Pubmed
Li,
Aggregation of human platelets by acidic mucopolysaccharide extracted from Stichopus japonicus Selenka.
1988,
Pubmed
,
Echinobase
Lian,
Anti-HIV-1 activity and structure-activity-relationship study of a fucosylated glycosaminoglycan from an echinoderm by targeting the conserved CD4 induced epitope.
2013,
Pubmed
López-Gómez,
Cancer in developing countries: the next most preventable pandemic. The global problem of cancer.
2013,
Pubmed
Makrilia,
The role of angiogenesis in solid tumours: an overview.
2009,
Pubmed
Meoni,
Medical treatment of advanced non-small cell lung cancer in elderly patients: a review of the role of chemotherapy and targeted agents.
2013,
Pubmed
Millau,
P53 transcriptional activities: a general overview and some thoughts.
2009,
Pubmed
Quaranta,
MMP-2, MMP-9, VEGF and CA 15.3 in breast cancer.
2007,
Pubmed
Reddy,
Role of MAP kinase in tumor progression and invasion.
2003,
Pubmed
Stamenkovic,
Matrix metalloproteinases in tumor invasion and metastasis.
2000,
Pubmed
Stennicke,
Catalytic properties of the caspases.
1999,
Pubmed
Sullivan,
ALK inhibitors in non-small cell lung cancer: the latest evidence and developments.
2016,
Pubmed
Tang,
CCL2 increases MMP-9 expression and cell motility in human chondrosarcoma cells via the Ras/Raf/MEK/ERK/NF-κB signaling pathway.
2012,
Pubmed
Travis,
The 2015 World Health Organization Classification of Lung Tumors: Impact of Genetic, Clinical and Radiologic Advances Since the 2004 Classification.
2015,
Pubmed
Valastyan,
Tumor metastasis: molecular insights and evolving paradigms.
2011,
Pubmed
Wimberger,
Explorative investigation of vascular endothelial growth factor receptor expression in primary ovarian cancer and its clinical relevance.
2014,
Pubmed
Woo,
DNA-dependent protein kinase acts upstream of p53 in response to DNA damage.
1998,
Pubmed
Wu,
Prognostic significance of MMP-9 and TIMP-1 serum and tissue expression in breast cancer.
2008,
Pubmed
Wu,
Anticoagulant and antithrombotic evaluation of native fucosylated chondroitin sulfates and their derivatives as selective inhibitors of intrinsic factor Xase.
2015,
Pubmed
Xiong,
p21 is a universal inhibitor of cyclin kinases.
1993,
Pubmed
Xue,
Anticancer properties and mechanisms of fucoidan on mouse breast cancer in vitro and in vivo.
2012,
Pubmed
Yang,
Novel branch patterns and anticoagulant activity of glycosaminoglycan from sea cucumber Apostichopus japonicus.
2015,
Pubmed
,
Echinobase
Zhao,
Holothurian glycosaminoglycan inhibits metastasis and thrombosis via targeting of nuclear factor-κB/tissue factor/Factor Xa pathway in melanoma B16F10 cells.
2013,
Pubmed
Zubair,
Role of nuclear factor-ĸB in breast and colorectal cancer.
2013,
Pubmed