Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mar Biotechnol (NY)
2011 Jun 01;133:536-43. doi: 10.1007/s10126-010-9324-9.
Show Gene links
Show Anatomy links
Ca2+, Mg2+-dependent DNase involvement in apoptotic effects in spermatozoa of sea urchin Strongylocentrotus intermedius induced by two-headed sphingolipid rhizochalin.
Sibirtsev JT
,
Shastina VV
,
Menzorova NI
,
Makarieva TN
,
Rasskazov VA
.
Abstract
Previously, we have purified three distinct DNases from spermatozoa of sea urchin Strongylocentrotus intermedius and we suppose the role of Ca(2+), Mg(2+)-dependent DNase (Ca, Mg-DNase) in apoptosis of spermatozoa. Two-headed sphingolipid rhizochalin (Rhz) induced characteristic apoptotic nuclear chromatin changes, internucleosomal DNA cleavage, and activation of caspase-9, caspase-8, and caspase-3 in spermatozoa as was shown by fluorescence Hoechst 33342/PI/FDA analysis, DNA fragmentation assay, and fluorescence caspase inhibitors FAM-LEHD-fmk, FAM-IETD-fmk, and FAM-DEVD-fmk, respectively. Inhibitor of caspase-3 z-DEVD-fmk subdued Rhz-induced internucleosomal ladder formation, which confirmed the major role of caspase-3 in apoptotic DNA cleavage probably through Ca, Mg-DNase activation. Participation of sea urchin Ca, Mg-DNase in apoptosis of spermatozoa was demonstrated by ions Zn(2+) blocking of Rhz-induced DNA fragmentation due to direct inhibition of the Ca, Mg-DNase and internucleosomal cleavage of HeLa S and Vero E6 cell nuclei chromatin by highly purified Ca, Mg-DNase.
Allan,
The structure of histone H1 and its location in chromatin.
1980, Pubmed
Allan,
The structure of histone H1 and its location in chromatin.
1980,
Pubmed
Anzar,
Sperm apoptosis in fresh and cryopreserved bull semen detected by flow cytometry and its relationship with fertility.
2002,
Pubmed
Aziz,
The relationship between human sperm apoptosis, morphology and the sperm deformity index.
2007,
Pubmed
Boaz,
Mouse spermatozoa contain a nuclease that is activated by pretreatment with EGTA and subsequent calcium incubation.
2008,
Pubmed
Jin,
Differential induction of apoptosis of leukemic cells by rhizochalin, two headed sphingolipids from sponge and its derivatives.
2009,
Pubmed
Marchetti,
Staining of human sperm with fluorochrome-labeled inhibitor of caspases to detect activated caspases: correlation with apoptosis and sperm parameters.
2004,
Pubmed
Martin,
Kinetics of occurrence of some features of apoptosis during the cryopreservation process of bovine spermatozoa.
2007,
Pubmed
Oehninger,
Presence and significance of somatic cell apoptosis markers in human ejaculated spermatozoa.
2003,
Pubmed
Ortega-Ferrusola,
Detection of "apoptosis-like" changes during the cryopreservation process in equine sperm.
2008,
Pubmed
Paasch,
Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa.
2004,
Pubmed
Pandey,
Identification of a novel 97 kDa endonuclease capable of internucleosomal DNA cleavage.
1997,
Pubmed
Peitsch,
The apoptosis endonucleases: cleaning up after cell death?
1994,
Pubmed
Print,
Germ cell suicide: new insights into apoptosis during spermatogenesis.
2000,
Pubmed
Shaman,
Topoisomerase IIB and an extracellular nuclease interact to digest sperm DNA in an apoptotic-like manner.
2006,
Pubmed
Shastina,
Purification and characteristics of Ca2+,Mg2+- and Ca2+,Mn2+-dependent and acid DNases from spermatozoa of the sea urchin Strongylocentrotus intermedius.
2003,
Pubmed
,
Echinobase
Shi,
Mechanisms of caspase activation and inhibition during apoptosis.
2002,
Pubmed
Shiokawa,
Purification and properties of DNase gamma from apoptotic rat thymocytes.
1997,
Pubmed
Sotolongo,
An endogenous nuclease in hamster, mouse, and human spermatozoa cleaves DNA into loop-sized fragments.
2005,
Pubmed
Voronina,
Apoptosis in sea urchin oocytes, eggs, and early embryos.
2001,
Pubmed
,
Echinobase
Widlak,
Discovery, regulation, and action of the major apoptotic nucleases DFF40/CAD and endonuclease G.
2005,
Pubmed
Wündrich,
Activation of caspases in human spermatozoa during cryopreservation--an immunoblot study.
2006,
Pubmed
Yakovlev,
Role of DNAS1L3 in Ca2+- and Mg2+-dependent cleavage of DNA into oligonucleosomal and high molecular mass fragments.
1999,
Pubmed
Yoshihara,
Evidence for adenosine diphosphate ribosylation of Ca2+, Mg2+-dependent endonuclease.
1975,
Pubmed
