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J Biol Chem
2011 Mar 25;28612:10305-15. doi: 10.1074/jbc.M110.200576.
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Galactose recognition by a tetrameric C-type lectin, CEL-IV, containing the EPN carbohydrate recognition motif.
Hatakeyama T
,
Kamiya T
,
Kusunoki M
,
Nakamura-Tsuruta S
,
Hirabayashi J
,
Goda S
,
Unno H
.
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CEL-IV is a C-type lectin isolated from a sea cucumber, Cucumaria echinata. This lectin is composed of four identical C-type carbohydrate-recognition domains (CRDs). X-ray crystallographic analysis of CEL-IV revealed that its tetrameric structure was stabilized by multiple interchain disulfide bonds among the subunits. Although CEL-IV has the EPN motif in its carbohydrate-binding sites, which is known to be characteristic of mannose binding C-type CRDs, it showed preferential binding of galactose and N-acetylgalactosamine. Structural analyses of CEL-IV-melibiose and CEL-IV-raffinose complexes revealed that their galactose residues were recognized in an inverted orientation compared with mannose binding C-type CRDs containing the EPN motif, by the aid of a stacking interaction with the side chain of Trp-79. Changes in the environment of Trp-79 induced by binding to galactose were detected by changes in the intrinsic fluorescence and UV absorption spectra of WT CEL-IV and its site-directed mutants. The binding specificity of CEL-IV toward complex oligosaccharides was analyzed by frontal affinity chromatography using various pyridylamino sugars, and the results indicate preferential binding to oligosaccharides containing GalĪ²1-3/4(FucĪ±1-3/4)GlcNAc structures. These findings suggest that the specificity for oligosaccharides may be largely affected by interactions with amino acid residues in the binding site other than those determining the monosaccharide specificity.
Collaborative Computational Project, Number 4,
The CCP4 suite: programs for protein crystallography.
1994, Pubmed
Collaborative Computational Project, Number 4,
The CCP4 suite: programs for protein crystallography.
1994,
Pubmed
Cowgill,
Fluorescence and protein structure. X. Reappraisal of solvent and structural effects.
1967,
Pubmed
Drickamer,
Engineering galactose-binding activity into a C-type mannose-binding protein.
1992,
Pubmed
Drickamer,
C-type lectin-like domains.
1999,
Pubmed
Drickamer,
Mannose-binding proteins isolated from rat liver contain carbohydrate-recognition domains linked to collagenous tails. Complete primary structures and homology with pulmonary surfactant apoprotein.
1986,
Pubmed
Emsley,
Coot: model-building tools for molecular graphics.
2004,
Pubmed
Endo,
Site of action of a Vero toxin (VT2) from Escherichia coli O157:H7 and of Shiga toxin on eukaryotic ribosomes. RNA N-glycosidase activity of the toxins.
1988,
Pubmed
Gourdine,
High affinity interaction between a bivalve C-type lectin and a biantennary complex-type N-glycan revealed by crystallography and microcalorimetry.
2008,
Pubmed
Hatakeyama,
Amino acid sequence and carbohydrate-binding analysis of the N-acetyl-D-galactosamine-specific C-type lectin, CEL-I, from the Holothuroidea, Cucumaria echinata.
2002,
Pubmed
,
Echinobase
Hatakeyama,
Characterization of a recombinant C-type lectin, rCEL-IV, expressed in Escherichia coli cells using a synthetic gene.
2006,
Pubmed
,
Echinobase
Hatakeyama,
C-type lectin-like carbohydrate recognition of the hemolytic lectin CEL-III containing ricin-type -trefoil folds.
2007,
Pubmed
,
Echinobase
Hatakeyama,
Purification and characterization of four Ca(2+)-dependent lectins from the marine invertebrate, Cucumaria echinata.
1994,
Pubmed
,
Echinobase
Hatakeyama,
Interaction of the hemolytic lectin CEL-III from the marine invertebrate Cucumaria echinata with the erythrocyte membrane.
1995,
Pubmed
,
Echinobase
Hatakeyama,
Amino acid sequence of a C-type lectin CEL-IV from the marine invertebrate Cucumaria echinata.
1995,
Pubmed
,
Echinobase
Hazes,
The (QxW)3 domain: a flexible lectin scaffold.
1996,
Pubmed
Hirabayashi,
Lectin-based structural glycomics: glycoproteomics and glycan profiling.
2004,
Pubmed
Ho,
Human RegIV protein adopts a typical C-type lectin fold but binds mannan with two calcium-independent sites.
2010,
Pubmed
Hosono,
Purification, characterization, cDNA cloning, and expression of asialofetuin-binding C-type lectin from eggs of shishamo smelt (Osmerus [Spirinchus] lanceolatus).
2005,
Pubmed
Iobst,
Binding of sugar ligands to Ca(2+)-dependent animal lectins. II. Generation of high-affinity galactose binding by site-directed mutagenesis.
1994,
Pubmed
Kilpatrick,
Animal lectins: a historical introduction and overview.
2002,
Pubmed
Kuramoto,
Cytotoxicity of a GalNAc-specific C-type lectin CEL-I toward various cell lines.
2005,
Pubmed
,
Echinobase
Kuroda,
Targeting activating transcription factor 3 by Galectin-9 induces apoptosis and overcomes various types of treatment resistance in chronic myelogenous leukemia.
2010,
Pubmed
Lehotzky,
Molecular basis for peptidoglycan recognition by a bactericidal lectin.
2010,
Pubmed
Liu,
Apoptosis-inducing effect and structural basis of Polygonatum cyrtonema lectin and chemical modification properties on its mannose-binding sites.
2008,
Pubmed
Liu,
Molecular cloning, characterization and expression analysis of a putative C-type lectin (Fclectin) gene in Chinese shrimp Fenneropenaeus chinensis.
2007,
Pubmed
McCoy,
Likelihood-enhanced fast translation functions.
2005,
Pubmed
Murshudov,
Refinement of macromolecular structures by the maximum-likelihood method.
1997,
Pubmed
Nakamura,
Comparative analysis of carbohydrate-binding properties of two tandem repeat-type Jacalin-related lectins, Castanea crenata agglutinin and Cycas revoluta leaf lectin.
2005,
Pubmed
Ng,
Orientation of bound ligands in mannose-binding proteins. Implications for multivalent ligand recognition.
2002,
Pubmed
Olsnes,
The history of ricin, abrin and related toxins.
2004,
Pubmed
Otwinowski,
Processing of X-ray diffraction data collected in oscillation mode.
1997,
Pubmed
Poget,
The structure of a tunicate C-type lectin from Polyandrocarpa misakiensis complexed with D -galactose.
1999,
Pubmed
Sugawara,
Characteristic recognition of N-acetylgalactosamine by an invertebrate C-type Lectin, CEL-I, revealed by X-ray crystallographic analysis.
2004,
Pubmed
,
Echinobase
SundarRaj,
Glycodelin A triggers T cell apoptosis through a novel calcium-independent galactose-binding lectin activity.
2009,
Pubmed
Suzuki,
A calcium-dependent galactose-binding lectin from the tunicate Polyandrocarpa misakiensis. Isolation, characterization, and amino acid sequence.
1990,
Pubmed
,
Echinobase
Tasumi,
Primary structure and characteristics of a lectin from skin mucus of the Japanese eel Anguilla japonica.
2002,
Pubmed
Uchida,
Crystal structure of the hemolytic lectin CEL-III isolated from the marine invertebrate Cucumaria echinata: implications of domain structure for its membrane pore-formation mechanism.
2004,
Pubmed
,
Echinobase
YANARI,
Interpretation of the ultraviolet spectral changes of proteins.
1960,
Pubmed
Yamanishi,
CEL-I, an invertebrate N-acetylgalactosamine-specific C-type lectin, induces TNF-alpha and G-CSF production by mouse macrophage cell line RAW264.7 cells.
2007,
Pubmed
,
Echinobase
Yamasaki,
Ricin D-saccharide interaction as studied by ultraviolet difference spectroscopy.
1985,
Pubmed
Zelensky,
The C-type lectin-like domain superfamily.
2005,
Pubmed
