Int J Biol Macromol 2019 Jul 15;133:44-50. doi: 10.1016/j.ijbiomac.2019.04.080.

Comparison of chain conformation properties of bio-active fucosylated chondroitin sulfates from two different sea cucumbers.

Abstract
The present studies aimed to investigate the chain conformation of fucosylated chondroitin sulfates from two different sea cucumbers Apostichopus japonicus (fCS-Aj), and Holothuria mexicana (fCS-Hm). The chain conformations were determined by SEC-MALLS-Visc-RI, indicating that the chain conformations of fCS-Aj and fCS-Hm were random coil and extended linear chain structure in PBS, respectively. Our results suggested that the formation of linear chain of fCS were due to a synergistic effect of molecular weight, sulfate content and sulfation patterns. The worm-like cylinder model indicated that fCS-Aj possessed more flexible chains compared with fCS-Hm. fCS-Aj and fCS-Hm all exhibited as negatively charged polymers with good thermal stability. The present studies were important for understanding the structure-activities relationship of fCSs.

PubMed ID: 30986461
Article link: Int J Biol Macromol


Genes referenced: LOC100887844