Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
???displayArticle.abstract???
Messenger RNA was prepared from developing sea urchin gastrulae by puromycin release from polyribosomes. Approximately 60% of the total mRNA radioactivity of the postnuclear supernatant was recovered and shown to be free of any other labeled RNA species such as ribosomal and nuclear RNA. The mRNA was examined by hybridization to DNA present in great excess. The mRNA hybridizes almost exclusively with nonrepetitive DNA. Almost all of the messenger RNA molecules of sea urchin gastrulae therefore consist of transcripts from nonrepetitive sequences. It appears that the structural genes expressed at this stage are typically not repeated in the genome and the mRNA does not include recognizable repetitive sequence.
Blobel,
Release, identification, and isolation of messenger RNA from mammalian ribosomes.
1971, Pubmed
Blobel,
Release, identification, and isolation of messenger RNA from mammalian ribosomes.
1971,
Pubmed
Brandhorst,
Stabilities of nuclear and messenger RNA molecules in sea urchin embryos.
1972,
Pubmed
,
Echinobase
Britten,
Gene regulation for higher cells: a theory.
1969,
Pubmed
Davidson,
General interspersion of repetitive with non-repetitive sequence elements in the DNA of Xenopus.
1973,
Pubmed
Dina,
Hybridization properties and sequence arrangement in a population of mRNAs.
1973,
Pubmed
Firtel,
Isolation and hybridization kinetics of messenger RNA from Dictyostelium discoideum.
1972,
Pubmed
Gelderman,
Transcription of nonrepeated DNA in neonatal and fetal mice.
1971,
Pubmed
Greenberg,
Hybridization properties of DNA sequences directing the synthesis of messenger RNA and heterogeneous nuclear RNA.
1971,
Pubmed
HINEGARDNER,
The isolation of nuclei from eggs and embryos of the sea urchin.
1962,
Pubmed
,
Echinobase
Humphreys,
Measurements of messenger RNA entering polysomes upon fertilization of sea urchin eggs.
1971,
Pubmed
,
Echinobase
Hutton,
Renaturation of bacteriophage phiX174 DNA-RNA hybrid: RNA length effect and nucleation rate constant.
1973,
Pubmed
Kedes,
Synthesis of nuclear and chromosomal proteins on light polyribosomes during cleavage in the sea urchin embryo.
1969,
Pubmed
,
Echinobase
Kedes,
Reiteration and clustering of DNA sequences complementary to histone messenger RNA.
1971,
Pubmed
Melli,
DNA-RNA hybridization in vast DNA excess.
1971,
Pubmed
Noll,
Characterization of macromolecules by constant velocity sedimentation.
1967,
Pubmed
Weinberg,
Genes coding for polysomal 9S RNA of sea urchins: conservation and divergence.
1972,
Pubmed
,
Echinobase
Wilt,
Isolation of chromatin bearing nascent RNA from nuclei of sea urchin embryos.
1971,
Pubmed
,
Echinobase