Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Biochem J
2001 Dec 01;360Pt 2:413-9. doi: 10.1042/0264-6021:3600413.
Show Gene links
Show Anatomy links
A constitutive 70 kDa heat-shock protein is localized on the fibres of spindles and asters at metaphase in an ATP-dependent manner: a new chaperone role is proposed.
Agueli C
,
Geraci F
,
Giudice G
,
Chimenti L
,
Cascino D
,
Sconzo G
.
???displayArticle.abstract???
In the present study, double immunofluorescence and immunoblot analysis have been used to show that centrosomes, isolated from Paracentrotus lividus sea urchin embryos at the first mitotic metaphase, contain the constitutive chaperone, heat-shock protein (HSP) 70. More specifically, we demonstrate that centrosomes contain only the HSP70-d isoform, which is one of the four isoforms identified in P. lividus. We also provide evidence that p34(cell division control kinase-2) and t complex polypeptide-1 (TCP-1) alpha, a subunit of the TCP-1 complex, are localized on the centrosomes. Furthermore, inhibition of TCP-1 in vivo, via microinjecting an anti-(TCP-1 alpha) antibody into P. lividus eggs before fertilization, either impaired mitosis or induced severe malformations in more than 50% of embryos. In addition, we have isolated the whole mitotic apparatus and shown that HSP70 localizes on the fibres of spindles and asters, and binds them in an ATP-dependent manner. These observations suggest that HSP70 has a chaperone role in assisting the TCP-1 complex in tubulin folding, when localized on centrosomes, and during the assembling and disassembling of the mitotic apparatus, when localized on the fibres of spindles and asters.
Agashe,
Roles of molecular chaperones in cytoplasmic protein folding.
2000, Pubmed
Agashe,
Roles of molecular chaperones in cytoplasmic protein folding.
2000,
Pubmed
Barouch,
ATPase activity associated with the uncoating of clathrin baskets by Hsp70.
1994,
Pubmed
Barthelmes,
Active DNA topoisomerase IIalpha is a component of the salt-stable centrosome core.
2000,
Pubmed
Brown,
Molecular chaperones and the centrosome. A role for HSP 73 in centrosomal repair following heat shock treatment.
1996,
Pubmed
Brown,
Molecular chaperones and the centrosome. A role for TCP-1 in microtubule nucleation.
1996,
Pubmed
Chirico,
70K heat shock related proteins stimulate protein translocation into microsomes.
1988,
Pubmed
Deshaies,
A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides.
1988,
Pubmed
Dix,
HSP70-2 is required for desynapsis of synaptonemal complexes during meiotic prophase in juvenile and adult mouse spermatocytes.
1997,
Pubmed
Ellis,
Molecular chaperones.
1991,
Pubmed
Frydman,
Function in protein folding of TRiC, a cytosolic ring complex containing TCP-1 and structurally related subunits.
1992,
Pubmed
Greene,
Effect of nucleotide on the binding of peptides to 70-kDa heat shock protein.
1995,
Pubmed
Joshi,
Microtubule organizing centers and gamma-tubulin.
1994,
Pubmed
Khodjakov,
The sudden recruitment of gamma-tubulin to the centrosome at the onset of mitosis and its dynamic exchange throughout the cell cycle, do not require microtubules.
1999,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Liang,
Molecular chaperones and the cytoskeleton.
1997,
Pubmed
Lindquist,
The heat-shock proteins.
1988,
Pubmed
Melki,
Cytoplasmic chaperonin containing TCP-1: structural and functional characterization.
1997,
Pubmed
Mitchison,
Microtubule assembly nucleated by isolated centrosomes.
1984,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed
Ou,
A subset of centrosomal proteins are arranged in a tubular conformation that is reproduced during centrosome duplication.
2000,
Pubmed
Parsell,
The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins.
1993,
Pubmed
Perret,
Identification of an HSP70-related protein associated with the centrosome from dinoflagellates to human cells.
1995,
Pubmed
Rattner,
hsp70 is localized to the centrosome of dividing HeLa cells.
1991,
Pubmed
Riabowol,
Heat shock is lethal to fibroblasts microinjected with antibodies against hsp70.
1988,
Pubmed
Sconzo,
Identification and characterization of a constitutive HSP75 in sea urchin embryos.
1997,
Pubmed
,
Echinobase
Sconzo,
Constitutive hsp70 is essential to mitosis during early cleavage of Paracentrotus lividus embryos: the blockage of constitutive hsp70 impairs mitosis.
1999,
Pubmed
,
Echinobase
Silver,
Isolation of mitotic apparatus containing vesicles with calcium sequestration activity.
1980,
Pubmed
,
Echinobase
Thompson-Coffe,
Cold-treated centrosome: isolation of centrosomes from mitotic sea urchin eggs, production of an anticentrosomal antibody, and novel ultrastructural imaging.
1996,
Pubmed
,
Echinobase
Wigley,
Dynamic association of proteasomal machinery with the centrosome.
1999,
Pubmed
Zhu,
HSP70-2 is required for CDC2 kinase activity in meiosis I of mouse spermatocytes.
1997,
Pubmed