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The effect of Euryale ferox (Makhana), an herb of aquatic origin, on myocardial ischemic reperfusion injury.
Das S
,
Der P
,
Raychaudhuri U
,
Maulik N
,
Das DK
.
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Fox nut or gorgon nut (Euryale ferox--Family Nymphaeaceae), popularly known as Makhana, has been widely used in traditional oriental medicine to cure a variety of diseases including kidney problems, chronic diarrhea, excessive leucorrhea and hypofunction of the spleen. Based on the recent studies revealing antioxidant activities of Euryale ferox and its glucosides composition, we sought to determine if Euryale ferox seeds (Makhana) could reduce myocardial ischemic reperfusion injury. Two different models were used: acute model, where isolated rat hearts were preperfused for 15 min with Krebs Henseleit bicarbonate (KHB) buffer containing three different doses of makhana (25, 125 or 250 microg/ml) followed by 30 min of ischemia and 2 h of reperfusion; and chronic model, where rats were given two different doses of makhana (250 and 500 mg/kg/day) for 21 days, after which isolated hearts were subjected to 30 min of ischemia followed by 2 h of reperfusion. In both cases, the hearts of the Makhana treated rats were resistant to ischemic reperfusion injury as evidenced by their improved post-ischemic ventricular function and reduced myocardial infarct size. Antibody array technique was used to identify the cardioprotective proteins. The Makhana-treated hearts had increased amounts of thioredoxin-1 (Trx-1) and thioredoxin-related protein-32 (TRP32) compared to the control hearts. Western blot analysis confirmed increased expression of TRP32 and thioredoxin proteins. In vitro studies revealed that Makhana extracts had potent reactive oxygen species scavenging activities. Taken together, the results of this study demonstrate cardioprotective properties of Makhana and suggest that such cardioprotective properties may be linked with the ability of makhana to induce TRP32 and Trx-1 proteins and to scavenge ROS.
Arnér,
Physiological functions of thioredoxin and thioredoxin reductase.
2000, Pubmed
Arnér,
Physiological functions of thioredoxin and thioredoxin reductase.
2000,
Pubmed
Das,
High-performance liquid chromatographic detection of hydroxylated benzoic acids as an indirect measure of hydroxyl radical in heart: its possible link with the myocardial reperfusion injury.
1991,
Pubmed
Das,
Pathophysiology of superoxide radical as potential mediator of reperfusion injury in pig heart.
1986,
Pubmed
Das,
Antioxidant effectiveness in ischemia-reperfusion tissue injury.
1994,
Pubmed
Engelman,
Hypoxic preconditioning preserves antioxidant reserve in the working rat heart.
1995,
Pubmed
Hattori,
Pharmacological preconditioning with resveratrol: role of nitric oxide.
2002,
Pubmed
Lee,
Purification, molecular cloning, and characterization of TRP32, a novel thioredoxin-related mammalian protein of 32 kDa.
1998,
Pubmed
Lee,
Antioxidant activity of extracts from Euryale ferox seed.
2002,
Pubmed
,
Echinobase
Mathur,
Antibody-array technique reveals overexpression of important DNA-repair proteins during cardiac ischemic preconditioning.
2005,
Pubmed
Mathur,
Potential candidates for ischemic preconditioning-associated vascular growth pathways revealed by antibody array.
2005,
Pubmed
Matthews,
Thioredoxin regulates the DNA binding activity of NF-kappa B by reduction of a disulphide bond involving cysteine 62.
1992,
Pubmed
Maulik,
Evaluation of antioxidant effectiveness of a few herbal plants.
1997,
Pubmed
Maulik,
An essential role of NFkappaB in tyrosine kinase signaling of p38 MAP kinase regulation of myocardial adaptation to ischemia.
1998,
Pubmed
Myrset,
DNA and redox state induced conformational changes in the DNA-binding domain of the Myb oncoprotein.
1993,
Pubmed
Nakamura,
Measurements of plasma glutaredoxin and thioredoxin in healthy volunteers and during open-heart surgery.
1998,
Pubmed
Normén,
Combination of phytosterols and omega-3 fatty acids: a potential strategy to promote cardiovascular health.
2004,
Pubmed
Ray,
The red wine antioxidant resveratrol protects isolated rat hearts from ischemia reperfusion injury.
1999,
Pubmed
Sato,
SAPKs regulation of ischemic preconditioning.
2000,
Pubmed
Turoczi,
Thioredoxin redox signaling in the ischemic heart: an insight with transgenic mice overexpressing Trx1.
2003,
Pubmed
Zhao,
Glucosylsterols in extracts of Euryale ferox identified by high resolution NMR and mass spectrometry.
1989,
Pubmed
,
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