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Mol Cell Biol
1995 Mar 01;153:1254-64. doi: 10.1128/MCB.15.3.1254.
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The embryonic enhancer-binding protein SSAP contains a novel DNA-binding domain which has homology to several RNA-binding proteins.
DeAngelo DJ
,
DeFalco J
,
Rybacki L
,
Childs G
.
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Stage-specific activator protein (SSAP) is a 43-kDa polypeptide that binds to an enhancer element of the sea urchin late histone H1 gene. This enhancer element mediates the transcriptional activation of the late histone H1 gene in a temporally specific manner at the mid-blastula stage of embryogenesis. We have cloned cDNAs encoding SSAP by using polyclonal antibodies raised against purified SSAP to screen expression libraries. SSAP is unrelated to previously characterized transcription factors; however, it exhibits striking homology to a large family of proteins involved in RNA processing. The protein is a sequence-specific DNA-binding protein that recognizes both single- and double-stranded DNA. The DNA-binding domain of the protein was localized to the conserved RNA recognition motif (RRM). In addition to tandem copies of this conserved domain, SSAP contains a central domain that is rich in glutamine and glycine and a C-terminal domain that is enriched in serine, threonine, and basic amino acids. Overexpression of SSAP in sea urchin embryos by microinjection of either synthetic mRNA or an SSAP expression vector results in four- to eightfold transactivation of target reporter genes that contain the enhancer sequence. Transactivation occurs beginning only at the mid-blastula stage of development, suggesting that SSAP must be modified in a stage-specific manner in order to activate transcription. In addition, there are a number of other RRM-containing proteins that contain glutamine-rich regions which are postulated to function in the regulation of RNA processing. Instead, we suggest that SSAP is a member of a family of glutamine-rich RRM proteins which constitute a novel class of transcription factors.
Bellen,
The Drosophila couch potato protein is expressed in nuclei of peripheral neuronal precursors and shows homology to RNA-binding proteins.
1992, Pubmed
Bellen,
The Drosophila couch potato protein is expressed in nuclei of peripheral neuronal precursors and shows homology to RNA-binding proteins.
1992,
Pubmed
Boggs,
Regulation of sexual differentiation in D. melanogaster via alternative splicing of RNA from the transformer gene.
1987,
Pubmed
Cameron,
Ligand-dependent stimulation of introduced mammalian brain receptors alters spicule symmetry and other morphogenetic events in sea urchin embryos.
1994,
Pubmed
,
Echinobase
Cantor,
DNA choreography.
1981,
Pubmed
Childs,
Sea urchin (lytechinus pictus) late-stage histone H3 and H4 genes: characterization and mapping of a clustered but nontandemly linked multigene family.
1982,
Pubmed
,
Echinobase
Cohen,
Stage-specific switches in histone synthesis during embryogenesis of the sea urchin.
1975,
Pubmed
,
Echinobase
Colin,
Rapid repetitive microinjection.
1986,
Pubmed
,
Echinobase
Courey,
Synergistic activation by the glutamine-rich domains of human transcription factor Sp1.
1989,
Pubmed
Courey,
Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif.
1988,
Pubmed
Crozat,
Fusion of CHOP to a novel RNA-binding protein in human myxoid liposarcoma.
1993,
Pubmed
DeAngelo,
Purification and characterization of the stage-specific embryonic enhancer-binding protein SSAP-1.
1993,
Pubmed
,
Echinobase
Delattre,
Gene fusion with an ETS DNA-binding domain caused by chromosome translocation in human tumours.
1992,
Pubmed
DiLiberto,
Developmental control of promoter-specific factors responsible for the embryonic activation and inactivation of the sea urchin early histone H3 gene.
1989,
Pubmed
,
Echinobase
Dreyfuss,
hnRNP proteins and the biogenesis of mRNA.
1993,
Pubmed
Duncan,
A sequence-specific, single-strand binding protein activates the far upstream element of c-myc and defines a new DNA-binding motif.
1994,
Pubmed
Gaillard,
Sequence-specific single-strand-binding protein for the simian virus 40 early promoter stimulates transcription in vitro.
1990,
Pubmed
Gan,
Regulatory elements from the related spec genes of Strongylocentrotus purpuratus yield different spatial patterns with a lacZ reporter gene.
1990,
Pubmed
,
Echinobase
Grange,
Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif.
1987,
Pubmed
Hentschel,
Homocopolymer sequences in the spacer of a sea urchin histone gene repeat are sensitive to S1 nuclease.
1982,
Pubmed
,
Echinobase
Hoey,
Divergent homeo box proteins recognize similar DNA sequences in Drosophila.
1988,
Pubmed
Ito,
Synthesis and turnover of late H2B histone mRNA in developing embryos of the sea urchin, Strongylocentrotus purpuratus.
1988,
Pubmed
,
Echinobase
Jantzen,
Nucleolar transcription factor hUBF contains a DNA-binding motif with homology to HMG proteins.
1990,
Pubmed
Kenan,
RNA recognition: towards identifying determinants of specificity.
1991,
Pubmed
Knowles,
Comparison of the late H1 histone genes of the sea urchins Lytechinus pictus and Strongelocentrotus purpuratus.
1986,
Pubmed
,
Echinobase
Knowles,
Temporal expression of late histone messenger RNA in the sea urchin Lytechinus pictus.
1984,
Pubmed
,
Echinobase
Knowles,
Isolation, characterization, and expression of the gene encoding the late histone subtype H1-gamma of the sea urchin Strongylocentrotus purpuratus.
1987,
Pubmed
,
Echinobase
Krainer,
Functional expression of cloned human splicing factor SF2: homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators.
1991,
Pubmed
Lai,
Characterization of the structure and transcriptional patterns of the gene encoding the late histone subtype H1-beta of the sea urchin Strongylocentrotus purpuratus.
1988,
Pubmed
,
Echinobase
Lai,
Both basal and ontogenic promoter elements affect the timing and level of expression of a sea urchin H1 gene during early embryogenesis.
1988,
Pubmed
,
Echinobase
Lai,
An embryonic enhancer determines the temporal activation of a sea urchin late H1 gene.
1989,
Pubmed
,
Echinobase
Larsen,
An altered DNA conformation detected by S1 nuclease occurs at specific regions in active chick globin chromatin.
1982,
Pubmed
La Teana,
Identification of a cold shock transcriptional enhancer of the Escherichia coli gene encoding nucleoid protein H-NS.
1991,
Pubmed
Mancebo,
Structure and expression of the Drosophila melanogaster gene for the U1 small nuclear ribonucleoprotein particle 70K protein.
1990,
Pubmed
Maxson,
Expression and organization of histone genes.
1983,
Pubmed
,
Echinobase
May,
The Ewing's sarcoma EWS/FLI-1 fusion gene encodes a more potent transcriptional activator and is a more powerful transforming gene than FLI-1.
1993,
Pubmed
McMahon,
Inducible expression of a cloned heat shock fusion gene in sea urchin embryos.
1984,
Pubmed
,
Echinobase
Merrill,
Phenylalanines that are conserved among several RNA-binding proteins form part of a nucleic acid-binding pocket in the A1 heterogeneous nuclear ribonucleoprotein.
1988,
Pubmed
Nickol,
DNA conformation at the 5' end of the chicken adult beta-globin gene.
1983,
Pubmed
Nordheim,
Negatively supercoiled simian virus 40 DNA contains Z-DNA segments within transcriptional enhancer sequences.
1983,
Pubmed
Olson,
Interaction of nucleolar phosphoprotein C23 with cloned segments of rat ribosomal deoxyribonucleic acid.
1983,
Pubmed
Pabo,
Transcription factors: structural families and principles of DNA recognition.
1992,
Pubmed
Query,
A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein.
1989,
Pubmed
Ranjan,
Masking mRNA from translation in somatic cells.
1993,
Pubmed
Santoro,
Identification of single-stranded-DNA-binding proteins that interact with muscle gene elements.
1991,
Pubmed
Sarkar,
Access to a messenger RNA sequence or its protein product is not limited by tissue or species specificity.
1989,
Pubmed
Scherly,
Major determinants of the specificity of interaction between small nuclear ribonucleoproteins U1A and U2B'' and their cognate RNAs.
1990,
Pubmed
Schindelin,
Universal nucleic acid-binding domain revealed by crystal structure of the B. subtilis major cold-shock protein.
1993,
Pubmed
Schon,
Conformation of promoter DNA: fine mapping of S1-hypersensitive sites.
1983,
Pubmed
Simpson,
Modulation of nucleosome structure by histone subtypes in sea urchin embryos.
1981,
Pubmed
,
Echinobase
Studier,
Use of T7 RNA polymerase to direct expression of cloned genes.
1990,
Pubmed
Sturm,
The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.
1988,
Pubmed
Tada,
A novel sequence-specific DNA-binding protein, LCP-1, interacts with single-stranded DNA and differentially regulates early gene expression of the human neurotropic JC virus.
1992,
Pubmed
Tafuri,
Xenopus Y-box transcription factors: molecular cloning, functional analysis and developmental regulation.
1990,
Pubmed
Takimoto,
Specific binding of heterogeneous ribonucleoprotein particle protein K to the human c-myc promoter, in vitro.
1993,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Turner,
Leucine repeats and an adjacent DNA binding domain mediate the formation of functional cFos-cJun heterodimers.
1989,
Pubmed
Weinberger,
Identification of human glucocorticoid receptor complementary DNA clones by epitope selection.
1985,
Pubmed
Wu,
The locus of sequence-directed and protein-induced DNA bending.
1984,
Pubmed
Young,
Efficient isolation of genes by using antibody probes.
1983,
Pubmed
