Chemotherapy 2009 Jan 01;551:60-8. doi: 10.1159/000180340.

Antiproliferative, cytotoxic and hemolytic activities of a triterpene glycoside from Psolus patagonicus and its desulfated analog.

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BACKGROUND: The major triterpene glycoside of the sea cucumber Psolus patagonicus and its desulfated analog were tested for their antiproliferative, cytotoxic and hemolytic activities, and their effect on NF-kappaB activation. METHODS: The antiproliferative action of glycosides 1 and 2 were determined on 3 tumor cell lines. Their effect on the activation of NF-kappaB was evaluated by indirect immunofluorescence assay staining and the concomitant IkappaBalpha degradation was studied by Western blot. RESULTS: Both compounds were able to suppress the growth of 3 tumor cell lines (Hep3B, MDA-MB231 and A549) and induced the activation of NF-kappaB, a key player linking chronic inflammation and cancer, concomitant with IkappaBalpha degradation in the A549 tumor cell line. Compounds 1 and 2 showed hemolytic activity with half maximal inhibitory concentration (IC(50)) values around 80 microM. CONCLUSIONS: Both glycosides showed low cytotoxic activity in A549 tumor cells in comparison with sea cucumber triterpene glycosides containing a linear tetrasaccharide chain. This could be a result of the uncommon presence of two 12alpha- and 17alpha-hydroxyl groups and a Delta(7) double bond in the aglycone moiety. This aglycone functionalization may be related to their low membranolytic activity. Although glycosides 1 and 2 exert an antiproliferative effect, their mechanisms of action do not involve inhibition of NF-kappaB. Recently, it has been shown that diverse and new mechanisms of action are responsible for the antitumor and cytotoxic activities of marine compounds. Therefore, more extensive studies are needed to establish a mechanism of action and to deduce a clear structure-activity relationship of sea cucumber triterpene glycosides.

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Genes referenced: LOC100887844 LOC100893907 LOC115919910 LOC115921237