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J Biophys Biochem Cytol
1961 Aug 01;10:467-74. doi: 10.1083/jcb.10.4.467.
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The direct isolation of the mitotic apparatus.
MAZIA D
,
MITCHISON JM
,
MEDINA H
,
HARRIS P
.
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A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releasing the visibly intact mitotic apparatus. The method is designed for studies of enzyme activities, lipid components, and the variables affecting the stability of the apparatus.
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