Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Mol Cell Biol
1987 Dec 01;712:4238-46. doi: 10.1128/mcb.7.12.4238-4246.1987.
Show Gene links
Show Anatomy links
Stimulation of tubulin gene transcription by deciliation of sea urchin embryos.
Gong ZY
,
Brandhorst BP
.
Abstract
Deciliation by hypertonic shock of embryos of the sea urchin Lytechinus pictus resulted in an increase in synthesis of alpha- and beta-tubulins, the consequence of an increased concentration of RNA encoding the tubulins. RNA run-on assays in isolated nuclei indicated that this response is due to a transient increase in the rate of synthesis of tubulin RNA beginning within 5 min of deciliation. This enhancement of tubulin gene transcription also occurred in deciliated embryos treated with the microtubule-depolymerizing agent colcemid; thus the reaction to deciliation is not a response to a reduction in concentration of unpolymerized tubulin utilized for ciliogenesis. In deciliated embryos treated with colcemid, the elevated level of tubulin RNA declined rapidly, due to its destabilization by the elevated concentration of unpolymerized tubulin. The increased transcription of tubulin genes is a response to the loss of cilia, not to the hypertonic shock, and occurs even when cilium regeneration is prevented. Inhibition of protein synthesis with puromycin or emetine did not prevent the transcriptional enhancement but stabilized tubulin mRNA, resulting in increased accumulation of tubulin mRNA after deciliation.
Alexandraki,
Evolution of alpha q- and beta-tubulin genes as inferred by the nucleotide sequences of sea urchin cDNA clones.
1983, Pubmed,
Echinobase
Alexandraki,
Evolution of alpha q- and beta-tubulin genes as inferred by the nucleotide sequences of sea urchin cDNA clones.
1983,
Pubmed
,
Echinobase
Alexandraki,
Multiple polymorphic alpha- and beta-tubulin mRNAs are present in sea urchin eggs.
1985,
Pubmed
,
Echinobase
Alexandraki,
Expression of alpha- and beta-tubulin genes during development of sea urchin embryos.
1985,
Pubmed
,
Echinobase
Aronson,
Reversal by light of the action of N-methyl N-desacetyl colchicine on mitosis.
1970,
Pubmed
Auclair,
Cilia regeneration in the sea urchin embryo: evidence for a pool of ciliary proteins.
1966,
Pubmed
,
Echinobase
Baker,
Rapid changes in tubulin RNA synthesis and stability induced by deflagellation in Chlamydomonas.
1984,
Pubmed
Baker,
Protein synthesis is required for rapid degradation of tubulin mRNA and other deflagellation-induced RNAs in Chlamydomonas reinhardi.
1986,
Pubmed
Ben-Ze'ev,
Mechanisms of regulating tubulin synthesis in cultured mammalian cells.
1979,
Pubmed
Bibring,
Tubulin synthesis in sea urchin embryos: almost all tubulin of the first cleavage mitotic apparatus derives from the unfertilized egg.
1977,
Pubmed
,
Echinobase
Bibring,
Tubulin synthesis in sea urchin embryos. II. Ciliary A tubulin derives from the unfertilized egg.
1981,
Pubmed
,
Echinobase
Burns,
Kinetics of the regeneration of sea-urchin cilia.
1973,
Pubmed
,
Echinobase
Bédard,
Patterns of protein synthesis and metabolism during sea urchin embryogenesis.
1983,
Pubmed
,
Echinobase
Caron,
Autoregulation of tubulin synthesis in hepatocytes and fibroblasts.
1985,
Pubmed
Caron,
Autoregulation of tubulin synthesis in enucleated cells.
1985,
Pubmed
Cleveland,
Unpolymerized tubulin modulates the level of tubulin mRNAs.
1981,
Pubmed
Cleveland,
Is apparent autoregulatory control of tubulin synthesis nontranscriptionally regulated?
1983,
Pubmed
Cleveland,
Elevation of tubulin levels by microinjection suppresses new tubulin synthesis.
1983,
Pubmed
Conlon,
Post-transcriptional restriction of gene expression in sea urchin interspecies hybrid embryos.
1987,
Pubmed
,
Echinobase
GROSS,
MACROMOLECULE SYNTHESIS AND THE INFLUENCE OF ACTINOMYCIN ON EARLY DEVELOPMENT.
1964,
Pubmed
Greenhouse,
Sea urchin embryos are permeable to actinomycin.
1971,
Pubmed
,
Echinobase
Groudine,
Transcriptional regulation of hemoglobin switching in chicken embryos.
1981,
Pubmed
Harlow,
Developmental and tissue-specific regulation of beta-tubulin gene expression in the embryo of the sea urchin Strongylocentrotus purpuratus.
1987,
Pubmed
,
Echinobase
Keller,
Transcription of alpha- and beta-tubulin genes in vitro in isolated Chlamydomonas reinhardi nuclei.
1984,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lefebvre,
Flagellar elongation and shortening in Chlamydomonas. IV. Effects of flagellar detachment, regeneration, and resorption on the induction of flagellar protein synthesis.
1978,
Pubmed
Lefebvre,
Regulation of the synthesis and assembly of ciliary and flagellar proteins during regeneration.
1986,
Pubmed
Lehrach,
RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
1977,
Pubmed
Merlino,
Effects of deciliation of tubulin messenger RNA activity in sea urchin embryos.
1978,
Pubmed
,
Echinobase
Morris,
A factor in sea urchin eggs inhibits transcription in isolated nuclei by sea urchin RNA polymerase III.
1983,
Pubmed
,
Echinobase
O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed
Pittenger,
Retention of autoregulatory control of tubulin synthesis in cytoplasts: demonstration of a cytoplasmic mechanism that regulates the level of tubulin expression.
1985,
Pubmed
Raff,
Synthesis and storage of microtubule proteins by sea urchin embryos.
1971,
Pubmed
,
Echinobase
Rosenbaum,
Flagellar elongation and shortening in Chlamydomonas. The use of cycloheximide and colchicine to study the synthesis and assembly of flagellar proteins.
1969,
Pubmed
Schloss,
mRNA abundance changes during flagellar regeneration in Chlamydomonas reinhardtii.
1984,
Pubmed
Stephens,
Differential protein synthesis and utilization during cilia formation in sea urchin embryos.
1977,
Pubmed
,
Echinobase
Wahl,
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
1979,
Pubmed
Weeks,
Control of induction of tubulin synthesis in Chlamydomonas reinhardi.
1977,
Pubmed
Weeks,
Induction of microtubule protein synthesis in Chlamydomonas reinhardi during flagellar regeneration.
1976,
Pubmed
