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J Cell Biol
1970 Jun 01;453:606-14. doi: 10.1083/jcb.45.3.606.
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On the reconstitution of the crystalline components of the sea urchin fertilization membrane.
Bryan J
.
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The characteristics of the reconstitution of a crystalline component of the sea urchin fertilization membrane are presented. The reassembly of large aggregates of cylindrical or tubular components is effected by the addition of calcium or other divalent cations. The reassembly requires a slightly alkaline pH and is little affected by increasing ionic strength. Reassembly is strongly inhibited by treatment with reducing agents such as dithiothreitol. The role of this protein in the formation of the fertilization membrane and its possible relation to the calcium-insoluble proteins of the mitotic apparatus are discussed.
Bryan,
The isolation of a major structural element of the sea urchin fertilization membrane.
1970, Pubmed,
Echinobase
Bryan,
The isolation of a major structural element of the sea urchin fertilization membrane.
1970,
Pubmed
,
Echinobase
ENDO,
Changes in the cortical layer of sea urchin eggs at fertilization as studied with the electron microscope. I. Clypeaster japonicus.
1961,
Pubmed
,
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Good,
Hydrogen ion buffers for biological research.
1966,
Pubmed
HARRIS,
Electron microscope study of mitosis in sea urchin blastomeres.
1961,
Pubmed
,
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Inoue,
Fertilization membranes structure analysis with the surface replica method.
1967,
Pubmed
Ishihara,
An analysis of acid polysaccharides produced at fertilization of sea urchin.
1968,
Pubmed
,
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KANE,
The isolation and preliminary characterization of a major soluble protein of the sea urchin egg.
1959,
Pubmed
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Kane,
A comparative study of the isolation of the cortex and the role of the calcium-insoluble protein in several species of sea urchin egg.
1969,
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Echinobase
Kiefer,
The molecular unit of the microtubules of the mitotic apparatus.
1966,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
MAZIA,
The direct isolation of the mitotic apparatus.
1961,
Pubmed
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MEHL,
Acid and base production at fertilization in the sea urchin.
1961,
Pubmed
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McKenzie,
Milk proteins.
1967,
Pubmed
Sakai,
Studies on sulfhydryl groups during cell division of sea-urchin eggs. 8. Some properties of mitotic apparatus proteins.
1966,
Pubmed
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Echinobase
WOLPERT,
An electron microscope study of fertilisation of the sea urchin egg Psammechinus miliaris.
1961,
Pubmed
,
Echinobase
