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J Cell Biol
1984 May 01;985:1842-50. doi: 10.1083/jcb.98.5.1842.
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Monoclonal antibodies to dynein subunits reveal the existence of cytoplasmic antigens in sea urchin egg.
Piperno G
.
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Monoclonal antibodies directed against subunits of a sea urchin flagellar dynein were used to test for the presence of cytoplasmic antigens in preparations of fertilized eggs and mitotic apparati . A 9-10 S complex composed of 330,000-, 134,000-, and 126,000-mol-wt subunits was isolated from outer arms of Strongylocentrotus purpuratus sperm flagella and used to characterize the antibodies. Seven monospecific antibodies to the 330,000 subunit and two against the 134,000 subunit of the 9-10 S complex were identified by binding to nitrocellulose blots of electrophoretograms resolving polypeptides from different dynein preparations. The antibodies were applied also to blots of polypeptides from fertilized sea urchin egg at the first metaphase and a cellular fraction of mitotic apparati . Three of the antibodies to the 330,000 subunit bound to a cytoplasmic polypeptide of approximately the same molecular weight and the two antibodies to the smaller subunits recognized a polypeptide of 124,000 apparent molecular weight. Both antigens appeared to be enriched in the fraction containing mitotic apparati . These results indicate that polypeptides similar to two subunits of the 9-10 S complex are present in eggs at metaphase, and they are apparently associated with the mitotic apparatus.
Bell,
Polypeptide subunits of dynein 1 from sea urchin sperm flagella.
1979, Pubmed,
Echinobase
Bell,
Polypeptide subunits of dynein 1 from sea urchin sperm flagella.
1979,
Pubmed
,
Echinobase
Cande,
Chromosome movement in lysed mitotic cells is inhibited by vanadate.
1978,
Pubmed
Gibbons,
A latent adenosine triphosphatase form of dynein 1 from sea urchin sperm flagella.
1979,
Pubmed
,
Echinobase
Gibbons,
Relationship between the latent adenosine triphosphatase state of dynein 1 and its ability to recombine functionally with KCl-extracted sea urchin sperm flagella.
1979,
Pubmed
,
Echinobase
HUNTER,
Preparation of iodine-131 labelled human growth hormone of high specific activity.
1962,
Pubmed
Haimo,
Dynein binds to and crossbridges cytoplasmic microtubules.
1979,
Pubmed
Hisanaga,
Cytoplasmic dynein of the sea urchin egg. II. Purification, characterization and interactions with microtubules and Ca-calmodulin.
1983,
Pubmed
,
Echinobase
Huang,
Paralyzed flagella mutants of Chlamydomonas reinhardtii. Defective for axonemal doublet microtubule arms.
1979,
Pubmed
Klee,
Calmodulin.
1980,
Pubmed
Mabuchi,
ATPase in the cortical layer of sea urchin egg: its properties and interaction with cortex protein.
1973,
Pubmed
,
Echinobase
McIntosh,
Structure and physiology of the mammalian mitotic spindle.
1975,
Pubmed
Piperno,
Microtubular proteins of Chlamydomonas reinhardtii. An immunochemical study based on the use of an antibody specific for the beta-tubulin subunit.
1977,
Pubmed
Piperno,
Axonemal adenosine triphosphatases from flagella of Chlamydomonas reinhardtii. Purification of two dyneins.
1979,
Pubmed
Piperno,
Inner arm dyneins from flagella of Chlamydomonas reinhardtii.
1981,
Pubmed
Pratt,
The identification of a dynein ATPase in unfertilized sea urchin eggs.
1980,
Pubmed
,
Echinobase
Pratt,
Dynein-like Mg2+-ATPase in mitotic spindles isolated from sea urchin embryos (Strongylocentrotus droebachiensis).
1980,
Pubmed
,
Echinobase
Salmon,
Calcium-labile mitotic spindles isolated from sea urchin eggs (Lytechinus variegatus).
1980,
Pubmed
,
Echinobase
Simons,
Vanadate--a new tool for biologists.
1979,
Pubmed
Tang,
Structure of the dynein-1 outer arm in sea urchin sperm flagella. I. Analysis by separation of subunits.
1982,
Pubmed
,
Echinobase
Telzer,
Decoration of spindle microtubules with Dynein: evidence for uniform polarity.
1981,
Pubmed
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
de StGroth,
Production of monoclonal antibodies: strategy and tactics.
1980,
Pubmed