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Mol Biol Cell
2006 Dec 01;1712:5241-52. doi: 10.1091/mbc.e06-07-0634.
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Rendezvin: An essential gene encoding independent, differentially secreted egg proteins that organize the fertilization envelope proteome after self-association.
Wong JL
,
Wessel GM
.
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Preventing polyspermy during animal fertilization relies on modifications to the egg''s extracellular matrix. On fertilization in sea urchins, the contents of cortical granules are secreted and rapidly assemble into the egg''s extracellular vitelline layer, forming the fertilization envelope, a proteinaceous structure that protects the zygote from subsequent sperm. Here, we document rendezvin, a gene whose transcript is differentially spliced to yield proteins destined for either cortical granules or the vitelline layer. These distinctly trafficked variants reunite after cortical granule secretion at fertilization. Together, they help coordinate assembly of the functional fertilization envelope, whose proteome is now defined in full.
Andersen,
Identification of the minimal functional unit in the low density lipoprotein receptor-related protein for binding the receptor-associated protein (RAP). A conserved acidic residue in the complement-type repeats is important for recognition of RAP.
2000, Pubmed
Andersen,
Identification of the minimal functional unit in the low density lipoprotein receptor-related protein for binding the receptor-associated protein (RAP). A conserved acidic residue in the complement-type repeats is important for recognition of RAP.
2000,
Pubmed
Anderson,
Oocyte differentiation in the sea urchin, Arbacia punctulata, with particular reference to the origin of cortical granules and their participation in the cortical reaction.
1968,
Pubmed
,
Echinobase
Bisgrove,
The SpEGF III gene encodes a member of the fibropellins: EGF repeat-containing proteins that form the apical lamina of the sea urchin embryo.
1993,
Pubmed
,
Echinobase
Bork,
The CUB domain. A widespread module in developmentally regulated proteins.
1993,
Pubmed
Bruskin,
Accumulation in embryogenesis of five mRNAs enriched in the ectoderm of the sea urchin pluteus.
1981,
Pubmed
,
Echinobase
Calvete,
Boar spermadhesins AQN-1 and AQN-3: oligosaccharide and zona pellucida binding characteristics.
1996,
Pubmed
Carroll,
A novel procedure for obtaining denuded sea urchin eggs and observations on the role of the vitelline layer in sperm reception and egg activation.
1977,
Pubmed
,
Echinobase
Carroll,
Structure, assembly and function of the surface envelope (fertilization envelope) from eggs of the sea urchin, Strongylocentrotus purpuratus.
1986,
Pubmed
,
Echinobase
Correa,
Characterization of the vitelline envelope of the sea urchin Strongylocentrotus purpuratus.
1997,
Pubmed
,
Echinobase
Crabb,
In vitro reconstitution of exocytosis from plasma membrane and isolated secretory vesicles.
1985,
Pubmed
,
Echinobase
Detering,
Isolation and characterization of plasma membrane-associated cortical granules from sea urchin eggs.
1977,
Pubmed
,
Echinobase
Dostálová,
Boar spermadhesin AWN-1. Oligosaccharide and zona pellucida binding characteristics.
1995,
Pubmed
Engler-Blum,
Reduction of background problems in nonradioactive northern and Southern blot analyses enables higher sensitivity than 32P-based hybridizations.
1993,
Pubmed
Epel,
Methods for revoval of the vitelline membrane of sea urchin eggs. I. Use of dithiothreitol (Cleland Reagent).
1970,
Pubmed
,
Echinobase
Esser,
Mutational analysis of the ligand binding domain of the low density lipoprotein receptor.
1988,
Pubmed
Ettensohn,
Cell lineage conversion in the sea urchin embryo.
1988,
Pubmed
,
Echinobase
Gache,
Monoclonal antibodies to the sea urchin egg vitelline layer inhibit fertilization by blocking sperm adhesion.
1983,
Pubmed
,
Echinobase
Glabe,
Isolation and characterization of the vitelline layer of sea urchin eggs.
1977,
Pubmed
,
Echinobase
Guo,
The role of a conserved acidic residue in calcium-dependent protein folding for a low density lipoprotein (LDL)-A module: implications in structure and function for the LDL receptor superfamily.
2004,
Pubmed
Haley,
Proteolytic cleavage of the cell surface protein p160 is required for detachment of the fertilization envelope in the sea urchin.
2004,
Pubmed
,
Echinobase
Kamei,
The species-specific egg receptor for sea urchin sperm adhesion is EBR1,a novel ADAMTS protein.
2003,
Pubmed
,
Echinobase
Kinsey,
Purification and properties of the egg plasma membrane.
1986,
Pubmed
,
Echinobase
Kodama,
cDNA cloning and functional analysis of ascidian sperm proacrosin.
2001,
Pubmed
Laitinen,
Mutation of a critical tryptophan to lysine in avidin or streptavidin may explain why sea urchin fibropellin adopts an avidin-like domain.
1999,
Pubmed
,
Echinobase
Lyons,
Expression and structural analysis of a teleost homolog of a mammalian zona pellucida gene.
1993,
Pubmed
McGadey,
A tetrazolium method for non-specific alkaline phosphatase.
1970,
Pubmed
Niman,
Proteins of the sea urchin egg vitelline layer.
1984,
Pubmed
,
Echinobase
Ransick,
Whole mount in situ hybridization shows Endo 16 to be a marker for the vegetal plate territory in sea urchin embryos.
1993,
Pubmed
,
Echinobase
Romero,
The crystal structures of two spermadhesins reveal the CUB domain fold.
1997,
Pubmed
Rong,
Conversion of a human low-density lipoprotein receptor ligandbinding repeat to a virus receptor: identification of residues important for ligand specificity.
1998,
Pubmed
Russell,
Different combinations of cysteine-rich repeats mediate binding of low density lipoprotein receptor to two different proteins.
1989,
Pubmed
Smith,
Comparative variation of morphological and molecular evolution through geologic time: 28S ribosomal RNA versus morphology in echinoids.
1992,
Pubmed
,
Echinobase
Somers,
Localization and developmental fate of ovoperoxidase and proteoliaisin, two proteins involved in fertilization envelope assembly.
1989,
Pubmed
,
Echinobase
Somers,
The heme environment of ovoperoxidase as determined by optical spectroscopy.
1989,
Pubmed
,
Echinobase
Somers,
Functional domains of proteoliaisin, the adhesive protein that orchestrates fertilization envelope assembly.
1991,
Pubmed
,
Echinobase
Song,
Oogenesis: single cell development and differentiation.
2006,
Pubmed
,
Echinobase
Spurr,
A low-viscosity epoxy resin embedding medium for electron microscopy.
1969,
Pubmed
Sugiyama,
A novel low-density lipoprotein receptor-related protein mediating cellular uptake of apolipoprotein E-enriched beta-VLDL in vitro.
2000,
Pubmed
Töpfer-Petersen,
Spermadhesins: a new protein family. Facts, hypotheses and perspectives.
1998,
Pubmed
Töpfer-Petersen,
Oocyte-sperm interactions.
2000,
Pubmed
Varela,
The 2.4 A resolution crystal structure of boar seminal plasma PSP-I/PSP-II: a zona pellucida-binding glycoprotein heterodimer of the spermadhesin family built by a CUB domain architecture.
1997,
Pubmed
Villacorta-Moeller,
Sea urchin embryo fertilization envelope: immunological evidence that soluble envelope proteins are derived from cortical granule secretions.
1982,
Pubmed
,
Echinobase
Weidman,
Assembly of the sea urchin fertilization membrane: isolation of proteoliaisin, a calcium-dependent ovoperoxidase binding protein.
1985,
Pubmed
,
Echinobase
Weidman,
Regulation of extracellular matrix assembly: in vitro reconstitution of a partial fertilization envelope from isolated components.
1987,
Pubmed
,
Echinobase
Wessel,
SFE1, a constituent of the fertilization envelope in the sea urchin is made by oocytes and contains low-density lipoprotein-receptor-like repeats.
2000,
Pubmed
,
Echinobase
Wessel,
The biology of cortical granules.
2001,
Pubmed
Wessel,
Ontogeny of the basal lamina in the sea urchin embryo.
1984,
Pubmed
,
Echinobase
Wessel,
A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids.
1984,
Pubmed
Wessel,
Gastrulation in the sea urchin is accompanied by the accumulation of an endoderm-specific mRNA.
1989,
Pubmed
,
Echinobase
Wessel,
A protein of the sea urchin cortical granules is targeted to the fertilization envelope and contains an LDL-receptor-like motif.
1995,
Pubmed
,
Echinobase
Wong,
Major components of a sea urchin block to polyspermy are structurally and functionally conserved.
2004,
Pubmed
,
Echinobase
Wong,
Defending the zygote: search for the ancestral animal block to polyspermy.
2006,
Pubmed
Wong,
The oxidative burst at fertilization is dependent upon activation of the dual oxidase Udx1.
2004,
Pubmed
,
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