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Poisson-distributed active fusion complexes underlie the control of the rate and extent of exocytosis by calcium.
Vogel SS
,
Blank PS
,
Zimmerberg J
.
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We have investigated the consequences of having multiple fusion complexes on exocytotic granules, and have identified a new principle for interpreting the calcium dependence of calcium-triggered exocytosis. Strikingly different physiological responses to calcium are expected when active fusion complexes are distributed between granules in a deterministic or probabilistic manner. We have modeled these differences, and compared them with the calcium dependence of sea urchin egg cortical granule exocytosis. From the calcium dependence of cortical granule exocytosis, and from the exposure time and concentration dependence of N-ethylmaleimide inhibition, we determined that cortical granules do have spare active fusion complexes that are randomly distributed as a Poisson process among the population of granules. At high calcium concentrations, docking sites have on average nine active fusion complexes.
Adler,
Alien intracellular calcium chelators attenuate neurotransmitter release at the squid giant synapse.
1991, Pubmed
Adler,
Alien intracellular calcium chelators attenuate neurotransmitter release at the squid giant synapse.
1991,
Pubmed
Almers,
Synapses. How fast can you get?
1994,
Pubmed
Augustine,
Calcium requirements for secretion in bovine chromaffin cells.
1992,
Pubmed
Baker,
Secretion-dependent uptake of extracellular fluid by the rat neurohypophysis.
1974,
Pubmed
Baker,
Calcium-dependence of the cortical reaction in broken eggs of Echinus esculentus [proceedings].
1978,
Pubmed
,
Echinobase
Bennett,
A molecular description of synaptic vesicle membrane trafficking.
1994,
Pubmed
Betz,
Activity-dependent fluorescent staining and destaining of living vertebrate motor nerve terminals.
1992,
Pubmed
Chandler,
Exocytosis in vitro: ultrastructure of the isolated sea urchin egg cortex as seen in platinum replicas.
1984,
Pubmed
,
Echinobase
Chow,
Time course of Ca2+ concentration triggering exocytosis in neuroendocrine cells.
1994,
Pubmed
Crabb,
Polycation inhibition of exocytosis from sea urchin egg cortex.
1986,
Pubmed
,
Echinobase
Douglas,
Involvement of calcium in exocytosis and the exocytosis--vesiculation sequence.
1974,
Pubmed
Ferro-Novick,
Vesicle fusion from yeast to man.
1994,
Pubmed
Geppert,
Synaptotagmin I: a major Ca2+ sensor for transmitter release at a central synapse.
1994,
Pubmed
Guraya,
Recent progress in the structure, origin, composition, and function of cortical granules in animal egg.
1982,
Pubmed
Haggerty,
Release of granule contents from sea urchin egg cortices. New assay procedures and inhibition by sulfhydryl-modifying reagents.
1983,
Pubmed
,
Echinobase
Hammel,
Theoretical considerations on the formation of secretory granules in the rat pancreas.
1993,
Pubmed
Hammel,
Studies on the growth of mast cells in rats. Changes in granule size between 1 and 6 months.
1988,
Pubmed
Heidelberger,
Calcium dependence of the rate of exocytosis in a synaptic terminal.
1994,
Pubmed
Heinemann,
A two-step model of secretion control in neuroendocrine cells.
1993,
Pubmed
Heuser,
Structural changes after transmitter release at the frog neuromuscular junction.
1981,
Pubmed
Holz,
MgATP-independent and MgATP-dependent exocytosis. Evidence that MgATP primes adrenal chromaffin cells to undergo exocytosis.
1989,
Pubmed
Horrigan,
Releasable pools and the kinetics of exocytosis in adrenal chromaffin cells.
1994,
Pubmed
Jackson,
Mild proteolytic digestion restores exocytotic activity to N-ethylmaleimide-inactivated cell surface complex from sea urchin eggs.
1985,
Pubmed
,
Echinobase
Jaffe,
Electrical regulation of sperm-egg fusion.
1986,
Pubmed
,
Echinobase
Kaplan,
Single cell fusion events induced by influenza hemagglutinin: studies with rapid-flow, quantitative fluorescence microscopy.
1991,
Pubmed
Katz,
Quantal mechanism of neural transmitter release.
1971,
Pubmed
Knight,
Calcium-dependence of catecholamine release from bovine adrenal medullary cells after exposure to intense electric fields.
1982,
Pubmed
Knight,
Exocytosis from the vesicle viewpoint: an overview.
1987,
Pubmed
Kono,
The relationship between the insulin-binding capacity of fat cells and the cellular response to insulin. Studies with intact and trypsin-treated fat cells.
1971,
Pubmed
Koshland,
Comparison of experimental binding data and theoretical models in proteins containing subunits.
1966,
Pubmed
Llinás,
Microdomains of high calcium concentration in a presynaptic terminal.
1992,
Pubmed
MONOD,
ON THE NATURE OF ALLOSTERIC TRANSITIONS: A PLAUSIBLE MODEL.
1965,
Pubmed
McLaughlin,
Cations that alter surface potentials of lipid bilayers increase the calcium requirement for exocytosis in sea urchin eggs.
1988,
Pubmed
,
Echinobase
Mohri,
Quantitative analysis of the process and propagation of cortical granule breakdown in sea urchin eggs.
1990,
Pubmed
,
Echinobase
Mohri,
Propagation of transient Ca2+ increase in sea urchin eggs upon fertilization and its regulation by microinjecting EGTA solution.
1991,
Pubmed
,
Echinobase
NICKERSON,
Receptor occupancy and tissue response.
1956,
Pubmed
Neher,
Mice sans synaptotagmin.
1994,
Pubmed
Neher,
Multiple calcium-dependent processes related to secretion in bovine chromaffin cells.
1993,
Pubmed
Neubig,
Permeability control by cholinergic receptors in Torpedo postsynaptic membranes: agonist dose-response relations measured at second and millisecond times.
1980,
Pubmed
Roberts,
Colocalization of ion channels involved in frequency selectivity and synaptic transmission at presynaptic active zones of hair cells.
1990,
Pubmed
Rothman,
Mechanisms of intracellular protein transport.
1994,
Pubmed
STEPHENSON,
A modification of receptor theory.
1956,
Pubmed
Thomas,
A low affinity Ca2+ receptor controls the final steps in peptide secretion from pituitary melanotrophs.
1993,
Pubmed
Thomas,
A triggered mechanism retrieves membrane in seconds after Ca(2+)-stimulated exocytosis in single pituitary cells.
1994,
Pubmed
Thomas,
Cytosolic Ca2+, exocytosis, and endocytosis in single melanotrophs of the rat pituitary.
1990,
Pubmed
Vacquier,
The isolation of intact cortical granules from sea urchin eggs: calcium lons trigger granule discharge.
1975,
Pubmed
,
Echinobase
Vogel,
Calcium-triggered fusion of exocytotic granules requires proteins in only one membrane.
1992,
Pubmed
,
Echinobase
Vogel,
Lysophosphatidylcholine reversibly arrests exocytosis and viral fusion at a stage between triggering and membrane merger.
1993,
Pubmed
,
Echinobase
Vogel,
Application of a membrane fusion assay for rapid drug screening.
1995,
Pubmed
,
Echinobase
Vogel,
The sea urchin cortical reaction. A model system for studying the final steps of calcium-triggered vesicle fusion.
1991,
Pubmed
,
Echinobase
Vogel,
Proteins on exocytic vesicles mediate calcium-triggered fusion.
1992,
Pubmed
,
Echinobase
Whalley,
The N-ethylmaleimide-sensitive protein thiol groups necessary for sea-urchin egg cortical-granule exocytosis are highly exposed to the medium and are required for triggering by Ca2+.
1994,
Pubmed
,
Echinobase
Whalley,
Direct membrane retrieval into large vesicles after exocytosis in sea urchin eggs.
1995,
Pubmed
,
Echinobase
Zimmerberg,
Exocytosis of sea urchin egg cortical vesicles in vitro is retarded by hyperosmotic sucrose: kinetics of fusion monitored by quantitative light-scattering microscopy.
1985,
Pubmed
,
Echinobase
von Rüden,
A Ca-dependent early step in the release of catecholamines from adrenal chromaffin cells.
1993,
Pubmed
