Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Nuclear membrane fusion in fertilized Lytechinus variegatus eggs.
Aronson JF
.
???displayArticle.abstract???
Fusion of apposed nuclear envelopes is frequently seen at telophase during postmitotic reorganization of the nucleus, but only rarely at other times in the cell cycle. We attempted to define an experimental system for studying changes in the nuclear envelope related to the cell cycle by varying the time of pronuclear apposition in fertilized Lytechinus variegatus eggs. This approach was based on the assumption that the period from fertilization to metaphase of the first cleavage division corresponds to the period from telophase to metaphase in the generalized cell cycle. The experimental approach used was to block the movement of the pronuclei with Colcemid and then to release this block at varying times after insemination by photochemically inactivating the Colcemid. The results show that apposed pronuclear envelopes can fuse from soon after insemination until the anticipated time of prometaphase. Fusion occurred in about 3 min as scored by light microscopy and this time did not vary significantly with the time after insemination. The potential for nuclear fusion is not restricted to pronuclei alone since diploid nuclei in binucleate cells could be fused using centrifugation in solutions of Colcemid to bring the nuclei into apposition. It is suggested that the potential for nuclear fusion is not necessarily related to the cell cycle and that modification of the nuclear envelope, possibly by association with chromatin or other fibrous material restricts nuclear fusion in most multinucleated cells.
ALLEN,
Fertilization and activation of sea urchin eggs in glass capillaries. I. Membrane elevation and nuclear movements in totally and partially fertilized eggs.
1954, Pubmed,
Echinobase
ALLEN,
Fertilization and activation of sea urchin eggs in glass capillaries. I. Membrane elevation and nuclear movements in totally and partially fertilized eggs.
1954,
Pubmed
,
Echinobase
Aronson,
Reversal by light of the action of N-methyl N-desacetyl colchicine on mitosis.
1970,
Pubmed
BRIGGS,
An investigation of the capacity for cleavage and differentiation in Rana pipiens eggs lacking "functional" chromosomes.
1951,
Pubmed
Borisy,
The mechanism of action of colchicine. Colchicine binding to sea urchin eggs and the mitotic apparatus.
1967,
Pubmed
,
Echinobase
DETTLAFF,
THE R OLE OF THE GERMINAL VESICLE IN OOECYTE MATURATION IN ANURANS AS REVEALED BY THE REMOVAL AND TRANSPLANTATION OF NUCLEI.
1964,
Pubmed
Fansler,
Sea urchin nuclear DNA polymerase. II. Changing localization during early development.
1969,
Pubmed
,
Echinobase
HINEGARDNER,
THE DNA SYNTHETIC PERIOD DURING EARLY DEVELOPMENT OF THE SEA URCHIN EGG.
1964,
Pubmed
,
Echinobase
Harris,
Artificial heterokaryons of animal cells from different species.
1966,
Pubmed
Herzog,
The localization of endogenous peroxidase in the lacrimal gland of the rat during postnatal development. Electron microscope cytochemical and biochemical studies.
1972,
Pubmed
JENSEN,
OBSERVATIONS ON THE FUSION OF NUCLEI IN PLANTS.
1964,
Pubmed
Jenkins,
Fine structure of division in ciliate protozoa. I. Micronuclear mitosis in Blepharisma.
1967,
Pubmed
Kilarski,
The formation of multivesicular bodies from the nuclear envelope.
1970,
Pubmed
Longo,
The fine structure of pronuclear development and fusion in the sea urchin, Arbacia punctulata.
1968,
Pubmed
,
Echinobase
Longo,
An ultrastructural analysis of fertilization in the surf clam, Spisula solidissima. II. Development of the male pronucleus and the association of the maternally and paternally derived chromosomes.
1970,
Pubmed
Rustad,
Nuclear-cytoplasmic relations in the mitosis of sea urchin eggs. II. The devision times of whole eggs and haploid and diploid half-eggs.
1970,
Pubmed
,
Echinobase
Urban,
The fine structure of pronuclear fusion in the coenocytic marine alga Bryopsis hypnoides Lamouroux.
1969,
Pubmed
Wigglesworth,
Polyploidy and nuclear fusion in the fat body of Rhodnius (Hemiptera).
1967,
Pubmed
Zimmerman,
Action of colcemid in sea urchin eggs.
1967,
Pubmed
,
Echinobase
