Li X , Wang C , Li N , Gao Y , Ju Z , Liao G , Xiong D .

	Figure 1. Experimental design of sea cucumber following exposure to elevated temperature (26 °C, Group 2), water-accommodated fractions (WAF) of Oman crude oil at an optimal temperature of 16 °C (WAF + 16 °C, Group 3), and Oman crude oil WAF at an elevated temperature of 26 °C (WAF + 26 °C, Group 4) for 24 h. The Control (Group 1) was the sea cucumber exposed to pre-filtered natural seawater only at an optimal temperature of 16 °C (n = 15).
	Figure 2. The concentrations of total petroleum hydrocarbons (TPH, (A)), the levels of US EPA’s 16 priority polycyclic aromatic hydrocarbons (ΣPAHs, (B)), and the proportion (%) of each PAH (C) in the water-accommodated fractions (WAF) of Oman crude oil at an optimal temperature of 16 °C (WAF + 16 °C, light-red filled) and Oman crude oil WAF at an elevated temperature of 26 °C (WAF + 26 °C, light-yellow filled) solutions. Nap: Naphthalene, Acy: Acenaphthylene, Ace: Acenaphthene, Fle: Fluorene, Phe: Phenanthrene, Ant: Anthracene, Fla: Fluoranthene, Pyr: Pyrene, B[a]A: Benzo[a]anthracene, Chr: Chrysene, B[b]F: Benzo[b]fuoranthene, B[k]F: Benzo[k]fuoranthene, B[a]P: Benzo[a]pyrene, I[123-cd]P: Indeno[1,2,3-cd]pyrene, D[ah]A: Dibenzo[a,h]anthracene, and B[ghi]P: Benzo[g,h,i]perylene.
	Figure 3. Reactive oxygen species (ROS) levels in the body wall of sea cucumber following exposure to elevated temperature (26 °C, light-blue filled square), water-accommodated fractions (WAF) of Oman crude oil at an optimal temperature of 16 °C (WAF + 16 °C, light-red filled up-triangle), and Oman crude oil WAF at an elevated temperature of 26 °C (WAF + 26 °C, light-yellow filled down-triangle). The Control was the sea cucumber exposed to pre-filtered natural seawater only at an optimal temperature of 16 °C (light-green filled circle). Error bars represent 95% confidence intervals. Asterisks (**, or ***) denote the significant differences between the treatments and the Control (p < 0.01, or 0.001, respectively). Dark traits denote the significant differences among the treatments (two-way analysis of variance (ANOVA)).
	Figure 4. Total antioxidant capacity (T-AOC) in the body wall of sea cucumber following exposure to elevated temperature (26 °C, light-blue filled), water-accommodated fractions (WAF) of Oman crude oil at an optimal temperature of 16 °C (WAF + 16 °C, light-red filled), and Oman crude oil WAF at an elevated temperature of 26 °C (WAF + 26 °C, light-yellow filled). The Control was the sea cucumber exposed to pre-filtered natural seawater only at an optimal temperature of 16 °C (light-green filled). Asterisks (* or **) denote the significant differences between the treatments and the Control (p < 0.05 or 0.01, respectively). Dark traits denote the significant differences among the treatments (two-way analysis of variance (ANOVA)).
	Figure 5. The levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG) (A), protein carbonyls (PCO) (B), and malondialdehyde (MDA) (C) in the body wall of sea cucumber following exposure to elevated temperature (26 °C, light-blue filled), water-accommodated fractions (WAF) of Oman crude oil at an optimal temperature of 16 °C (WAF + 16 °C, light-red filled), and Oman crude oil WAF at an elevated temperature of 26 °C (WAF + 26 °C, light-yellow filled). The Control was the sea cucumber exposed to pre-filtered natural seawater only at an optimal temperature of 16 °C (light-green filled). Asterisks (*, **, or ***) denote the significant differences between the treatments and the Control (p < 0.05, 0.01, or 0.001, respectively). Dark traits denote the significant differences among the treatments (two-way analysis of variance (ANOVA)).
	Figure 6. Relative caspase-3 activity (fold) in the body wall of sea cucumber following exposure to elevated temperature (26 °C, light-blue filled square), water-accommodated fractions (WAF) of Oman crude oil at an optimal temperature of 16 °C (WAF + 16 °C, light-red filled up-triangle), and Oman crude oil WAF at an elevated temperature of 26 °C (WAF + 26 °C, light-yellow filled down-triangle). The Control was the sea cucumber exposed to pre-filtered natural seawater only at an optimal temperature of 16 °C (light-green filled circle). Error bars represent 95% confidence intervals. Asterisks ***) denote the significant differences between the treatments and the Control (p < 0.001, respectively). Dark traits denote the significant differences among the treatments (two-way analysis of variance (ANOVA)).
	Figure 7. Integrated biomarker response (IBR) indexes for sea cucumber following exposure to elevated temperature (26 °C, light-blue filled, (A), water-accommodated fractions (WAF) of Oman crude oil at an optimal temperature of 16 °C (WAF + 16 °C, light-red filled, (B), and Oman crude oil WAF at an elevated temperature of 26 °C (WAF + 26 °C, light-yellow filled, (C). Biomarker results are denoted relative to the Control (sea cucumber exposed to pre-filtered natural seawater only at an optimal temperature of 16 °C) (black dash lines). The area above 0 denotes the induction of the biomarker, and the area below 0 denotes the reduction of the biomarkers. ROS: Reactive oxygen species; T-AOC: Total antioxidant capacity; 8-OHdG: 8-hydroxy-2′-deoxyguanosine; PCO: Protein carbonyls; MDA: Malondialdehyde.

Ahamed, Co-Exposure to SiO2 Nanoparticles and Arsenic Induced Augmentation of Oxidative Stress and Mitochondria-Dependent Apoptosis in Human Cells. 2019, Pubmed

Ahamed, Co-Exposure to SiO2 Nanoparticles and Arsenic Induced Augmentation of Oxidative Stress and Mitochondria-Dependent Apoptosis in Human Cells. 2019, Pubmed
Ahmed, Global Aquaculture Productivity, Environmental Sustainability, and Climate Change Adaptability. 2019, Pubmed
Alarifi, Oxidative stress contributes to cobalt oxide nanoparticles-induced cytotoxicity and DNA damage in human hepatocarcinoma cells. 2013, Pubmed
Andersen, Effects of crude oil exposure and elevated temperature on the liver transcriptome of polar cod (Boreogadus saida). 2015, Pubmed
Barbosa, Acute exposure to water-soluble fractions of marine diesel oil: Evaluation of apoptosis and oxidative stress in an ascidian. 2018, Pubmed
Baum, Metabolic performance of the coral reef fish Siganus guttatus exposed to combinations of water borne diesel, an anionic surfactant and elevated temperature in Indonesia. 2016, Pubmed
Beliaeff, Integrated biomarker response: a useful tool for ecological risk assessment. 2002, Pubmed
Benzie, The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. 1996, Pubmed
Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. 1976, Pubmed
Braga, Cytotoxicity and enzymatic biomarkers as early indicators of benthic responses to the soluble-fraction of diesel oil. 2018, Pubmed
Brander, Global fish production and climate change. 2007, Pubmed
Buege, Microsomal lipid peroxidation. 1978, Pubmed
Cui, Effects of aerial exposure on oxidative stress, antioxidant and non-specific immune responses of juvenile sea cucumber Apostichopus japonicus under low temperature. 2020, Pubmed , Echinobase
Di Meo, Role of ROS and RNS Sources in Physiological and Pathological Conditions. 2016, Pubmed
Ding, Transcriptome analysis provides insights into the molecular mechanisms responsible for evisceration behavior in the sea cucumber Apostichopus japonicus. 2019, Pubmed , Echinobase
Duan, Transgenerational effects of heavy fuel oil on the sea urchin Strongylocentrotus intermedius considering oxidative stress biomarkers. 2018, Pubmed , Echinobase
Duan, Parental exposure to heavy fuel oil induces developmental toxicity in offspring of the sea urchin Strongylocentrotus intermedius. 2018, Pubmed , Echinobase
Han, Effects of water accommodated fractions (WAFs) of crude oil in two congeneric copepods Tigriopus sp. 2017, Pubmed
Hannam, Immunotoxicity and oxidative stress in the Arctic scallop Chlamys islandica: effects of acute oil exposure. 2010, Pubmed
Hengartner, The biochemistry of apoptosis. 2000, Pubmed
Hodson, The Toxicity to Fish Embryos of PAH in Crude and Refined Oils. 2017, Pubmed
Huo, Global-warming-caused changes of temperature and oxygen alter the proteomic profile of sea cucumber Apostichopus japonicus. 2019, Pubmed , Echinobase
Huo, Metabolome responses of the sea cucumber Apostichopus japonicus to multiple environmental stresses: Heat and hypoxia. 2019, Pubmed , Echinobase
Huo, Time course analysis of immunity-related gene expression in the sea cucumber Apostichopus japonicus during exposure to thermal and hypoxic stress. 2019, Pubmed , Echinobase
Incardona, Molecular Mechanisms of Crude Oil Developmental Toxicity in Fish. 2017, Pubmed
Incardona, Developmental toxicity of 4-ring polycyclic aromatic hydrocarbons in zebrafish is differentially dependent on AH receptor isoforms and hepatic cytochrome P4501A metabolism. 2006, Pubmed
Jia, Effects of nitrite exposure on haematological parameters, oxidative stress and apoptosis in juvenile turbot (Scophthalmus maximus). 2015, Pubmed
Jiang, Oxidative Stress in Shellfish Sinonovacula constricta Exposed to the Water Accommodated Fraction of Zero Sulfur Diesel Oil and Pinghu Crude Oil. 2017, Pubmed
Johnstone, Effects of elevated temperature on gonadal functions, cellular apoptosis, and oxidative stress in Atlantic sea urchin Arbacia punculata. 2019, Pubmed , Echinobase
Kamyab, Effects of thermal stress on the immune and oxidative stress responses of juvenile sea cucumber Holothuria scabra. 2017, Pubmed , Echinobase
LeBel, Evaluation of the probe 2',7'-dichlorofluorescin as an indicator of reactive oxygen species formation and oxidative stress. 1992, Pubmed
Lee, Acute and chronic toxicity study of the water accommodated fraction (WAF), chemically enhanced WAF (CEWAF) of crude oil and dispersant in the rock pool copepod Tigriopus japonicus. 2013, Pubmed
Lee, Exposure to ZnO/TiO2 Nanoparticles Affects Health Outcomes in Cosmetics Salesclerks. 2020, Pubmed
Levine, Carbonyl assays for determination of oxidatively modified proteins. 1994, Pubmed
Li, Effect of seasonal high temperature on the immune response in Apostichopus japonicus by transcriptome analysis. 2019, Pubmed , Echinobase
Li, Exposure to water-accommodated fractions of two different crude oils alters morphology, cardiac function and swim bladder development in early-life stages of zebrafish. 2019, Pubmed
Li, Toxicity of Water-Accommodated Fractions (WAF), Chemically Enhanced WAF (CEWAF) of Oman Crude Oil and Dispersant to Early-Life Stages of Zebrafish (Danio rerio). 2018, Pubmed
Li, Phenotypic and transcriptomic consequences in zebrafish early-life stages following exposure to crude oil and chemical dispersant at sublethal concentrations. 2021, Pubmed
Liang, Insights into high-pressure acclimation: comparative transcriptome analysis of sea cucumber Apostichopus japonicus at different hydrostatic pressure exposures. 2020, Pubmed , Echinobase
Liu, The Impact of the Deepwater Horizon Oil Spill upon Lung Health-Mouse Model-Based RNA-Seq Analyses. 2020, Pubmed
Liu, Metabolites analysis, metabolic enzyme activities and bioaccumulation in the clam Ruditapes philippinarum exposed to benzo[a]pyrene. 2014, Pubmed
Lyons, The influence of water temperature on induced liver EROD activity in Atlantic cod (Gadus morhua) exposed to crude oil and oil dispersants. 2011, Pubmed
Marissen, Identification of caspase 3-mediated cleavage and functional alteration of eukaryotic initiation factor 2alpha in apoptosis. 2000, Pubmed
Matos, Toxicity Evaluation of Quantum Dots (ZnS and CdS) Singly and Combined in Zebrafish (Danio rerio). 2019, Pubmed
Morroni, Sea cucumber Holothuria polii (Delle Chiaje, 1823) as new model for embryo bioassays in ecotoxicological studies. 2020, Pubmed , Echinobase
Nash, Elevated temperature attenuates ovarian functions and induces apoptosis and oxidative stress in the American oyster, Crassostrea virginica: potential mechanisms and signaling pathways. 2019, Pubmed
Olivares-Rubio, Lipid metabolism and pro-oxidant/antioxidant balance of Halamphora oceanica from the Gulf of Mexico exposed to water accommodated fraction of Maya crude oil. 2018, Pubmed
Pan, Influence of flow velocity on motor behavior of sea cucumber Apostichopus japonicus. 2015, Pubmed , Echinobase
Parisi, Antioxidative Defense and Fertility Rate in the Assessment of Reprotoxicity Risk Posed by Global Warming. 2019, Pubmed
Pasparakis, Effects of Deepwater Horizon crude oil exposure, temperature and developmental stage on oxygen consumption of embryonic and larval mahi-mahi (Coryphaena hippurus). 2016, Pubmed
Pasparakis, Combined effects of oil exposure, temperature and ultraviolet radiation on buoyancy and oxygen consumption of embryonic mahi-mahi, Coryphaena hippurus. 2017, Pubmed
Perrichon, Combined effects of elevated temperature and Deepwater Horizon oil exposure on the cardiac performance of larval mahi-mahi, Coryphaena hippurus. 2018, Pubmed
Perrichon, Morphology and cardiac physiology are differentially affected by temperature in developing larvae of the marine fish mahi-mahi (Coryphaena hippurus). 2017, Pubmed
Rahman, Effects of elevated temperature on prooxidant-antioxidant homeostasis and redox status in the American oyster: Signaling pathways of cellular apoptosis during heat stress. 2021, Pubmed
Rampon, Hydrogen Peroxide and Redox Regulation of Developments. 2018, Pubmed
Sanchez, A novel "Integrated Biomarker Response" calculation based on reference deviation concept. 2013, Pubmed
Sies, Oxidative Stress: Concept and Some Practical Aspects. 2020, Pubmed
Tairova, Effects of water accommodated fraction of physically and chemically dispersed heavy fuel oil on beach spawning capelin (Mallotus villosus). 2019, Pubmed
Telahigue, Mercury disrupts redox status, up-regulates metallothionein and induces genotoxicity in respiratory tree of sea cucumber (Holothuria forskali). 2020, Pubmed , Echinobase
Valavanidis, 8-hydroxy-2' -deoxyguanosine (8-OHdG): A critical biomarker of oxidative stress and carcinogenesis. 2009, Pubmed
Vieira, DNA damage and oxidative stress induced by imidacloprid exposure in different tissues of the Neotropical fish Prochilodus lineatus. 2018, Pubmed
Wang, Parental Effect of Long Acclimatization on Thermal Tolerance of Juvenile Sea Cucumber Apostichopus japonicus. 2015, Pubmed , Echinobase
Wang, Sex-Specific Differences in the Toxic Effects of Heavy Fuel Oil on Sea Urchin (Strongylocentrotus intermedius). 2021, Pubmed , Echinobase
Wang, The molecular mechanism of AhR-ARNT-XREs signaling pathway in the detoxification response induced by polycyclic aromatic hydrocarbons (PAHs) in clam Ruditapes philippinarum. 2020, Pubmed
Xiu, Bioaccumulation and oxidative damage in juvenile scallop Chlamys farreri exposed to benzo[a]pyrene, benzo[b]fluoranthene and chrysene. 2014, Pubmed
Yu, Cellular defenses against damage from reactive oxygen species. 1994, Pubmed
Zhang, Effects of acute ammonia toxicity on oxidative stress, DNA damage and apoptosis in digestive gland and gill of Asian clam (Corbicula fluminea). 2020, Pubmed
Zhou, Oxidative stress, apoptosis activation and symbiosis disruption in giant clam Tridacna crocea under high temperature. 2019, Pubmed