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ECB-ART-52159
Dev Growth Differ 1984 Jan 01;265:451-463. doi: 10.1111/j.1440-169X.1984.00451.x.
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Effect of Aminopterin and Deoxyribonucleosides on the Cleavage and Embryogenesis of the Sea Urchin, Hemicentrotus pulcherrimus: (aminopterin/thymidine/5-bromo-2'-deoxyuridine/cell division/sea urchin development).

Niikura K , Fujiwara A , Yasumasu I .


Abstract
In the embryos of the sea urchin, Hemicentrotus pulcherrimus, reared with 150 μM aminopterin from the time of fertilization, cessation of the development occurred at the blastula stage, at which the dTTP level became quite low. Another addition of thymidine to the embryo culture containing aminopterin resulted in an elevation of dTTP concentration in the embryos and allowed them to develop normally. Decrease in the dTTP level, resulting from the inhibition of thymidylate synthesis by aminopterin, probably causes a failure of egg cleavage and development. 5-Bromo-2'-deoxyuridine (BUdR) also released the aminopterin-inhibition of egg cleavage and allowed the treated embryos to develop to early gastrulae. Thereafter, the degeneration of archenteron occurred and these embryos became large permanent blastulae. Other deoxyribonucleosides failed to cancel the inhibition by aminopterin of egg cleavage. In the embryos kept with both BUdR and aminopterin, BUdR incorporation into DNA occurred at a similar rate as in thymidine incorporation in the embryos kept with thymidine and aminopterin, and was inhibited by another addition of thymidine. Without aminopterin treatment, BUdR incorporation hardly occurred and the embryos developed normally. BUdR incorporation into DNA in place of thymidine probably occurs in aminopterin-treated embryos, resulting in abnormal development.

PubMed ID: 37280794
Article link: Dev Growth Differ