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Dev Biol
2012 Apr 15;3642:259-67. doi: 10.1016/j.ydbio.2012.02.003.
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Cis-regulatory logic driving glial cells missing: self-sustaining circuitry in later embryogenesis.
Ransick A
,
Davidson EH
.
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The glial cells missing (gcm) regulatory gene of the sea urchin Strongylocentrotus purpuratus is first expressed in veg2 daughter cells as the genomic target of late cleavage stage Delta-Notch signaling from the skeletogenic mesoderm precursors. Gcm is required in veg2 progeny during late cleavages for the early phase of pigment cell precursor specification. Here we report on a later acting cis-regulatory module that assumes control of gcm expression by the early mesenchyme blastula stage and maintains it through pigment cell differentiation and dispersal. Cis-perturbation analyses reveal that the two critical elements within this late module are consensus matches to Gcm and Six1 binding sites. Significantly, six1 mRNA localizes to gcm+cells from the mesenchyme blastula stage onwards. Trans-perturbations with anti-sense morpholinos reveal a co-dependency between six1 and gcm. Six1 mRNA levels fall sharply after Gcm is depleted, while depleting Six1 leads to significant reductions in output of endogenous gcm or modular-reporters. These results support the conclusion gcm and six1 comprise a positive intergenic feedback loop in the mesodermal GRN. This often employed cross regulatory GRN feature here ensures self-sustaining gcm output in a cohort of fully specified pigment cell precursors at a relatively early developmental stage.
Arnone,
Using reporter genes to study cis-regulatory elements.
2004, Pubmed,
Echinobase
Arnone,
Using reporter genes to study cis-regulatory elements.
2004,
Pubmed
,
Echinobase
Brown,
Paircomp, FamilyRelationsII and Cartwheel: tools for interspecific sequence comparison.
2005,
Pubmed
Brown,
New computational approaches for analysis of cis-regulatory networks.
2002,
Pubmed
,
Echinobase
Calestani,
Cis-regulatory analysis of the sea urchin pigment cell gene polyketide synthase.
2010,
Pubmed
,
Echinobase
Calestani,
Isolation of pigment cell specific genes in the sea urchin embryo by differential macroarray screening.
2003,
Pubmed
,
Echinobase
Cameron,
A sea urchin genome project: sequence scan, virtual map, and additional resources.
2000,
Pubmed
,
Echinobase
Croce,
Dynamics of Delta/Notch signaling on endomesoderm segregation in the sea urchin embryo.
2010,
Pubmed
,
Echinobase
Davidson,
Spatial mechanisms of gene regulation in metazoan embryos.
1991,
Pubmed
Davidson,
Properties of developmental gene regulatory networks.
2008,
Pubmed
,
Echinobase
Davidson,
Regulatory gene networks and the properties of the developmental process.
2003,
Pubmed
Davidson,
Network design principles from the sea urchin embryo.
2009,
Pubmed
,
Echinobase
De Iaco,
Huckebein-mediated autoregulation of Glide/Gcm triggers glia specification.
2006,
Pubmed
Duboc,
Nodal and BMP2/4 pattern the mesoderm and endoderm during development of the sea urchin embryo.
2010,
Pubmed
,
Echinobase
Furukawa,
Defense system by mesenchyme cells in bipinnaria larvae of the starfish, Asterina pectinifera.
2009,
Pubmed
,
Echinobase
Gibson,
Migratory and invasive behavior of pigment cells in normal and animalized sea urchin embryos.
1987,
Pubmed
,
Echinobase
Gibson,
The origin of pigment cells in embryos of the sea urchin Strongylocentrotus purpuratus.
1985,
Pubmed
,
Echinobase
Hinman,
Developmental gene regulatory network architecture across 500 million years of echinoderm evolution.
2003,
Pubmed
,
Echinobase
Lee,
Expression of Spgatae, the Strongylocentrotus purpuratus ortholog of vertebrate GATA4/5/6 factors.
2004,
Pubmed
,
Echinobase
Lee,
Exclusive developmental functions of gatae cis-regulatory modules in the Strongylocentrorus purpuratus embryo.
2007,
Pubmed
,
Echinobase
Materna,
High accuracy, high-resolution prevalence measurement for the majority of locally expressed regulatory genes in early sea urchin development.
2010,
Pubmed
,
Echinobase
McCauley,
A conserved gene regulatory network subcircuit drives different developmental fates in the vegetal pole of highly divergent echinoderm embryos.
2010,
Pubmed
,
Echinobase
McMahon,
Introduction of cloned DNA into sea urchin egg cytoplasm: replication and persistence during embryogenesis.
1985,
Pubmed
,
Echinobase
Minokawa,
Expression patterns of four different regulatory genes that function during sea urchin development.
2004,
Pubmed
,
Echinobase
Newburger,
UniPROBE: an online database of protein binding microarray data on protein-DNA interactions.
2009,
Pubmed
Oliveri,
A regulatory gene network that directs micromere specification in the sea urchin embryo.
2002,
Pubmed
,
Echinobase
Oliveri,
Global regulatory logic for specification of an embryonic cell lineage.
2008,
Pubmed
,
Echinobase
Oliveri,
Gene regulatory network analysis in sea urchin embryos.
2004,
Pubmed
,
Echinobase
Poustka,
A global view of gene expression in lithium and zinc treated sea urchin embryos: new components of gene regulatory networks.
2007,
Pubmed
,
Echinobase
Ransick,
cis-regulatory processing of Notch signaling input to the sea urchin glial cells missing gene during mesoderm specification.
2006,
Pubmed
,
Echinobase
Ransick,
New early zygotic regulators expressed in endomesoderm of sea urchin embryos discovered by differential array hybridization.
2002,
Pubmed
,
Echinobase
Revilla-i-Domingo,
R11: a cis-regulatory node of the sea urchin embryo gene network that controls early expression of SpDelta in micromeres.
2004,
Pubmed
,
Echinobase
Rizzo,
Identification and developmental expression of the ets gene family in the sea urchin (Strongylocentrotus purpuratus).
2006,
Pubmed
,
Echinobase
Sandelin,
JASPAR: an open-access database for eukaryotic transcription factor binding profiles.
2004,
Pubmed
Shaner,
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.
2004,
Pubmed
Sherwood,
LvNotch signaling mediates secondary mesenchyme specification in the sea urchin embryo.
1999,
Pubmed
,
Echinobase
Sweet,
LvDelta is a mesoderm-inducing signal in the sea urchin embryo and can endow blastomeres with organizer-like properties.
2002,
Pubmed
,
Echinobase
Takata,
Behavior of pigment cells closely correlates the manner of gastrulation in sea urchin embryos.
2004,
Pubmed
,
Echinobase
Tuerk,
Protein stability and domain topology determine the transcriptional activity of the mammalian glial cells missing homolog, GCMb.
2000,
Pubmed
Warming,
Simple and highly efficient BAC recombineering using galK selection.
2005,
Pubmed
