Click
here to close Hello! We notice that
you are using Internet Explorer, which is not supported by Echinobase
and may cause the site to display incorrectly. We suggest using a
current version of Chrome,
FireFox,
or Safari.
Belenguer P
,
Caizergues-Ferrer M
,
Labbé JC
,
Dorée M
,
Amalric F
.
???displayArticle.abstract??? Nucleolin is a ubiquitous multifunctional protein involved in preribosome assembly and associated with both nucleolar chromatin in interphase and nucleolar organizer regions on metaphasic chromosomes in mitosis. Extensive nucleolin phosphorylation by a casein kinase (CKII) occurs on serine in growing cells. Here we report that while CKII phosphorylation is achieved in interphase, threonine phosphorylation occurs during mitosis. We provide evidence that this type of in vivo phosphorylation involves a mammalian homolog of the cell cycle control Cdc2 kinase. In vitro M-phase H1 kinase from starfish oocytes phosphorylated threonines in a TPXK motif present nine times in the amino-terminal part of the protein. The same sites which matched the p34cdc2 consensus phosphorylation sequence were used in vivo during mitosis. We propose that successive Cdc2 and CKII phosphorylation could modulate nucleolin function in controlling cell cycle-dependent nucleolar function and organization. Our results, along with previous studies, suggest that while serine phosphorylation is related to nucleolin function in the control of rDNA transcription, threonine phosphorylation is linked to mitotic reorganization of nucleolar chromatin.
Adams,
Hydroxyurea reversal of inhibition and use as a cell-synchronizing agent.
1967, Pubmed
Adams,
Hydroxyurea reversal of inhibition and use as a cell-synchronizing agent.
1967,
Pubmed
Allan,
The structure of histone H1 and its location in chromatin.
1980,
Pubmed
Arion,
cdc2 is a component of the M phase-specific histone H1 kinase: evidence for identity with MPF.
1988,
Pubmed
,
Echinobase
Belenguer,
Protein kinase NII and the regulation of rDNA transcription in mammalian cells.
1989,
Pubmed
Bourbon,
Structure of the mouse nucleolin gene. The complete sequence reveals that each RNA binding domain is encoded by two independent exons.
1988,
Pubmed
Bradbury,
Phosphorylation of very-lysine-rich histone in Physarum polycephalum. Correlation with chromosome condensation.
1973,
Pubmed
Bradford,
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
1976,
Pubmed
Brizuela,
Activation of human CDC2 protein as a histone H1 kinase is associated with complex formation with the p62 subunit.
1989,
Pubmed
Bugler,
Detection and localization of a class of proteins immunologically related to a 100-kDa nucleolar protein.
1982,
Pubmed
Bugler,
RNA binding fragments from nucleolin contain the ribonucleoprotein consensus sequence.
1987,
Pubmed
Caboche,
RNA methylation and control of eukaryotic RNA biosynthesis. Effects of cycloleucine, a specific inhibitor of methylation, on ribosomal RNA maturation.
1977,
Pubmed
Caizergues-Ferrer,
Phosphorylation of nucleolin by a nucleolar type NII protein kinase.
1987,
Pubmed
Cisek,
Phosphorylation of RNA polymerase by the murine homologue of the cell-cycle control protein cdc2.
1989,
Pubmed
Cooper,
Detection and quantification of phosphotyrosine in proteins.
1983,
Pubmed
De Brabander,
The effects of methyl (5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl) carbamate, (R 17934; NSC 238159), a new synthetic antitumoral drug interfering with microtubules, on mammalian cells cultured in vitro.
1976,
Pubmed
Dunphy,
The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis.
1988,
Pubmed
Earnshaw,
Anionic regions in nuclear proteins.
1987,
Pubmed
Elder,
Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein.
1977,
Pubmed
Erard,
A major nucleolar protein, nucleolin, induces chromatin decondensation by binding to histone H1.
1988,
Pubmed
Gas,
Immunolocalization of the 100 kDa nucleolar protein during the mitotic cycle in CHO cells.
1985,
Pubmed
Gautier,
Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2+.
1988,
Pubmed
Geahlen,
Induction of a substrate for casein kinase II during lymphocyte mitogenesis.
1984,
Pubmed
Giordano,
A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells.
1989,
Pubmed
Hayles,
A review of mitosis in the fission yeast Schizosaccharomyces pombe.
1989,
Pubmed
Heck,
In vivo phosphorylation of the 170-kDa form of eukaryotic DNA topoisomerase II. Cell cycle analysis.
1989,
Pubmed
Herrera,
Association of protein C23 with rapidly labeled nucleolar RNA.
1986,
Pubmed
Hohmann,
Phosphorylation of H1 histones.
1983,
Pubmed
Hopp,
Prediction of protein antigenic determinants from amino acid sequences.
1981,
Pubmed
Hunter,
Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
1980,
Pubmed
Huttner,
Differential phosphorylation of multiple sites in purified protein I by cyclic AMP-dependent and calcium-dependent protein kinases.
1981,
Pubmed
Kuenzel,
Substrate specificity determinants for casein kinase II as deduced from studies with synthetic peptides.
1987,
Pubmed
Labbe,
Activation at M-phase of a protein kinase encoded by a starfish homologue of the cell cycle control gene cdc2+.
1988,
Pubmed
,
Echinobase
Labbe,
Purification of MPF from starfish: identification as the H1 histone kinase p34cdc2 and a possible mechanism for its periodic activation.
1989,
Pubmed
,
Echinobase
Labbé,
MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B.
1989,
Pubmed
,
Echinobase
Langan,
Mammalian growth-associated H1 histone kinase: a homolog of cdc2+/CDC28 protein kinases controlling mitotic entry in yeast and frog cells.
1989,
Pubmed
Lapeyre,
Nucleolin, the major nucleolar protein of growing eukaryotic cells: an unusual protein structure revealed by the nucleotide sequence.
1987,
Pubmed
Matthews,
Nuclear protein kinases.
1984,
Pubmed
McVey,
Phosphorylation of large tumour antigen by cdc2 stimulates SV40 DNA replication.
1989,
Pubmed
Olson,
Distribution of proteins among chromatin components of nucleoli.
1983,
Pubmed
Pelech,
Characterization of maturation-activated histone H1 and ribosomal S6 kinases in sea star oocytes.
1987,
Pubmed
,
Echinobase
Peter,
Identification of major nucleolar proteins as candidate mitotic substrates of cdc2 kinase.
1990,
Pubmed
,
Echinobase
Pines,
Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2.
1989,
Pubmed
Schneider,
Enhanced casein kinase II activity during mouse embryogenesis. Identification of a 110-kDa phosphoprotein as the major phosphorylation product in mouse embryos and Krebs II mouse ascites tumor cells.
1986,
Pubmed
Shenoy,
Purified maturation promoting factor phosphorylates pp60c-src at the sites phosphorylated during fibroblast mitosis.
1989,
Pubmed
Srivastava,
Cloning and sequencing of the human nucleolin cDNA.
1989,
Pubmed
Suzuki,
Effects of androgen and polyamines on the phosphorylation of nucleolar proteins from rat ventral prostates with particular reference to 110-kDa phosphoprotein.
1985,
Pubmed
Uemura,
Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization.
1984,
Pubmed
Uemura,
The nucleotide sequence of the fission yeast DNA topoisomerase II gene: structural and functional relationships to other DNA topoisomerases.
1986,
Pubmed
Westendorf,
The role of cyclin B in meiosis I.
1989,
Pubmed
Woodford,
Histone H1 kinase in exponential and synchronous populations of Chinese hamster fibroblasts.
1986,
Pubmed
Zalta,
Isolation of nucleoli. A method that combines high yield, structural integrity, and biochemical preservation.
1971,
Pubmed
Zandomeni,
A rapid purification method for calf thymus casein kinase II.
1988,
Pubmed
Zingde,
Peptide mapping of proteins in gel bands after partial cleavage with acidic cyanogen bromide vapors.
1986,
Pubmed
